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1
Content available Studies of polypropylene membrane fouling during microfiltration of broth with Citrobacter freundii bacteria
100%
Gryta M. , Waszak M. , Tomaszewska M.
Polish Journal of Chemical Technology
|
2015
|
tom Vol. 17, nr 4
56--64
EN
In this work a fouling study of polypropylene membranes used for microfiltration of glycerol solutions fermented by Citrobacter freundii bacteria was presented. The permeate free of C. freundii bacteria and having a turbidity in the range of 0.72–1.46 NTU was obtained. However, the initial permeate flux (100–110 L/m2h at 30 kPa of transmembrane pressure) was decreased 3–5 fold during 2–3 h of process duration. The performed scanning electron microscope observations confirmed that the filtered bacteria and suspensions present in the broth formed a cake layer on the membrane surface. A method of periodical module rinsing was used for restriction of the fouling influence on a flux decline. Rinsing with water removed most of the bacteria from the membrane surface, but did not permit to restore the initial permeate flux. It was confirmed that the irreversible fouling was dominated during broth filtration. The formed deposit was removed using a 1 wt% solution of sodium hydroxide as a rinsing solution.
2
Content available remote The study of glycerol-based fermentation and broth downstream by nanofiltration
100%
Gryta M. , Markowska-Szczupak A. , Grzechulska-Damszel J. , Bastrzyk J. , Waszak M.
Polish Journal of Chemical Technology
|
2014
|
tom 16
|
nr 4
117-122
EN
In this work, the glycerol fermentation was carried out using Citrobacter freundii bacteria. The influence of glycerol and metabolites concentrations, and the pH changes on the efficiency of 1,3-propanediol production, during batch and fed-batch processes, was presented. The nanofiltration was used for the separation of obtained post-fermentation solutions. The resulted 1,3-PD solutions were significantly desalted, which may facilitate further downstream processes during 1,3-PD production.
3
Content available remote Hypothetical glycerol pathways of newly isolated strains capable of 1,3-propanediol production
100%
Leja K. , Samul D. , Drożdżyńska A. , Myszka K. , Juzwa W. , Pawlicka J. , Czaczyk K.
Acta Biochimica Polonica
|
2014
|
tom 61
|
nr 4
759-763
EN
Study presented here demonstrates the ability of three newly isolated strains, obtained from environmental probes (manure, bottom sediment, and food waste) and identified as Clostridium bifermentans, Clostridium butyricum, and Hafnia alvei, to synthesize 1,3-propanediol (1,3-PD), organic acids (such as lactic, acetic, fumaric, succinic, and butyric acids), and ethanol from glycerol. The production of 1,3-PD as well as the glycerol pathways in C. bifermentans and H. alvei cells have not been investigated and described yet by others. Moreover, there is no data in the available literature on the products of glycerol utilization by H. alvei and there is only some incoherent data (mainly from the first half of the twentieth century) about the ability of C. bifermentans to carry out glycerol degradation. Additionally, this study presents complete hypothetical glycerol pathways and the basic fermentation kinetic parameters (such as yield and productivity) for both strains as well as for the newly isolated C. butyricum strain.
4
Content available The study of glycerol-based fermentation and broth downstream by nanofiltration
100%
Gryta M. , Markowska-Szczupak A. , Grzechulska-Damszel J. , Bastrzyk J. , Waszak M.
Polish Journal of Chemical Technology
|
2014
|
tom Vol. 16, nr 4
117--122
EN
In this work, the glycerol fermentation was carried out using Citrobacter freundii bacteria. The influence of glycerol and metabolites concentrations, and the pH changes on the efficiency of 1,3-propanediol production, during batch and fed-batch processes, was presented. The nanofiltration was used for the separation of obtained post-fermentation solutions. The resulted 1,3-PD solutions were significantly desalted, which may facilitate further downstream processes during 1,3-PD production.
5
Content available remote Separation of 1,3-Propanediol from Aqueous Solutions by Ion Exchange Chromatography
88%
Rukowicz B. , Miesiąc I. , Alejski K.
Polish Journal of Chemical Technology
|
2014
|
tom 16
|
nr 2
82-86
EN
1,3-propanediol is a promising monomer with many applications and can be produced by bioconversion of renewable resources. The separation of this product from fermentation broth is a difficult task. In this work, the application of cation exchange resin for the separation of 1,3-propanediol from model aqueous solution was examined. The best effect of separation of 1,3-propanediol from glycerol using sorption method was obtained for H+ resin form, although the observed partition coefficient of 1,3-propanediol was low. On the basis of the results of the sorption of 1,3-propanediol, the ionic forms of the resin were selected and used in the next experiments (H+, Ca2+, Ag+, Na+, Pb2+, Zn2+). The best results in ion exchange chromatography were obtained for cation exchange resin in H+ and Ca2+ form. The use of smaller particle size of resin and a longer length of the column allows to obtain better separation of mixtures.
6
Content available Separation of 1,3-Propanediol from Aqueous Solutions by Ion Exchange Chromatography
88%
Rukowicz B. , Miesiąc I. , Alejski K.
Polish Journal of Chemical Technology
|
2014
|
tom Vol. 16, nr 2
82--86
EN
1,3-propanediol is a promising monomer with many applications and can be produced by bioconversion of renewable resources. The separation of this product from fermentation broth is a difficult task. In this work, the application of cation exchange resin for the separation of 1,3-propanediol from model aqueous solution was examined. The best effect of separation of 1,3-propanediol from glycerol using sorption method was obtained for H+ resin form, although the observed partition coefficient of 1,3-propanediol was low. On the basis of the results of the sorption of 1,3-propanediol, the ionic forms of the resin were selected and used in the next experiments (H+, Ca2+, Ag+, Na+, Pb2+, Zn2+). The best results in ion exchange chromatography were obtained for cation exchange resin in H+ and Ca2+ form. The use of smaller particle size of resin and a longer length of the column allows to obtain better separation of mixtures.
7
Content available remote 1,3-propanediol production by Escherichia coli expressing genes of dha operon from Clostridium butyricum 2CR371.5
75%
Dąbrowski S. , Pietrewicz-Kubicz D. , Zabłotna E. , Długołęcka A.
Acta Biochimica Polonica
|
2012
|
tom 59
|
nr 3
357-361
EN
1,3-propanediol is used as a monomer in the production of some polymers e.g. polytrimethylene terephthalate used in the production of carpets and textile fibers and in the thermoplastics engineering. However, the traditional chemical synthesis is expensive, generates some toxic intermediates and requires a reduction step under high hydrogen pressure. Biological production of 1,3-propanediol could be an attractive alternative to the traditional chemical methods. Moreover, crude glycerol which is a by-product of biodiesel production, can be used. We constructed a recombinant Escherichia coli strain producing 1,3-propanediol from glycerol by introducing genes of the dha operon from Clostridium butyricum 2CR371.5, a strain from our collection of environmental samples and strains. The E. coli strain produced 3.7 g of 1,3-propanediol per one litre of culture with the yield of 0.3 g per 1 g of glycerol consumed.
8
Content available remote Screening of environmental samples for bacteria producing 1,3-propanediol from glycerol
75%
Dąbrowski S. , Zabłotna E. , Pietrewicz-Kubicz D. , Długołęcka A.
Acta Biochimica Polonica
|
2012
|
tom 59
|
nr 3
353-356
EN
Twenty nine environmental samples were screened for the presence of anaerobic microorganisms fermenting glycerol with 1,3-propanediol as a final product. Seven samples were then selected for the next step of our research and eight bacteria strains were cultured anaerobically. Seven of them produced 1,3-propanediol with a yield of 0.47-0.58. Six of the the isolated microorganisms were then classified as Clostridium butyricum (four strains), C. lituseburense (one strain), and C. sartagoforme (one strain). We suggest that of all these strains C. butyricum 2CR371.5 is the best 1,3-propanediol producer as producing no lactate as a by-product and growing well on a glycerol-containing medium.
9
Content available remote Wydzielanie 1,3-propanodiolu z brzeczki fermentacyjnej metodą ekstrakcji reaktywnej
75%
Alejski K. , Miesiąc I. , Rukowicz B. , Szóstak M.
Przemysł Chemiczny
|
2017
|
tom T. 96, nr 5
1152--1155
PL
Przeprowadzono badania ekstrakcji reaktywnej 1,3-propanodiolu (1,3-PD) z 3-proc. roztworów wodnych z udziałem aldehydu octowego (aldehyd C2) i izomasłowego (aldehyd izo-C4). Największą efektywność ekstrakcji (97%) uzyskano dla aldehydu izo-C4 zastosowanego w nadmiarze molowym 2:1 w temperaturze pokojowej oraz dla roztworu silnie kwaśnego (pH 0,5). Efektywność aldehydu C2 była dwukrotnie mniejsza. Ekstrakcja reaktywna brzeczek fermentacyjnych zachodziła z nieco mniejszą wydajnością i osiągała 90% w procesie dwustopniowym. W wyniku hydrolizy wydzielonego metodą destylacji reaktywnej acetalu otrzymuje się 1,3-PD oraz aldehyd zawracany do procesu.
EN
1,3-Propanediol (1,3-PD) was extd. from 3% aq. soln. with hexane after acetalization with MeCHO or EtMeCHO. The highest extn. efficiency (97%) was achieved for EtMeCHO in a molar excess of 2:1 at room temp., and for strongly acidic solns. with pH 0.5. The efficiency of MeCHO was twice smaller. The reactive extn. of the fermentation broths occurred to a slightly lower yield of 90% in two-step process. The sepd. acetal was hydrolyzed by reactive distn. to give 1,3-PD and the aldehyde recycled to the process.
10
Content available Group II intron-mediated deletion of lactate dehydrogenase gene in an isolated 1,3-propanediol producer Hafnia alvei AD27
75%
Celińska E. , Drożdżyńska A. , Wita A. , Juzwa W. , Białas W. , Czaczyk K. , Grajek W.
Acta Biochimica Polonica
|
2017
|
tom 64
|
nr 1
123-133
EN
Our previous studies showed that glycerol fermentation by Hafnia alvei AD27 strain was accompanied by formation of high quantities of lactate. The ultimate aim of this work was the elimination of excessive lactate production in the 1,3-propanediol producer cultures. Group II intron-mediated deletion of ldh (lactate dehydrogenase) gene in an environmental isolate of H. alvei AD27 strain was conducted. The effect of the Δldh genotype in H. alvei AD27 strain varied depending on the culture medium applied. Under lower initial glycerol concentration (20 gL-1), lactate and 1,3-propanediol production was fully abolished, and the main carbon flux was directed to ethanol synthesis. On the other hand, at higher initial glycerol concentrations (40 gL-1), 1,3-propanediol and lactate production was recovered in the recombinant strain. The final titers of 1,3-propanediol and ethanol were similar for the recombinant and the WT strains, while the Δldh genotype displayed significantly decreased lactate titer. The by-products profile was altered upon ldh gene deletion, while glycerol utilization and biomass accumulation remained unaltered. As indicated by flow-cytometry analyses, the internal pH was not different for the WT and the recombinant Δldh strains over the culture duration, however, the WT strain was characterized by higher redox potential.
11
Content available Oczyszczanie 1,3-propanodiolu z brzeczki fermentacyjnej z zastosowaniem ekskluzji jonów
75%
Rukowicz B. , Miesiąc I. , Alejski K.
Inżynieria i Aparatura Chemiczna
|
2014
|
tom Nr 4
288--289
PL
W pracy przedstawiono możliwość zastosowania ekskluzji jonów w procesie oczyszczania 1,3-propanodiolu z brzeczki fermentacyjnej. Badania przeprowadzono na kationicie w formie sodowej. Wstępnie odsoloną frakcję produktu uzyskano z ok. 50% wydajnością separacji 1,3-propanodiolu. Stopień odsolenia brzeczki wyniósł 80÷90%, a końcowe oczyszczenie i odbarwienie 1,3-propanodiolu przeprowadzono stosując wymianę jonową. W wyniku procesu otrzymano oczyszczony roztwór 1,3-propanodiolu o stężeniu 16,5 g/L.
EN
Application of ion exclusion for the separation of 1,3-propanediol from fermentation broth was examined. The study was carried out on a cation exchange resin in the sodium form. The product fraction with the desalination degree of 80-90% and 50% separation yield of 1,3-propanediol we obtained. The final purification of 1,3-propanediol was carried out using the ion exchange. As a result of this process the purified solution of 1,3-propanediol (16.5 g/L) was obtained.
12
Content available Influence of reduction time of copper based catalysts: Cu/Al2O3 and CuCr2O4 on hydrogenolysis of glycerol
75%
Wołosiak-Hnat A. , Milchert E. , Lewandowski G. , Grzmil B.
Polish Journal of Chemical Technology
|
2011
|
tom Vol. 13, nr 4
71-76
EN
High activity of copper based catalysts for C-O bond hydro-dehydrogenation and their poor activity for C-C bond cleavage1 have prompted an attempt to apply such catalysts in the hydrogenolysis of glycerol to 1,2- and 1,3-propanediol. In the present study the infl uence of hydrogen reduction time of the Cu/ Al2O3 and CuCr2O4 copper catalysts on glycerol conversion and selectivity of transformation to propanediols and by-products was studied. At fi rst a general comparison was made between the commercial catalysts and those prepared by the co-precipitation method. As better results were obtained in the presence of catalysts prepared by co-precipitation, they were selected for further detailed studies of the influence of reduction time. For both prepared catalysts Cu/Al2O3 and CuCr2O4 the reduction time of 8 h was optimal. In the presence of Cu/Al2O3 catalyst the conversion of glycerol was 59.0%, selectivity of transformation to 1,2-propanediol 77.4% and selectivity to 1,3-propanediol 1.9%. In the presence of CuCr2O4 the glycerol conversion was 30.3% and selectivity to 1,2-propanediol 67.3%.
13
Hypothetical glycerol pathways of newly isolated strains capable of 1,3-propanediol production
75%
Leja K. , Samul D. , Drozdzynska A. , Myszka K. , Juzwa W. , Pawlicka J. , Czaczyk K.
Acta Biochimica Polonica
|
2014
|
tom 61
|
nr 4
14
Content available remote Biotechnological conversion of glycerol from biofuels to 1,3-propanediol using Escherichia coli
75%
Przystałowska H. , Lipiński D. , Słomski R.
Acta Biochimica Polonica
|
2015
|
tom 62
|
nr 1
23-34
EN
In the face of shortage of fossil fuel supplies and climate warming triggered by excessive carbon dioxide emission, alternative resources for chemical industry have gained considerable attention. Renewable resources and their derivatives are of particular interest. Glycerol, which constitutes one of the by-products during biodiesel production, is such a substrate. Thus, generated excess glycerol may become an environmental problem, since it cannot be disposed of in the environment. The most promising products obtained from glycerol are polyols, including 1,3-propanediol, an important substrate in the production of synthetic materials, e.g. polyurethanes, unsaturated polyesters, and epoxy resins. Glycerol can be used as a carbon and energy source for microbial growth in industrial microbiology to produce 1,3-propanediol. This paper is a review of metabolic pathways of native producers and E. coli with the acquired ability to produce the diol via genetic manipulations. Culture conditions during 1,3-PDO production and genetic modifications of E. coli used in order to increase efficiency of glycerol bioconversion are also described in this paper.
15
Content available remote Otrzymywanie 1,3-propanodiolu z glicerolu w bioreaktorze membranowym
75%
Gryta M. , Waszak M. , Morawski A. W.
Przemysł Chemiczny
|
2015
|
tom T. 94, nr 4
628-632
PL
Przedstawiono wyniki badań otrzymywania 1,3-propanodiolu z glicerolu metodą fermentacji z użyciem bakterii Citrobacter freundii. Proces fermentacji prowadzono w bioreaktorze membranowym, w którym zastosowano trójstopniową separację. W pierwszym stopniu stosując mikrofiltrację, uzyskano klarowny filtrat, który następnie rozdzielono metodą nanofiltracji. Otrzymany roztwór 1,3-propanodiolu zatężono w procesie destylacji membranowej. Uzyskany w tym procesie destylat oraz retentat z procesu nanofiltracji zawrócono do bioreaktora, co pozwoliło zamknąć obieg wody oraz odzyskać składniki odżywcze niezbędne do procesu fermentacji.
EN
Glycerol was converted to 1,3-propanediol by fermentation with use of Citrobacter freundii bacteria in a membranę bioreactor with a 3-stage sepn. A clean microfiltrate was sepd. by nanofiltration. The aq. soln. of 1,3-propanediol was concd. by membrane distn. Both the distillate and the retentate from nanofiltration were recycled to the bioreactor to close the H₂O cycle and recover the nutrients.
16
Microbiological conversion of pure and crude glycerol to 1,3-propanediol
75%
Orczyk D. , Szymanowska-Powalowska D. , Leja K.
Acta Scientiarum Polonorum. Biotechnologia
|
2012
|
tom 11
|
nr 3
EN
1,3-propanediol (1,3-PD) is the important product used in chemical industry. Microbiological synthesis of 1,3-PD from crude glycerol is a good solution, both from an economic and environmental point of view. The aim of this work was to investigate the ef­fect of raw material (pure and crude glycerol) on the efficiency of the synthesis of 1,3-PD by the bacteria Clostridium butyricum DSP1 and Clostridium butyricum DO14 isolated from the samples taken from natural environment. Two strains of C. butyricum were simul­taneously investigated. The obtained results showed that the concentration of 1,3-PD was slightly lower in the case of crude glycerol than in pure glycerol, for both strains. Moreover, waste glycerol was not completely utilized.
PL
1,3-propanodiol (1,3-PD) jest ważnym związkiem chemicznym stosowanym w przemyśle chemicznym. Mikrobiologiczna synteza 1,3-PD z wykorzystaniem odpad­owego glicerolu jest atrakcyjnym rozwiązaniem zarówno z ekonomicznego, jak i ekologic­znego punktu widzenia. Celem niniejszej pracy było zbadanie wpływu surowca (czystego i odpadowego glicerolu) na efektywność syntezy 1,3-PD przez bakterie Clostridium butyri­cum DSP1 i DO14. Bakterie wyizolowano ze środowiska naturalnego. Końcowe stężenia 1,3-PD były zbliżone dla obydwu badanych szczepów. W przypadku odpadowych gliceroli stężenie 1,3-PD było nieco niższe niż dla czystego glicerolu. Zastosowanie odpadowych gliceroli nie pozwoliło na całkowitą utylizację glicerolu.
17
Isolation and identification of Clostridium spp. from natural samples that performs effective conversion of glycerol to 1,3-propanediol
63%
Leja K. , Myszka K. , Kubiak P. , Wojciechowska J. , Olejnik-Schmidt A. K. , Czaczyk K. , Grajek W.
Acta Scientiarum Polonorum. Biotechnologia
|
2011
|
tom 10
|
nr 2
EN
Indigenous bacteria in the natural environment can product a wide range of metabolites more efficiently. The aim of this work was to isolate from the natural environment non-pathogenic Clostridium strains that are able to convert glycerol to 1,3-propanediol and other metabolites of potential uses in industry. The effective methods of selection and maintenance of anaerobic cultures in the laboratory conditions were also investigated. Samples were pre-cultured on modified PY medium consisted 50 g/l of glycerol. Isolated colonies growth on TSC medium were screened on the basis of morphological characters typical for Clostridium sp. Isolated bacterial strains were allowed to growth in selective media such as RCM and modified PY. The metabolites of bacteria were investigated by the HPLC technique. The bacteria strains were identified by 16S rRNA technique. The highest percentage of isolates of the genus Clostridium were obtained from excrements of animals, compost, and silages. Nearly 60% were able to convert glycerol to 1,3-propanediol. The highest capacity for utilization of glycerol to 1,3-propanediol was observed in case of the species of Clostridium bifermentans and Clostridium sordelli. The most of examined microflora were also able to short-chain organic acids and ethanol synthesis.
PL
Szereg procesów metabolicznych jest efektywniej przeprowadzanych przez mikroflorę kolonizującą środowisko naturalne. Celem pracy była selekcja niepatogennych kultur bakterii z rodzaju Clostridium ze środowiska naturalnego zdolnych do konwersji glicerolu do 1,3-propanodiolu i innych metabolitów o znaczeniu przemysłowym. Badania dotyczyły także opracowania efektywnych procedur izolacji oraz hodowli mikroorganizmów beztlenowych w warunkach laboratoryjnych. Wstępną adaptację mikroflory obecnej w próbach środowiskowych przeprowadzono na podłożu PY zawierającym glicerol w stężeniu 50 g/l. Hodowle prowadzono w warunkach beztlenowych w anaerostatach. Wyraźnie oddzielone kolonie o morfologii typowej dla Clostridium sp. na podłożu TSC posiewano na podłoża wybiórczo-namnażające RCM oraz PY. Zawartość metabolitów w płynie pohodowlanym oceniano za pomocą techniki HPLC. Identyfikacji gatunkowej dokonano metodą amplifikacji sekwencji kodującej 16S rRNA. Najwyższy odsetek kultur bakteryjnych Clostridium sp. pozyskano z odchodów zwierzęcych, kompostów i obornika. Blisko 60% uzyskanych izolatów wykazywało zdolność syntezy 1,3-propanodiolu z glicerolu. Najwyższą zdolnością do utylizacji glicerolu do 1,3-propanopdiolu charakteryzowały się szczepy Clostridium bifermentans oraz Clostridium sordelli. Większość przebadanych drobnoustrojów wykazywała także zdolność do syntezy krótkołańcuchowych kwasów organicznych i etanolu.
18
1,3-propanediol production by Escherichia coli expressing genes of dha operon from Clostridium butyricum 2CR371.5
63%
Dabrowski S. , Pietrewicz-Kubicz E. , Zablotna E. , Dlugolecka A.
Acta Biochimica Polonica
|
2012
|
tom 59
|
nr 3
EN
 1,3-propanediol is used as a monomer in the production of some polymers e.g. polytrimethylene terephthalate used in the production of carpets and textile fibers and in the thermoplastics engineering. However, the traditional chemical synthesis is expensive, generates some toxic intermediates and requires a reduction step under high hydrogen pressure. Biological production of 1,3-propanediol could be an attractive alternative to the traditional chemical methods. Moreover, crude glycerol which is a by-product of biodiesel production, can be used. We constructed a recombinant Escherichia coli strain producing 1,3-propanediol from glycerol by introducing genes of the dha operon from Clostridium butyricum 2CR371.5, a strain from our collection of environmental samples and strains. The E. coli strain produced 3.7 g of 1,3-propanediol per one litre of culture with the yield of 0.3 g per 1 g of glycerol consumed.
19
Content available Zatężanie roztworów 1,3-propanodiolu metodą destylacji membranowej
63%
Gryta M.
Inżynieria i Aparatura Chemiczna
|
2013
|
tom Nr 5
421--422
PL
Proces destylacji membranowej wykorzystano do zatężania roztworów 1,3-propanodiolu, uzyskując ponad 99,6% zatrzymanie alkoholu w koncentracie. W procesie zastosowano kapilarne membrany Accurel PP. Przeprowadzone długoterminowe badania zatężania (ponad 600 h) wykazały dobrą odporność użytych membran polipropylenowych na zwilżanie.
EN
Membrane distillation process was utilized for the concentration of 1,3-propanediol solutions resulting in the retention of alcohol in retentate by more than 99.6%. Long-term studies of the concentration carried out for over 600 h had demonstrated a good resistance to wettability of used polypropy¬lene Accurel PP membranes.
20
Screening of environmental samples for bacteria producing 1,3-propanediol from glycerol
63%
Dabrowski S. , Zablotna E. , Pietrewicz-Kubicz D. , Dlugolecka A.
Acta Biochimica Polonica
|
2012
|
tom 59
|
nr 3
EN
 Twenty nine environmental samples were screened for the presence of anaerobic microorganisms fermenting glycerol with 1,3-propanediol as a final product. Seven samples were then selected for the next step of our research and eight bacteria strains were cultured anaerobically. Seven of them produced 1,3-propanediol with a yield of 0.47-0.58. Six of the the isolated microorganisms were then classified as Clostridium butyricum (four strains), C. lituseburense (one strain), and C. sartagoforme (one strain). We suggest that of all these strains C. butyricum 2CR371.5 is the best 1,3-propanediol producer as producing no lactate as a by-product and growing well on a glycerol-containing medium.
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