Wyniki wyszukiwania - Biblioteka Nauki

1

Aktualne dane dotyczace syndromu epizootycznego ronienia i zaburzen oddechowych u swin [PRRS]

100%

Pejsak Z. , Truszczynski M.

Medycyna Weterynaryjna

|

1994

|

tom 50

|

nr 03

104-106

2

Trudnosci serologicznej diagnostyki brucelozy bydla

100%

Larski Z.

Medycyna Weterynaryjna

|

1996

|

tom 52

|

nr 12

755-757

3

Dwustopniowa diagnostyka serologiczna boreliozy - weryfikacja metod

100%

Tokarska-Rodak M. , Panczuk A. , Koziol-Montewka M.

Rozprawy Naukowe. Państwowa Wyższa Szkoła Naukowa im. Papieża Jana Pawła II w Białej Podlaskiej

|

2007

|

tom 01

PL

Serologiczna diagnostyka boreliozy mimo opracowania i wprowadzenia różnych metod wciąż wymaga weryfikacji. Metody przesiewowe najczęściej stosowane do oceny przeciwciał anty - Borrelia to metoda jakościowa ELFA (Enzyme Linked Fluorescent Assay, analizator Vidas), metoda immuno - fluorescencji pośredniej (IIFT) oraz metoda immunoenzymatyczna ELISA. Wszystkie wymienione metody charakteryzują się wysoką czułością. Ze względu na możliwość wystąpienia nieswoistych reakcji krzyżowych i wyników fałszywie dodatnich wskazana jest weryfikacja wyników wątpliwych i dodatnich uzyskanych metodami przesiewowymi. Weryfikacji dokonuje się testem Western biot (Wb) o wysokiej swoistości. Technika Wb pozwala na identyfikację przeciwciał dla białek wysoce specyficznych dla Borrelia burgdorferi: VIsE, p83, p39, p31 (OspA), p30, p25 (OspC), p21, p19, p17, wśród których białko VIsE jest najbardziej swoiste dla odpowiedzi w klasie IgG oraz białko OspC, którego obecność związana jest z produkcją przeciwciał IgM. Celem niniejszej pracy była analiza wyników uzyskanych metodą ELFA (z analizatorem Vidas) i metodą IIFT jako testów przesiewowych stosowanych w diagnostyce zakażeń krętkiem B. burgdorferi.

EN

The Lyme borreliosis has become a serious diagnostic problem in Europe and in Poland. The selection of the most specific serologic methods for the diagnosis of Borrelia burgdorferi infections requires some re-evaluation. Screening tests used most frequently for the qualitative detection of anti- Borrelia antibodies include the ELFA (Enzyme Linked Fluorescent Assay; analysis device mini VIDAS) assay whereas the quantitative methods are represented by the indirect immunofluorescence test (IIFT) and ELISA. All the above mentioned methods are characterized by a high sensitivity. Nevertheless, due to a risk of non - specific cross - reactions and false - positive results, verification of doubtful and positive results obtained by screening methods is recommended. The verification can be accomplished by the application of the Western Blot test characterized by a high specificity. The major advantage of the WB technique is identification of proteins, which demonstrate a high specificity for B. burgdorferi: VIsE, p83, p39, p31 (OspA), p30, p25 (OspC), p21, p19, and p17. Among them, the VIsE protein is the most specific protein for IgG antibody production whereas the OspC protein is connected with the presence of IgM antibodies. The aim of the study was the analysis of results obtained by the ELFA (along with the Vidas analyser) and the IIFT methods as screening tests used in the diagnosis of B. burgdorferi infections.

4

Wstępne badania nad przydatnością ELISA w diagnostyce serologicznej gruźlicy bydła w Polsce

86%

Lipiec M. , Krajewska M. , Szulowski K.

Medycyna Weterynaryjna

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2013

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tom 69

|

nr 11

EN

The aim of the study was to perform a preliminary evaluation of the ELISA test for the diagnosis of bovine tuberculosis in Polish conditions. The Idexx kit of immunoassays for detecting the presence of antibodies specific for Mycobacterium bovis in samples of cattle’s blood serum and plasma was used to examine a total of 50 serum samples from clinically healthy animals, 44 sera from cows with previous positive result of tuberculin tests, but negative in microbiological examination, 25 sera from animals from whose tissues strains of Mycobacterium bovis had been isolated (including 10 sera from cows with characteristic tuberculous lesions and 15 sera from animals without these lesions), and 16 serum samples from animals reacting positively for Johne’s disease. On the basis of the results of these examinations, the specificity of the test was determined at 100% and sensitivity at 90%. There were no cross-reactions, and the results indicate that the ELISA Idexx kit may be used as an important diagnostic tool in the diagnosis of bovine tuberculosis.

5

Zastosowanie metody western blot i rekombinowanego bialka p24 wirusa bialaczki bydla w diagnostyce serologicznej

86%

Bicka L. , Kozaczynska B. , Kuzmak J.

Medycyna Weterynaryjna

|

2001

|

tom 57

|

nr 08

586-590

6

Serologiczne rozpoznawanie enzootycznej bialaczki bydla w wojewodztwie torunskim w latach 1990-1997

86%

Meyer-Borowska E. , Krawczyk M.

Życie Weterynaryjne

|

1998

|

tom 73

|

nr 01

24

7

Otrzymywanie przeciwcial monoklonalnych do diagnostyki pomoru klasycznego swin

86%

Markowska-Daniel I. , Pejsak Z.

Medycyna Weterynaryjna

|

1994

|

tom 50

|

nr 04

159-161

EN

Monoclonal antibodies (MAbs) for diagnosis of classical swine fever have been produced. Hog cholera virus, the Alfort strain, was used as an antigen for the immunization of mice; it was propagated in the culture of primary swine kidney cells. Mice of the BALB-c strain were immunized in four cycles and the spleen cells of the mice characterized by the highest titers of antibodies were taken for hybridization. The spleen cells were fused with S p2/o myeloma cells in the presence of PEG 4000. The hybrydoma cells were cultivated in a 20 per cent DMEM HY-HT medium at 37°C with a 5 per cent presence of CO2. The supernatant fluids were tested for the presence of specific antibodies by ELISA and NPLA assays. The selected cultures were subcloned twice in a semi-solid agar, then they were isotyped using the Immuno-Select TM set. Intraperitoneal inoculation of hybri- doma cells male BALB/c mice resulted in the production of ascites fluids containing MAbs. The ascites fluids were purified by means of an Econo-Pac Protein A Kit and conjugated with horse-radish peroxidase.

8

Diagnostyka enzootycznej bialaczki bydla przy uzyciu testu seroneutralizacji

86%

Rulka J. , Buzala E.

Medycyna Weterynaryjna

|

1996

|

tom 52

|

nr 11

708-711

EN

The seroneutralization test based on the inhibition of syncytial effect (SN-ST) turned out to be very useful in the serological diagnostics of enzootic bovine leukaemia. The mean coefficient value of the SN-ST for positive sera (ELISA titer from 200 to 3200) was 0.1, for doubtful (ELISA = 100) - 0.52 and for negative sera 0.94. In case of negative serum the multinuclear cells (syncytium) were very large and numerous and with 85 to 100 nuclei; for doubtful sera they were small, scarce and with 25 to 30 nuclei. No syncytia were observed with the presence of negative sera. The time of incubation of BLV with positive sera played a very important role in the process of seroneutralization. The best results of SN-ST, which corresponded to the ELISA, were found after two hours of neutralization.

9

Serologiczna diagnostyka goraczki Q u bydla i ludzi

86%

Sobiech E. , Dobracki W.

Medycyna Weterynaryjna

|

1996

|

tom 52

|

nr 04

250-252

EN

Sera taken from 449 cows which aborted in years 1991-1994 have been assessed by the complement fixation test for the presence of Q-fever antibodies. It was found that 10.7% of the animals had antibodies against Coxiella burnetii II antigen. Moreover, in the examinations repeated three times on 150 cows coming from a farm in which several cases of abortions were observed, about 15% of seroreagents were noted. At the same time 14 persons working in that farm and additionally 22 humans drinking milk of the cows were tested by the fluorescent antibody test. Specific antibodies against C. burnetii I and II were detected; however lower concentrations of antibodies were found against C. burnetii phase I.

10

Epizootiologia i serodiagnostyka brucelozy bydla w Polsce

86%

Krolak M. , Kolacz J. , Stryszak A. , Pilaszek J.

Życie Weterynaryjne

|

1994

|

tom 69

|

nr 07

257-261

11

Diagnostyka serologiczna enzootycznej bialaczki bydla antygenem BOVINCR

86%

Buzala E. , Rulka J. , Deren W.

Medycyna Weterynaryjna

|

1999

|

tom 55

|

nr 12

823-825

12

Enzootyczna białaczka bydła - problemy z diagnostyką i zwalczaniem

86%

Kuzmak J.

Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii

|

2007

|

tom 02

|

nr 3

13

Zastosowanie testu immunoperoksydazowego w diagnostyce serologicznej rozrodczo-oddechowego zespolu chorobowego swin

86%

Stadejek T. , Pejsak Z.

Medycyna Weterynaryjna

|

1995

|

tom 51

|

nr 06

340-342

EN

The porcine respiratory and reproductive syndrome is a relatively new swine disease first described in the USA in 1987, and in Europe in 1990. Serodiagnosis of the disease can be performed by using an immunoperoxidase mono- layer assay (IPMA), To evaluate this test, the following materials were used to perform the assay: Hesse strain of PRRSV, MARC-145 cells, 42 sera obtained from PRRS suspected herd, 60 sera from herds free from PRRS, and positive reference anti-PRRSV serum. In order to test the sera for the presence of antibodies to PRRSV, 50 pi of successive, 2-fold serum dilutions (from undiluted to diluted 1:256) were distributed in triplicate on the prepared 96-well microplate. The serum samples were considered as positive if brownish-red precipitates were found in the cell cytoplasm of at least one of the tested serum dilutions. The presence of antibodies to PRRSV was found in 23 out 42 examined sera from a suspected farm. In 10 out of 23 positive serum samples the titre was >1:256. No antibodies to PRRSV were detected in the sera that came from swine herds free from PRRS. Taking into account our results, the IPMA can be applied for routine serodiagnosis of PRRS in Poland.

14

Diagnostyka serologiczna parainfluenzy bydła przy użyciu mikrotestu zahamowania hemaglutynacji z krwinkami gęsi

86%

Rulka J.

Medycyna Weterynaryjna

|

1988

|

tom 44

|

nr 09

RU

Отмечено положительное влияние абсорбции неспецифических рецепторов в сыворотках скота при применении 30% суспензии кровяных телец гуся. Абсорбция проводилась в темп. 37°С в течение 30 мин. с использованием 3,1X10⁷—9,2Х10⁷ эритроцитов/0,7 мл сыворотки. Добавка телец к усследуемой сыворотке в отношении 1:10, не влияет отрицательно на титр противотел для виртса РI₃ в микротесте HI. Результаты теста, проведенного на 3 плитках, указывают на повторяемость полученных результатов. Неабcoрбирoванные сыворотки, дающие отрицательный результат тесте, после абсорбции показывали титр 1:10—1:40±. Проведенные исследования указывают на полную пригодность кровяных телец гуся и плиток фирмы PLASTOMED к кутиновой серологической диагностике параинфлюэнцы скота.

EN

A profitable influence of non-specific absorption of inhibitors from bovine sera by means of a 30 per cent suspension of goose erythrocytes was found. The process of absorption was carried out at 37°C for 30 minutes using 3,1X10⁷—9,2Х10⁷ erythrocytes per 0.7 ml of serum. The addition of erythrocytes to the serum in relation 1:10 did not influence the level of antibodies against PI-3. The results of the microtest were reproducible. Non-absorbed sera reacting negatively following absorption had titres from 1:10 to 1:40. The examinations point to the usefulness of goose erythrocytes and plates, produced by Plastomed, for serological tests performed with bovine parainfluenza virus.

15

Ocena polskiego zestawu ELISA w serologicznej diagnostyce bydla zakazonego wirusem bialaczki [BLV]

86%

Rulka J. , Lis M. , Buzala E.

Medycyna Weterynaryjna

|

2000

|

tom 56

|

nr 10

660-664

16

Charakterystyka mutantow genetycznych wirusa enzootycznej bialaczki bydla i ich znaczenie w diagnostyce serologicznej

86%

Bicka L. , Rola M. , Kozaczynska B. , Stec J. , Kuzmak J.

Medycyna Weterynaryjna

|

2005

|

tom 61

|

nr 06

699-702

17

Ocena przydatnosci wybranych antygenow Mycoplasma pneumoniae do serodiagnostyki mykoplazmozy

86%

Rastawicki W. , Rokosz N. , Jagielski M.

Medycyna Doświadczalna i Mikrobiologia

|

2009

|

tom 61

|

nr 2

175-182

18

Enzootyczna bialaczka bydla

86%

Bartoszkiewicz J.

Zielonogórski Informator Rolniczy

|

1995

|

nr 06

8-9

19

Ocena testu ELISA do wykrywania przeciwcial dla wirusa BHV 1

72%

Rola J. , Zmudzinski J.

Medycyna Weterynaryjna

|

1997

|

tom 53

|

nr 03

148-150

20

Ocena efektywnosci diagnostyki testem ELISA zakazen wywolywanych przez Mycoplasma pneumoniae w Polsce, w latach 2006-2008

72%

Kaluzewski S. , Rastawicki W. , Namysl E.

Medycyna Doświadczalna i Mikrobiologia

|

2009

|

tom 61

|

nr 2

183-190