Wyniki wyszukiwania - Biblioteka Nauki

1

Expression and polymorphism of defensins in farm animals

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Bagnicka E. , Strzałkowska N. , Jóźwik A. , Krzyżewski J. , Horbańczuk J. , Zwierzchowski L.

Acta Biochimica Polonica

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2010

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tom 57

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nr 4

487-497

EN

Due to their activity against bacteria, viruses, and fungi, antimicrobial peptides are important factors in the innate resistance system of humans and animals. They are called "new generation antibiotics" for their potential use in preventive and therapeutic medicine. The most numerous group of antimicrobial peptides is a family of cationic peptides which include defensins and cathelicidins. Among them the most common are peptides with a beta-sheet structure containing three intra-molecular disulphide bonds, called defensins, comprising three classes: alpha, beta, and theta. The class of beta-defensins is the largest one. Their transcripts have been found in many tissues of humans and animals. The aim of this paper is to present the current knowledge about antimicrobial peptides from the defensin family in farm animals, their expression, polymorphism, as well as the potential of their use as genetic markers of health and production traits.

2

MUZYKA I MORALNOŚĆ: Ekspresja i katharsis w symfoniach Piotra Czajkowskiego

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Szerszenowicz J.

Miscellanea Anthropologica et Sociologica

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2015

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tom 16(3)

94-114

PL

Paradoksalne powiedzenie, że Czajkowski skomponował trzy Symfonie, IV, V i VI, odzwierciedlasytuację całkowitej nieobecności na estradach koncertowych trzech pierwszychSymfonii, podczas gdy trzy ostatnie biją rekordy popularności. Wydaje się, że sekretich powodzenia leży w ogromnym ładunku emocjonalnym porywającym słuchaczy, cooczywiście rodzi pytanie o pochodzenie tego trwałego, choć wcześniej nieobecnego, ładunku.Niebywałe napięcie i dramatyzm kompozycji podsuwają hipotezę, że Symfoniajest obrazem wewnętrznego rozdarcia, którego źródłem był freudowski konflikt międzynaturą i kulturą na tle orientacji homoseksualnej. Nie był to konflikt utajony – zepchnięty,jak u Leonarda da Vinci, w podświadomość i objawiający się poprzez zamaskowane symbole.Czajkowski był w pełni świadomy, iż jego skłonności seksualne są społecznie nie dozaakceptowania i stanowią zagrożenie tak dla niego samego, jak i dla najbliższych. Próbystłumienia czy choćby zamaskowania tych skłonności okazały się jednak bezowocne. Cowięcej – w dramatyczny sposób ukazywały beznadziejność starań i pogłębiały depresję.

3

Obraz zaburzeń emocjonalnych w autonarracji K.R. Jamison

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Gawda B. , Kosacka K.

Zagadnienia Rodzajów Literackich

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2017

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tom 60

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nr 3 (123)

95-109

EN

The aim of this work is to reconstruct the picture of emotional disorders as revealed in Jamison’s self-narration. The starting point was the question whether self-narration can be a source of information about the patterns of experience of disorder. The self-narrations expressed in the book An Unquiet Mind have been analyzed. The picture of emotional disorders has been reconstructed including the course of depressive and manic episodes and their alternations. The Jamison’s personal description of the experience of disorder was a valuable record of the perception of her own and the world.

4

Reactive oxygen species upregulate expression of PAI-1 in endothelial cells

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Swiatkowska M. , Szemraj J. , Al-Nedawi K. N. I. , Pawlowska Z.

Cellular and Molecular Biology Letters

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2002

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tom 07

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nr 4

EN

Second messengers involved in the signal transduction pathway leading to induction of the plasminogen activator inhibitor (PAI-1) have not yet been well characterized. This study focuses on the mechanisms of regulation of PAI-1 expression by reactive oxygen species (ROS) in human endothelial cells. Inhibition of the tumor necrosis factor α (TNFα)-induced expression of PAI-1 by antioxidant N-acetyl-L-cysteine (NAC) indicated redox-sensitive mechanisms involved in the signalling pathway. Because TNFα induces PAI-1 production in endothelial cells, and NAC attenuated this response, we attempted to investigate the possible involvement of ROS in the activation of PAI-1 by TNFα. Upregulation of PAI-1 expression in endothelial cells by the stimulation with TNFα (50ng/ml) or H2O2 (10-200µM), observed by measurement of the antigen and mRNA levels, was reversed in the presence of NAC (20mM). The stimulatory effect of ROS was detected also at the level of the PAI-1 promoter in endothelial cells transfected with plasmid p800 LUC containing a PAI-1 promoter fragment (+71 to -800). The PAI-1 promoter activity was increased in the presence of ROS, and was suppressed by up to 75% in the presence of antioxidants [1], On the basis of this study we can conclude that reactive oxygen species play an important role in a cytokine-induced activation of PAI-1 expression, and may act as a signal transduction messenger.

5

Overview of the KTH rule system for musical performance

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Friberg A. , Bresin R. , Sundberg J.

Advances in Cognitive Psychology

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2006

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tom 2

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nr 2-3

145-161

EN

The KTH rule system models performance principles used by musicians when performing a musical score, within the realm of Western classical, jazz and popular music. An overview is given of the major rules involving phrasing, micro-level timing, metrical patterns and grooves, articulation, tonal tension, intonation, ensemble timing, and performance noise. By using selections of rules and rule quantities, semantic descriptions such as emotional expressions can be modeled. A recent real-time implementation provides the means for controlling the expressive character of the music. The communicative purpose and meaning of the resulting performance variations are discussed as well as limitations and future improvements.

6

Funkcjonalizm w architekturze: od neolitu do wczesnego modernizmu

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Tarnowski J.

Estetyka i Krytyka

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2009

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nr 15-16

190-213

EN

In line with modernist historiography, functionalism was construed as the foundation of modern architecture. The exact meaning of the concept in the modernist theory of architecture, however, is unclear. If functionalism is to be understood, after Adler, Mies and Sullivan’s dictum that „form follows function,” as the outward expression of function in form and/or mere functionality, functionalism no longer appears to be restricted to modernism: functionalist buildings can be shown to have been erected in all ages from as far back as the Neolithic beginnings of architecture. The actual difference between modern and traditional architecture, therefore, is to be found not in the greater functionality of the former or in its clearer manifestation of function, but rather in form itself. Modern architecture is a radical move away from the formal tradition of classical architecture. (To be continued in Volume 18)

7

MUSIC AND MORALITY: Expression and Katharsis in Peter Tchaikovsky’s symphonies

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Szerszenowicz J.

Miscellanea Anthropologica et Sociologica

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2015

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tom 16(3)

94-114

EN

The most fascinating task of aesthetics is to detect the hidden order of arts. As a matterof fact, the last three symphonies of Tchaikovsky received an enthusiastic reception becauseof their unusually strong expression and energy. So far, however, we know neitherwhy his previous symphonies had lacked such power, nor why each one of the last threeis so impressive. It seems that thanks to the psychoanalytical approach we can make itclear that the source of these qualities lies in the conflict between the composer’s homosexualpreferences and the moral norms of his time. The psychical tension between Idand Superego evoked the feeling of guilt which entailed the composer’s fight against hissusceptibility. Ultimately, the unsuccessful marriage disclosed the hopelessness of his efforts,putting the composer into a poor mental condition in which the symphony no. 4op. 36 came into existence. In a letter to his patron Mrs. von Meck Tchaikovsky describedan objective “program” of the symphony. He sketched the plan of a drama: the motif offate which “hangs over the head like the sword of Damocles”, the state of depression, andglimmers of hope… This description suits well both the sound flow of the symphony andthe course of the composer’s life. Although the next two symphonies were not describedby the composer in such manner, a lot of features analogous to the op. 36 are noticeable.Therefore, the same key can be applied to the interpretation of their dramatic contents.The first movement of each symphony begins with the introduction based on the characteristicmotif of fate which comes and goes as the music develops. The music itselfoscillates between tension and fading, only to explode in tutti with great strength andthickening texture of sounds, and with obsessive motifs. Further, the energy decreasesand the music falls down into low, dark regions. It dies away – but then again, it cheersup and achieves the greatest tension.In the biography of Tchaikovsky there is a great number of facts explaining the significantdifferences between the three symphonies. For instance, the symphony no. 5manifests temporal reconciliation with fate by the brighter mood and a particular wayof elaborating the motif of fate. On the other hand, the generally depressive mood of thesymphony no. 6 (Pathétique) reflects the deterioration of the composer’s physical and psychologicalcondition. It seems that the self-expression ceased to play the role of the catharsis– the purification of mind from bad tensions. There has been a controversy about thecause of Tchaikovsky’s death which occurred briefly after the completion of the symphonyPathétique. The well grounded hypothesis suggesting the composer’s suicide is based onthe same facts which determined the contents of the symphony. Music happens to be themirror of life and the intuition of death.

8

Wolność słowa vs. wolność religijna. Wybrane zagadnienia

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Biłgorajski A.

Studia Politologiczne

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2012

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tom 23

95-120

EN

It seems that freedoms: freedom of speech and religious liberty, should be treated comprehensively and complementarily. Comprehensively – because complete fruition one of them implicates existing the other one. Complementarily – because the freedoms are interrelated/connected in such way that they could encourage/ support and limit each other. Obvious implication in this matter is frequent crossing of conflicts as well as connecting mentioned liberties (for example secular interests with sects interests, religious liberty with ideas concerning wounding of religious feelings; belief liberty with wounding of belief liberty through demonstration of opponents). On the one hand how the freedom of speech is pledge of secular state, on the other hand how the freedom of speech might be risk for it? I will try to answer this question, consisting in decisions of USA Supreme Court and European Court of Human Rights in Strasbourg.

9

Molecular cloning and characterization of a peroxiredoxin from Phanerochaete chrysosporium

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Jiang Q. , Yan Y. H. , Hu G. K. , Zhang Y. Z.

Cellular and Molecular Biology Letters

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2005

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tom 10

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nr 4

10

„Otwieramy uczniom oczy”. Innowacyjne działania dydaktyczne

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Kiwacka A. , Letkiewicz-Zamora J.

Paidia i Literatura

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2020

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nr 2

173-182

EN

The article consists of two parts: the first one includes the introduction to art therapy theory, the second one indicates the possibilities of its application in youth education. Agata Kiwacka and Joanna Letkiewicz‑Zamora - with over 20 years of experience in teaching the Polish language, they both work in integration classes in the Middle School No. 1 with Integration Departments in Bytom. They are also special educators in typhlopedagogy and art therapy. They have been using elements of art therapy during Polish classes. They want to expand their work, so they wrote their own programme, which was deployed in the integration classes in the middle school. They share their experiences from the past four years. They are continuously improving, so that the work they have taken on, would continue to give even more to the young people. They help the pupils get to know and accept themselves. They want the pupils to be aware of their worth, open-minded, and creative. So that in the future, they can become “different people.” This change in the young people is happening right in front of their eyes.

11

Collingwood and ‘Art Proper’: From Idealism to Consistency

88%

Dönmez D.

Estetika: The Central European Journal of Aesthetics

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2015

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tom 52

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nr 2

152-164

EN

Collingwood’s ‘art-proper’ definition has been controversial. Wollheim argues that his Theory of Imagination assumes that the nature of the artwork exists solely in the mind, committing him to the Ideal Theory. Consequently, when Collingwood states that the audience is essential for the artist and the artwork, he is being inconsistent. In contrast, Ridley claims that Collingwood’s Expression Theory saves him from Wollheim’s accusations; hence he is consistent and does not support the Ideal Theory. I demonstrate that Collingwood both adheres to the Ideal Theory (contrary to Ridley) and is consistent in his art theory (contrary to Wollheim). I show that imagination is the sufficient condition of art and expression is the process of art’s coming into existence. I argue that Collingwood is consistent in his theory because the audience and the externalization of the work are needed for an appreciation and understanding of the artwork as either good or bad, as either a work of a corrupt consciousness or not. Hence, an account of the role of externalization is a contribution to the epistemology, not the ontology, of art.

12

Regulatory mechanisms for the expression and activity of platelet-derived growth factor receptor.

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Funa K. , Uramoto H.

Acta Biochimica Polonica

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2003

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tom 50

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nr 3

647-658

EN

PDGF is one of the most potent serum mitogens, and the signalling mechanism by way of its receptor tyrosine-kinase has been extensively studied since its first purification in 1979. The identification of homology between the simian sarcoma virus oncogene, v-sis, and the B-chain of PDGF, as well as the frequent over-expression of both the ligands and receptors in various tumours and stroma led to the proposal of the PDGF-mediated autocrine and paracrine hypothesis. Consistent with the important roles of PDGF in the growth and survival of cells, the expression and activity of PDGF receptors are tightly controlled by both positive and negative feedback mechanisms at different levels. The deregulation of the control system can result in serious pathological conditions such as chronic inflammation and tumours. Understanding the molecular mechanisms for the regulatory system and the signalling pathway of PDGF is essential in order to find effective therapies in the diseases where PDGF is involved.

13

Identification and properties of the Deinococcus grandis and Deinococcus proteolyticus single-stranded DNA binding proteins (SSB)

88%

Filipkowski P. , Kur J.

Acta Biochimica Polonica

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2007

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tom 54

|

nr 1

79-87

EN

To study the biochemical properties of SSB's from Deinococcus grandis (DgrSSB) and Deinococcus proteolyticus (DprSSB), we have cloned the ssb genes obtained by PCR and have developed Escherichia coli overexpression systems. The genes consist of an open reading frame of 891 (DgrSSB) and 876 (DprSSB) nucleotides encoding proteins of 296 and 291 amino acids with a calculated molecular mass of 32.29 and 31.33 kDa, respectively. The amino-acid sequence of DgrSSB exhibits 45%, 44% and 82% identity and the amino-acid sequence of DprSSB reveals 43%, 43% and 69% identity with Thermus aquaticus (TaqSSB), Thermus thermophilus (TthSSB) and Deinococcus radiodurans SSBs, respectively. We show that DgrSSB and DprSSB are similar to Thermus/Deinococcus SSBs in their biochemical properties. They are functional as homodimers, with each monomer encoding two single-stranded DNA binding domains (OB-folds). In fluorescence titrations with poly(dT), both proteins bind single-stranded DNA with a binding site size of about 33 nt per homodimer. In a complementation assay in E. coli, DgrSSB and DprSSB took over the in vivo function of EcoSSB. Thermostability with half-lives of about 1 min at 65-67.5°C make DgrSSB and DprSSB similar to the known SSB of Deinococcus radiodurans (DraSSB).

14

ADH expression in aerial-aquatic plants in response to different water environment

88%

Kordyum E. , Kozeko L. , Ovcharenko Y.

Acta Physiologiae Plantarum

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2007

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tom 29

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nr 1 Suppl.

15

Cut Points in PEG

88%

Redziejowski R. R.

Fundamenta Informaticae

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2016

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tom Vol. 143, nr 1/2

141--149

EN

Parsing Expression Grammar (PEG) encodes a recursive-descent parser with limited backtracking. It has been recently noticed that in the situation when the parser is to explore several alternatives one after another, no further alternatives need to be explored after the parser reached certain ”cut point”. This fact can be used to save both processing time and storage. The subject of the paper is identification of cut points, which can also help in producing better diagnostics.

16

An Accent-Based Approach to Automatic Rendering of Piano Performance: Preliminary Auditory Evaluation

88%

Bisesi E. , Parncutt R.

Archives of Acoustics

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2011

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tom Vol. 36, No. 2

283-296

EN

We are exploring the relationship between accents and expression in piano performance. Accents are local events that attract a listener’s attention and are either evident from the score (immanent) or added by the performer (performed). Immanent accents are associated with grouping (phrasing), metre, melody and harmony. In piano music, performed accents involve changes in timing, dynamics, articulation, and pedalling; they vary in amplitude, form, and duration. We analyzed the first eight bars of Chopin Prelude op. 28 n. 6. In a separate study, music theorists had marked grouping, melodic and harmonic accents on the score and estimated the importance (salience) of each. Here, we mathematically modeled timing and dynamics in the prelude in two ways using Director Musices (DM) – a software package for automatic rendering of expressive performance. The first rendering focused on phrasing following existing and tested procedures in DM. The second focused on accents – timing and dynamics in the vicinity of the accents identified by the theorists. In an informal listening test, 10 out of 12 participants (5 of 6 musicians and 5 of 6 non-musicians) preferred the accent-based formulation, and several stated that it had more variation of timing and dynamics from one phrase to the next.

17

Inhibition of CYP17 expression by adrenal androgens and transforming growth factor β in adrenocortical cells.

88%

Biernacka-Łukanty J. M. , Lehmann T. P. , Trzeciak W. H.

Acta Biochimica Polonica

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2004

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tom 51

|

nr 4

907-917

EN

Cytochrome P450c17, encoded by the CYP17 gene, is a component of the 17a-hydroxylase/17,20-lyase enzyme complex essential for production of adrenal glucocorticoids and androgens as well as gonadal androgens. The expression of CYP17 in adrenocortical cells is stimulated by corticotropin (ACTH) via the signal transduction pathway involving cAMP and protein kinase A (PKA). Thus, in addition to glucocorticoids, ACTH stimulates formation of adrenal androgens, which are known to induce transforming growth factor β (TGF-β) secretion. TGF-β in turn inhibits steroid hormone output by attenuating both basal and ACTH-dependent expression of CYP17. The present study revealed that treatment of bovine and human H295R adrenocortical cells with androgens resulted in a decrease in the basal level of CYP17 transcript and cortisol secretion, without affecting forskolin-stimulated levels. We also demonstrated that in H295R cells TGF-β inhibited both basal and forskolin-stimulated accumulation of CYP17 mRNA. Determination of promoter activity, directing luciferase reporter gene expression in H295R cells transfected with deletion fragments of bovine CYP17 promoter, indicated that the -483 to -433 bp fragment of the promoter was necessary for the inhibitory action of TGF-β on CYP17 expression. It is concluded that in bovine and human adrenocortical cells, androgens inhibit basal CYP17 expression probably at the transcriptional level and independently of the effect of TGF-β.

18

Characterisation and molecular dynamic simulations of J15 asparaginase from Photobacterium sp. strain J15

88%

Yaacob M. , Hasan W. , Ali M. , Rahman R. , Salleh A. , Basri M. , Leow T.

Acta Biochimica Polonica

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2014

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tom 61

|

nr 4

745-752

EN

Genome mining revealed a 1011 nucleotide-long fragment encoding a type I l-asparaginase (J15 asparaginase) from the halo-tolerant Photobacterium sp. strain J15. The gene was overexpressed in pET-32b (+) vector in E. coli strain Rosetta-gami B (DE3) pLysS and purified using two-step chromatographic methods: Ni2+-Sepharose affinity chromatography and Q-Sepharose anion exchange chromatography. The final specific activity and yield of the enzyme achieved from these steps were 20 U/mg and 49.2%, respectively. The functional dimeric form of J15-asparaginase was characterised with a molecular weight of ~70 kDa. The optimum temperature and pH were 25°C and pH 7.0, respectively. This protein was stable in the presence of 1 mM Ni2+ and Mg2+, but it was inhibited by Mn2+, Fe3+ and Zn2+ at the same concentration. J15 asparaginase actively hydrolysed its native substrate, l-asparagine, but had low activity towards l-glutamine. The melting temperature of J15 asparaginase was ~51°C, which was determined using denatured protein analysis of CD spectra. The Km, Kcat, Kcat/Km of J15 asparaginase were 0.76 mM, 3.2 s-1, and 4.21 s-1 mM-1, respectively. Conformational changes of the J15 asparaginase 3D structure at different temperatures (25°C, 45°C, and 65°C) were analysed using Molecular Dynamic simulations. From the analysis, residues Tyr24, His22, Gly23, Val25 and Pro26 may be directly involved in the 'open' and 'closed' lid-loop conformation, facilitating the conversion of substrates during enzymatic reactions. The properties of J15 asparaginase, which can work at physiological pH and has low glutaminase activity, suggest that this could be a good candidate for reducing toxic effects during cancer treatment.

19

Funkcja ekspresywna leksyki z zakresu medycyny w listach prywatnych Henryka Sienkiewicza

88%

Mariak L.

Poznańskie Studia Polonistyczne. Seria Językoznawcza

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2014

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tom 21

|

nr 2

53-70

PL

This article contains an analysis of medical lexis conducted in connection with the expressive function performed by this verbal layer.The collected lexical and stylistic material includes expressions and phrases with expressively characterized adjectives and adverbs, expressive and diminutive derivatives, compounds, different stylistic means (e.g., metaphors, comparisons, euphemisms and other).

20

Heterologous expression and initial characterization of recombinant RbcX protein from Thermosynechococcus elongatus BP-1 and the role of RbcX in RuBisCO assembly

88%

Tarnawski M. , Gubernator B. , Kolesinski P. , Szczepaniak A.

Acta Biochimica Polonica

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2008

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tom 55

|

nr 4

777-785

EN

In the cyanobacterial RuBisCO operon from Thermosynechococcus elongatus the rbcX gene is juxtaposed and cotranscribed with the rbcL and rbcS genes which encode large and small RuBisCO subunits, respectively. It has been suggested that the rbcX position is not random and that the RbcX protein could be a chaperone for RuBisCO. In this study, the RbcX protein from T. elongatus was overexpressed, purified and preliminary functional studies were conducted. The recombinant protein purified from Escherichia coli extracts was predominantly present in a soluble fraction in a dimeric form. Coexpression experiments have demonstrated that RbcX can mediate RbcL dimer (L2) formation, and that it is essential for the L8 core complex assembly. This is the first characterization of the RbcX protein from a thermophilic organism.