Wyniki wyszukiwania - Biblioteka Nauki

1

Simultaneous quantification of five lignans from Schisandra chinensis in various tissues of rats

100%

Su L.-L. , Cheng X. , Ding X.-Y. , Mao C.-Q. , Lu T.-L. , Hao M. , Li B.-W. , Qin S.

Acta Chromatographica

|

2019

|

tom Vol. 31, no. 2

113--119

EN

In this research study, a rapid, sensitive, and specific high-performance liquid chromatography–electrospray ionization–tandem mass spectrometry (HPLC–ESI–MS/MS) method was established and validated, in regard to the simultaneous quantification of five sedative and hypnotic lignans (schisandrin, schisandrol B, schisantherin A, deoxyschisandrin, and schisandrin B) in various tissues of rats (including heart, liver, spleen, lung, and kidney). The purpose of the study was to clarify the tissue distribution of the total lignans extract of Schisandra chinensis (SC). Then, the analytes were separated on a MERCK Purospher STAR LP C18 column (250 mm × 4.6 mm, 5 μm), with a mobile phase consisting of 0.05% (v/v) formic acid acetonitrile, and 0.05% (v/v) formic acid water, and a flow rate of 1 mL/min. All of the calibration curves of the five components showed good linearity (r > 0.9950), with ranges of 4.8 to 1920 ng/mL for analytes. The intra-day and inter-day precisions (relative standard deviation [RSD] %) were within 13.76% for all of the analytes. The average recoveries of the five analytes were greater than 85.23%, and the mean value of the matrix effect ranged from 82.3% to 93.4%. The five analytes were confirmed to be stable during the storage, preparation, and analytic procedures. The major target tissues of the total lignans extract of the SC in the rats were the livers and kidneys.

2

Expression level of Ubc9 protein in rat tissues.

100%

Gołębiowski F. , Szulc A. , Sakowicz M. , Szutowicz A. , Pawełczyk T.

Acta Biochimica Polonica

|

2003

|

tom 50

|

nr 4

1064-1073

EN

Ubc9 is a homologue of the E2 ubiquitin conjugating enzyme and participates in the covalent linking of SUMO-1 molecule to the target protein. In this report we describe a simple and efficient method for obtaining pure human recombinant Ubc9 protein. The purified Ubc9 retained its native structure and was fully active in an in vitro sumoylation assay with the promyelocytic leukaemia (PML) peptide as a substrate. In order to better understand the physiology of Ubc9 protein we examined its levels in several rat tissues. Immunoblot analyses performed on tissue extracts revealed quantitative and qualitative differences in the expression pattern of Ubc9. The Ubc9 protein was present at a high level in spleen and lung. Moderate level of Ubc9 was detected in kidney and liver. Low amount of Ubc9 was observed in brain, whereas the 18 kDa band of Ubc9 was barely visible or absent in heart and skeletal muscle. In heart and muscle extracts the Ubc9 antibodies recognized a 38 kDa protein band. This band was not visible in extracts of other rat tissues. A comparison of the relative levels of Ubc9 mRNA and protein indicated that the overall expression level of Ubc9 was the highest in spleen and lung. In spleen, lung, kidney, brain, liver and heart there was a good correlation between the 18 kDa protein and Ubc9 mRNA levels. In skeletal muscle the Ubc9 mRNA level was unproportionally high comparing to the level of the 18 kDa protein. The presented data indicate that in the rat the expression of the Ubc9 protein appears to have some degree of tissue specificity.

3

Tissue distribution of a menthyl-conjugated oligodeoxyribonucleotide antisense to PAI-1 mRNA

100%

Szemraj J. , Khalid N.I Al-Nedawi , Chabielska E. , Buczko W. , Pawlowska Z.

Acta Biochimica Polonica

|

2005

|

tom 52

|

nr 4

849-855

EN

The inhibitory effect of numerous analogues of PO-16, an hexadecadeoxyribonucleotide antisense to sequences -22 to -17 of PAI-1 mRNA coding for a fragment of the signal peptide, on the expression of PAI-1 in endothelial cells, and physiological consequences of the subsequently reduced PAI-1 activity tested in vitro and in vivo, were described in our previous studies. Of particular interest was PO-16 5'-O-conjugated with menthyl phosphorothioate (MPO-16R). In this work, tissue localisation of MPO-16R labelled with [35S] phosphorothioate at the 3'-end, was determined. [35S]MPO-16R and control [35S]MPO-16R-SENSE oligonucleotides were administered intravenously into 22 rats and organ distribution of the labelled bioconjugates was assessed after 24 and 48 h. For this purpose, tissue sections were subjected to autoradiography, and quantitated by liquid scintillation after solubilisation. Overall clearance of radioactivity was already seen after 24 h, with the radioactivity recovered mainly in the kidney and liver. A smaller fraction of radioactivity was also retained in the spleen and heart. The kidney concentration of the labelled probe was higher than that of liver by 50%. The distribution of PAI-1 mRNA in untreated rat kidney, liver, spleen and heart established by two independent techniques: Ribonuclease Protection Assay and Real-Time PCR, shows the same pattern as that observed for [35S]MPO-16R antisense.

4

Body composition and fatty tissue distribution in women with various menstrual status

88%

Dmitruk A. , Czeczelewski J. , Czeczelewska E. , Golach J. , Parnicka U.

Roczniki Państwowego Zakładu Higieny

|

2018

|

tom 69

|

nr 1

EN

Background. Menopause, also referred to as climacterium, is a period of multiple changes in the structure and functions of a woman organism. Objective. Determination of differences in body composition and fatty tissue distribution in women from groups discriminated based on their menstrual status. Material and Methods. The survey covered 312 women aged 38-75 years. Menstrual status of the surveyed women was established according to WHO guidelines based on answers to a questionnaire, and three groups were discriminated: women in the premenopausal period (group 1), in the perimenopausal period (group 2), and in the postmenopausal period (group 3). The following anthropomological measurements were taken: body height, body mass, waist and hip circumference, and thickness of 6 skinfolds. Their results enabled evaluating the somatic built of women in the separated groups. Fatty tissue distribution was determined based on TER distribution index calculated as a ratio of the sum of trunk skinfolds (TSS) to the sum of extremity skinfolds (ESS). Body composition of the women, including percentage of body fat, lean body mass, soft tissue mass, and total body water, was assessed using an IOI 353 analyzer by JAWON MEDICAL. In addition, percentages of women with underweight, normal content of fatty tissue, and these with overweight and obesity were calculated. The WHR index was computed in the case of obese women. Results. The highest values of body mass, hip circumference and most of the skinfolds were determined in the perimenopausal group, whereas the postmenopausal women were characterized by the highest percentage of body fat (PBF) and by the lowest contents of lean tissue, soft tissue, and total water content in the body. The highest percentage of obese women was found in the postmenopausal group, including 40% of them having visceral type obesity. The occurrence of the menopause contributed to changes in fatty tissue distribution, causing its shift from extremities toward the trunk. Conclusions. The study showed differences in the somatic built and body composition in groups of women distinguished based on their menstrual status.

PL

Wprowadzenie. Menopauza, zwana inaczej przekwitaniem, to okres licznych zmian w budowie i funkcjonowaniu organizmu kobiety. Cel. Określenie wielkości różnic w składzie ciała i rozmieszczeniu tkanki tłuszczowej u kobiet w grupach wydzielonych na podstawie statusu menstruacyjnego. Materiał i metody. Badaniom poddano 312 kobiet w wieku 38-75 lat. Na podstawie odpowiedzi udzielonych na pytania ankiety określono status menstruacyjny badanych kobiet zgodnie z zaleceniami WHO. Wydzielono trzy grupy kobiet będących w okresie premenopauzalnym (grupa 1- 69 kobiet ), perimenopauzalnym (grupa 2 – 45 kobiet) i postmenopauzalnym (grupa 3- 198 kobiet). Wykonano pomiary antropologiczne: wysokości ciała, masy ciała, obwodu pasa i bioder oraz grubości 6 fałdów skórno-tłuszczowych w celu oceny budowy somatycznej kobiet w wydzielonych grupach. W celu określenia dystrybucji tkanki tłuszczowej obliczono wskaźnik dystrybucji TER, będący stosunkiem sumy fałdów skórno-tłuszczowych na tułowiu (TSS) do sumy fałdów skórno-tłuszczowych na kończynach (ESS). Skład ciała oceniono przy pomocy analizatora składu ciała IOI 353 z oprogramowaniem JAWON MEDICAL. Pozwoliło to na określenie m.in. procentowej zawartości tkanki tłuszczowej, beztłuszczowej masy ciała, masy tkanek miękkich oraz całkowitej zawartości wody. Obliczono również odsetek osób z niedowagą, prawidłową zawartością tkanki tłuszczowej oraz z nadwagą i otyłością. U otyłych kobiet obliczono wskaźnik WHR. Wyniki. Najwyższe wartości masy ciała, obwodu bioder i większości fałdów skórno-tłuszczowych wystąpiły w grupie perimenopauzalnej, a w grupie postmenopauzalnej – najwyższe wartości całkowitej zawartości tkanki tłuszczowej (PBF), przy równoczesnym najniższym poziomie tkanki beztłuszczowej, tkanek miękkich i całkowitej zawartości wody w organizmie. Najwyższy odsetek otyłych kobiet wystąpił w grupie postmenopauzalnej, przy czym u 40% badanych z tej grupy była to otyłość wisceralna. Wystąpienie menopauzy przyczyniło się do zmian w rozmieszczeniu tkanki tłuszczowej, powodując przesunięcie jej z kończyn w kierunku tułowia. Wnioski. Stwierdzono występowanie różnic w budowie somatycznej i składzie ciała kobiet w grupach wydzielonych na podstawie statusu menstruacyjnego.

5

Pharmacokinetics of panasenoside in rats and tissue distribution in mice by ultra-performance liquid chromatography tandem mass spectrometry

88%

Chen R. , Lu M. , Tu X. , Sun W. , Ye W. , Ma J. , Wen C. , Wang X. , Geng P.

Acta Chromatographica

|

2019

|

tom Vol. 31, no. 2

146--150

EN

We developed an ultra-performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) method for quantification of panasenoside pharmacokinetics in rat plasma and tissue distribution in mouse. Twelve male Sprague-Dawley rats were used for pharmacokinetics after intravenous (2 or 10 mg/kg) administration of panasenoside, six rats for each dose. Thirty mice were randomly divided into six groups (five mice for each group, one group for each time point) and received 20 mg/kg of panasenoside by intraperitoneal administration. Calibration plots were in the range of 2–2000 ng/mL for panasenoside in rat plasma and 2–3000 ng/mL in mouse tissues. The relative standard deviation (RSD) of inter-day and intra-day precision was less than 14%. The accuracy was between 89.6% and 110.0%. The AUC(0-t) was 160.8 ± 13.0 and 404.9 ± 78.0 ng/mL*h, and t1/2 of 3.2 ± 1.2 and 4.6 ± 1.7 h, CL (clearance) of 10.0 ± 2.0, and 21.4 ± 2.0 L/h/kg after intravenous administration 2 mg/kg and 10 mg/kg of panasenoside, respectively. The tissue distribution results indicated that the panasenoside diffuses rapidly and widely into major organs. The level of panasenoside was highest in mouse liver, followed by kidney, lung, and spleen. The overwhelming accumulation in liver indicated that liver was responsible for the extensive metabolism.

6

Study on pharmacokinetics, tissue distribution, and excretion of phloretin and its prodrug 2′,4′,6′,4-Tetra-O-acetylphloretin in rats using LC–MS/MS

88%

Wang L. , Li X. , Mi L. , Shen X. , Feng T. , Liu X. , Wang Q.

Acta Chromatographica

|

2019

|

tom Vol. 31, no. 1

63--70

EN

2′,4′,6′,4-Tetra-O-acetylphloretin (TAPHL) is a prodrug of phloretin (PHL) in which the OH groups are protected by acetylation. A validated liquid chromatography–tandem mass spectrometry (LC–MS/MS) method for the determination of PHL in rat biological matrices was developed and applied to investigate and compare the pharmacokinetics, tissue distribution, and excretion of PHL and TAPHL in rats following a single oral administration. The method was validated for accuracy, precision, linearity, range, selectivity, lower limit of quantification (LLOQ), recovery, and matrix effect. All validation parameters met the acceptance criteria according to regulatory guidelines. The mean pharmacokinetic parameters of tmax, Cmax, AUC(0 − t), CL/F, and t1/2 were observed after oral administration in rats. The data showed that PHL was absorbed and eliminated rapidly from plasma after oral administration. The pharmacokinetic properties are improved, such as the tmax has been prolonged and the area under the curve (AUC) has been enhanced after oral administration of TAPHL to rats. Tissue distribution results indicated that PHL could be rapidly and widely distributed into tissues but could not effectively cross the blood–brain barrier in rats. After oral administration of TAPHL to rats, its tissue distribution to rats was similar as that after oral administration of equimolar PHL. In addition, higher recoveries of PHL following administration of TAPHL indicated that TAPHL might reduce the excretion of PHL from the body by reducing the first pass effect.

7

Polybrominated diphenyl ethers (PBDEs) in sediment and fish tissues from Lake Chaohu, central eastern China

88%

Yang S. , Fu Q. , Teng M. , Yang J.

Archives of Environmental Protection

|

2015

|

tom Vol. 41, no. 2

12--20

EN

Polybrominated diphenyl ethers (PBDEs) levels in environmental media have increased over the last 20-25 years in the world. In aquatic environments PBDEs were found to be accumulated along food chain and Endocrine disruptors toxicity. In this study PBDEs were investigated in sediment and fish tissues from Lake Chaohu in central eastern China. There were 10 PBDEs congeners detected out of all 41 PBDEs. BDE-47 was of the highest with 5.17 ng/g in sediment and 58.47 ng/g in fish. PBDEs were evenly distributed across the surface sediment in the whole lake. It implied that the main source of PBDEs may not be an inflow river like Nanfei. Tissue distribution patterns of PBDEs in four fish species were in the order of BDE-47 > BDE-99 > BDE-100 > BDE-66 > BDE-138 > BDE-183 > BDE-154 > BDE-153. Octa- and deca-BDEs were below the detection limit. The concentrations of all PBDE congeners were higher in gills, livers, and kidneys than those in muscles and adipose tissue. Furthermore, PBDEs in different tissues had some different distribution patterns with fish size. Those discrepancies appeared to be correlated with the PBDEs pollution fluxes varying with the change of the year and their metabolism divergences in fish tissues.

8

Isolation and characterization of pigeon breast muscle cytosolic 5´-nucleotidase-I (cN-I)

88%

Tkacz-Stachowska K. , Lechward K. , Skladanowski A. C.

Acta Biochimica Polonica

|

2005

|

tom 52

|

nr 4

789-796

EN

5´-Nucleotidase specific towards dCMP and AMP was isolated from avian breast muscle and characterized. It was found to be similar to a type-I form (cN-I) identified earlier as the AMP-selective 5´-nucleotidase responsible for adenosine formation during ATP breakdown in transfected COS-7 cells. Expression pattern of the cN-I gene in pigeon tissues indicated breast muscle as a rich source of the transcript. We purified the enzyme from this source using two-step chromatography and obtained an active homogenous preparation, free of ecto-5´-nucleotidase activity. The tissue content of the activity was calculated at 0.09 U/g wet weight. The specific activity of the enzyme preparation was 4.33 U/mg protein and it preferred dCMP and AMP to dAMP and IMP as a substrate. Its kinetic properties were very similar to those of the enzyme purified earlier from heart tissue. It was strongly activated by ADP. Inhibition by inorganic phosphate was more pronounced than in heart-isolated cN-I. Despite this difference, a similar physiological function is suggested for cN-I in both types of muscle.

9

Pharmacokinetics of ligustroflavone in rats and tissue distribution in mice by UPLC–MS/MS

88%

Zhou Q. , Zhang Z. , Geng P. , Huang B. , Wang X. , Yu X.

Acta Chromatographica

|

2020

|

tom Vol. 32, no. 2

102--106

EN

An ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) method was developed and validated for quantification of ligustroflavone, which was then applied in pharmacokinetics study in rat and tissue distribution in mouse. Twelve male Sprague Dawley rats were used for pharmacokinetics after intravenous (2 or 8 mg/kg) administration of ligustroflavone, six rats for each dose. Twenty-five mice were randomly divided into 5 groups (5 mice for each group, 1 group for each time point) and received 16 mg/kg ligustroflavone via intraperitoneal administration. The linear range of the calibration curve was over 2–2000 ng/mL for ligustroflavone in rat plasma and mouse tissues. The intra-day and inter-day precision expressed in % RSD were less than 14%, and the accuracy was between 88.5% and 108.4%.

10

Expression level of adenosine kinase in rat tissues. Lack of phosphate effect on the enzyme activity.

88%

Sakowicz M. , Grdeń M. , Pawełczyk T.

Acta Biochimica Polonica

|

2001

|

tom 48

|

nr 3

745-754

EN

In this report we describe cloning and expression of rat adenosine kinase (AK) in Esccherichaia coli cells as a fusion protein with 6xHis. The recombinant protein was purified and polyclonal antibodies to AK were generated in rabbits. Immunoblot analysis of extracts obtained from various rat tissues revealed two protein bands reactive with anti-AK IgG. The apparent molecular mass of these bands was 48 and 38 kDa in rat kidney, liver, spleen, brain, and lung. In heart and muscle the proteins that react with AK antibodies have the molecular masses of 48 and 40.5 kDa. In order to assess the relative AK mRNA level in rat tissues we used the multiplex PCR technique with β-actin mRNA as a reference. We found the highest level of AK mRNA in the liver, which decreased in the order kidney >spleen >lung >heart >brain >muscle. Measurement of AK activity in cytosolic fractions of rat tissues showed the highest activity in the liver (0.58 U/g), which decreased in the order kidney >spleen >lung >brain >heart >skeletal muscle. Kinetic studies on recombinant AK as well as on AK in the cytosolic fraction of various rat tissues showed that this enzyme is not affected by phosphate ions. The data presented indicate that in the rat tissues investigated at least two isoforms of adenosine kinase are expressed, and that the expression of the AK gene appears to have some degree of tissue specificity.

11

Iron-superoxide dismutase from pepper: biochemical characterization, cell localization and tissue distribution

75%

Rodriguez-Ruiz M. , Alvarez de Morales P. , del Rio L. , Corpas F. , Palma J.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2013

|

tom 94

|

nr 2

12

Recent advances in studies on biochemical and structural properties of equilibrative and concentrative nucleoside transporters

75%

Podgorska M. , Kocbuch K. , Pawelczyk T.

Acta Biochimica Polonica

|

2005

|

tom 52

|

nr 4

749-758

EN

Nucleoside transporters (NT) facilitate the movement of nucleosides and nucleobases across cell membranes. NT-mediated transport is vital for the synthesis of nucleic acids in cells that lack de novo purine synthesis. Some nucleosides display biological activity and act as signalling molecules. For example, adenosine exerts a potent action on many physiological processes including vasodilatation, hormone and neurotransmitter release, platelet aggregation, and lipolysis. Therefore, carrier-mediated transport of this nucleoside plays an important role in modulating cell function, because the efficiency of the transport processes determines adenosine availability to its receptors or to metabolizing enzymes. Nucleoside transporters are also key elements in anticancer and antiviral therapy with the use of nucleoside analogues. Mammalian cells possess two major nucleoside transporter families: equilibrative (ENT) and concentrative (CNT) Na+-dependent ones. This review characterizes gene loci, substrate specificity, tissue distribution, membrane topology and structure of ENT and CNT proteins. Regulation of nucleoside transporters by various factors is also presented.

13

Isolation and characterization of pigeon breast muscle cytosolic 5'-nucleotidase-I [cN-I]

75%

Tkacz-Stachowska K. , Lechward K. , Skladanowski A. C.

Acta Biochimica Polonica

|

2005

|

tom 52

|

nr 4

EN

5´-Nucleotidase specific towards dCMP and AMP was isolated from avian breast muscle and characterized. It was found to be similar to a type-I form (cN-I) identified earlier as the AMP-selective 5´-nucleotidase responsible for adenosine formation during ATP breakdown in transfected COS-7 cells. Expression pattern of the cN-I gene in pigeon tissues indicated breast muscle as a rich source of the transcript. We purified the enzyme from this source using two-step chromatography and obtained an active homogenous preparation, free of ecto-5´-nucleotidase activity. The tissue content of the activity was calculated at 0.09 U/g wet weight. The specific activity of the enzyme preparation was 4.33 U/mg protein and it preferred dCMP and AMP to dAMP and IMP as a substrate. Its kinetic properties were very similar to those of the enzyme purified earlier from heart tissue. It was strongly activated by ADP. Inhibition by inorganic phosphate was more pronounced than in heart-isolated cN-I. Despite this difference, a similar physiological function is suggested for cN-I in both types of muscle.

14

Tissue distribution of a menthyl-conjugated oligodeoxyribonucleotide antisense to PAI-1 mRNA

75%

Szemraj J. , Al-Nedawi K. N. I. , Chabielska E. , Buczko W. , Pawlowska Z.

Acta Biochimica Polonica

|

2005

|

tom 52

|

nr 4

EN

The inhibitory effect of numerous analogues of PO-16, an hexadecadeoxyribonucleotide antisense to sequences -22 to -17 of PAI-1 mRNA coding for a fragment of the signal peptide, on the expression of PAI-1 in endothelial cells, and physiological consequences of the subsequently reduced PAI-1 activity tested in vitro and in vivo, were described in our previous studies. Of particular interest was PO-16 5'-O-conjugated with menthyl phosphorothioate (MPO-16R). In this work, tissue localisation of MPO-16R labelled with [35S] phosphorothioate at the 3'-end, was determined. [35S]MPO-16R and control [35S]MPO-16R-SENSE oligonucleotides were administered intravenously into 22 rats and organ distribution of the labelled bioconjugates was assessed after 24 and 48 h. For this purpose, tissue sections were subjected to autoradiography, and quantitated by liquid scintillation after solubilisation. Overall clearance of radioactivity was already seen after 24 h, with the radioactivity recovered mainly in the kidney and liver. A smaller fraction of radioactivity was also retained in the spleen and heart. The kidney concentration of the labelled probe was higher than that of liver by 50%. The distribution of PAI-1 mRNA in untreated rat kidney, liver, spleen and heart established by two independent techniques: Ribonuclease Protection Assay and Real-Time PCR, shows the same pattern as that observed for [35S]MPO-16R antisense.

15

Identification and tissue distribution of a novel rat glucocorticoid receptor splice variant

63%

Ju H. , Wang X. , Liu Z. , Liu P. , Zhao Y. , Xiong R. , Zhou Y. , Chen X.

Acta Biochimica Polonica

|

2009

|

tom 56

|

nr 1

109-113

EN

Glucocorticoid receptor (GR) is a steroid hormone receptor that has been shown to play important roles in diverse cellular and physiological processes. More and more evidence has revealed that the effects of glucocorticoids are mediated by the glucocorticoid receptor through genomic or nongenomic mechanisms. A growing number of glucocorticoid receptor splice variants have been identified in human tissues, but few are known in rat tissues. In this work, a novel rGR cDNA, called rGRβ, was cloned from Sprague Dawlay (SD) rat liver. Sequence analysis revealed that the rGRβ mRNA was 39 base pairs (bp) shorter than the rGR mRNA reported earlier. The deleted segment is located in exon 1 and encodes 13 repeated glutamine residues. Both the rGR and rGRβ mRNAs were quantitated by Northern blot hybridization using non-homologous glucocorticoid cDNA probes. Results showed that the rGR and rGRβ mRNAs were most abundant in the lung, the least abundant in the heart, and there were more rGR and rGRβ mRNAs in the kidney than in the liver. The identification of rGRβ may contribute to the understanding of the genomic or nongenomic effects of glucocorticoids.

16

Quantitative determination and localization of cathepsin D and its inhibitors

63%

Minarowska A. , Karwowska A. , Gacko M.

Folia Histochemica et Cytobiologica

|

2009

|

tom 47

|

nr 2

153-177

17

Tissue distribution of the activity of inorganic pyrophosphatase in Helix pomatia L., H. aspersa O. F. Mull. (Gastropoda: Pulmonata: Helicidae) and Ampullaria cuprina (O.F. Mull.) (Gastropoda: Prosobranchia: Ampullariidae)

63%

Kozubek Z.

Folia Malacologica

|

1998

|

tom 06

|

nr 1-4

18

In vivo behavior and disposition of 3H-cholesterol ether labeled liposomes following intravenous administration to mice: comparison with an incorporated 14C-inulin as an aqueous phase marker

63%

Anderson M. , Paradis C. , Omri A.

Cellular and Molecular Biology Letters

|

2002

|

tom 07

|

nr 2

19

Tissue distribution of MHC class II-positive cells, their down-manipulation by monoclonal antibodies and potential role in organ allograft immunogenicity

63%

Krzymanski M. , Muller-Ruchholtz W.

Archivum Immunologiae et Therapiae Experimentalis

|

1992

|

tom 40

|

nr 3-4

20

Characterization of a novel transcript variant of human STAU1 gene

51%

Nie F. , Yao H. , Qi R. , Li X. , Wu C.

Acta Biochimica Polonica

|

2008

|

tom 55

|

nr 3

473-478

EN

Human STAU1 is one member of the family of double-stranded RNA (dsRNA)-binding proteins. It is thought to function in transporting mRNA, controlling translation and eliciting mRNA decay in neurons, and to function in infection of influenza virus and human immunodeficiency virus type 1 (HIV-1). Four transcripts coding two isoforms have been identified before. In this study, we have isolated a novel transcript of STAU1, coding a novel isoform that has six amino acids more (SFPLKQ) than isoform a. In order to examine the tissue distribution of this novel isoform, we have performed RT-PCR experiments and the analysis showed that it was highly expressed in heart, liver, kidney and pancreas.