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Content available remote Fibrinogen and antithrombin in hematological patients with neutropenic fever
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The value of plasma fibrinogen and antithrombin as predictors of severe sepsis was investigated in 69 adult hematological patients, who had altogether 93 periods of neutropenic fever. Patients had either acute myeloid leukemia or had received a high dose of chemotherapy supported by autologous stem cell transplantation. In febrile periods with severe sepsis, the median fibrinogen concentration at the start of the fever was significantly higher (5.0 g/L) than that without severe sepsis (4.5 g/L) (p=0.009). Normal plasma fibrinogen could rule out a group of patients with severe sepsis at the beginning of the fever. The antithrombin activity decreased, both in fever periods with severe sepsis and in those without. The decrease in antithrombin activity was found to be greater in fever periods characterized by severe sepsis. In conclusion, elevated plasma fibrinogen and constantly decreasing antithrombin were shown to be linked to the development of severe sepsis.
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Content available remote Interaction of fibrinogen with nanosilica
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Interaction of human plasma fibrinogen (HPF) with fumed nanosilica A-300 in a phosphate buffer solution (PBS) was studied using 1H NMR spectroscopy with layer-by-layer freezing-out of bulk and interfacial water in the temperature range of 210–273 K, TSDC (90 T FTIR, and UV spectroscopy methods. An increase in concentration of HPF in the PBS leads to a decrease in amounts of structured water (frozen at T FTIR and UV spectra show that the HPF adsorption on silica leads to structural changes of the protein molecules. These changes and formation of hybrid HPF/A-300 aggregates can increase the rate of clotting that is of importance on nanosilica application as a component of tourniquet preparations. [...]
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