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PL
Oceniano aktywność fagocytarną związaną z wybuchem tlenowym granulocytów obojętnochłonnych u chorych z boreliozą z Lyme. Wykazano spadek wartości badanych parametrów w ostrej fazie choroby oraz ich normalizację w badaniu odległym, wykonanym po sześciu miesiącach od zakończenia leczenia. Poprawa badanych funkcji neutrofilów w badaniach odległych sugeruje, że obserwowane zmiany mają charakter nabyty, uwarunkowany obecnością krętka Borrelia burgdorferi.
EN
In recent years in Poland, the interest has increased in studies about tick borne diseases, mainly Lyme borreliosis. Immune response and genotype of pathogen play an important role in the course of this disease. Phagocytic cells, especially PMN are dominant in defence mechanisms against bacterial infections. The main feature of PMN is their ability to destroy pathogenic microorganisms by phagocytosis. The aim of this study was to estimate the phagocytic activity of PMN connected with intracellular respiratory burst in patients with Lyme borreliosis. The PMN activity tests completed were: phagocytosis, spontaneous and reducted of nitrotetralizate blue test (NBT). Decreased phagocytic activity and oxygen metabolism of PMN from patients with borreliosis in comparison with values of controls were found. Normalization of these parameters after treatment was observed. Changed phagocytic activity connected with intracellular oxygen metabolism during the course of therapy was the main observation. Depression of phagocytic activity of PMN connected with oxygen metabolism can influence defence reactions in patients with Lyme borreliosis. It is suggested that changes observed are acquired and associated with Borrelia burgdorferi presence.
EN
The purpose of the study was to assess the condition of peripheral blood neutrophiles in horses through using the luminal-enhanced chemiluminescence (CL) method. The study population consisted of 33 clinically healthy horses of both sexes (aged 3-15 years). The chemiluminescence of non-stimulated neutrophiles was measured as well as of those stimulated by a receptor stimulus - myosin (Z), opsonic zymosan (OZ) and formyl-Met-Leu-Phe (fMLP) and an extrareceptor stimulus - phorbol myristate acete (PMA). The results of the study demonstrated that the chemiluminescence of neutrophiles stimulated by Z and OZ was considerably higher in comparison to the values of CL stimulated by fMLP and PMA. The use of stimulators can trigger a "respiratory burst" and may be helpful in assessing the phagocyte activity of equine neutrophiles.
PL
Szczepy S. aureus w zależności od źródła izolacji różnią się opornością na bakteriobójczą aktywność fagocytów - szczepy izolowane z krwi wykazują największą, a szczepy izolowane z dróg oddechowych najmniejszą podatność na zabijanie. Badane szczepy były także zróżnicowane pod względem zdolności do indukcji chemiluminescencji (CL) w monocytach. Nie stwierdzono natomiast różnic w toksyczności badanych drobnoustrojów wobec komórek fagocytujących.
EN
The aim of this study was to investigate the interactions occurring between peripheral blood phagocytes and strains of S. aureus isolated from different clinical specimens (blood, respiratory tract, pus). To evaluate the sensitivity of microorganisms to bactericidal activity of phagocytes, monocytes and granulocytes separated from peripheral blood by standard density gradient and by counter-current centrifugal evaluation these cells were incubated with suspensions of opsonized bacteria. In paralell, the viability of phagocytes was examined by flow cytometry, and the ability of bacteria to trigger reactive oxygen intermediates (ROI) production was evaluated by chemiluminescence measurement. To investigate the efficiency of phagocytosis, bacteria were labelled with fluorescein isothiocynate (FITC) and the percentage of cells containing FITC-labelled bacteria were analysed by flow cytometry. The data obtained show the strains of S. aureus derived from different clinical specimens, differ in their sensitivity to bactericidal activity of phagocytes - strains isolated from the blood show the highest, but strains isolated from the respiratory tract have the lowest sensitivity to killing. These strains differ too in their ability to trigger monocyte CL response. On the contrary, there was no difference in toxicity of bacteria against phagocytes. Strains isolated from peripheral blood showed a significant negative correlation between the ability to trigger CL response and toxicity against phagocytes.
EN
The aim of the study was to assess oxidation-reduction processes in peripheral blood of adolescents with decreased motor activity. Motor activity was estimated in a group of subjects, aged 15 - 18 years. Exemption from physical education classes (PE) longer than 6 months was treated as low motor activity in the estimation of motor activity. Decreased motor activity /↓MA/ was observed in 27 cases, whereas normal motor activity was found in 42 subjects. The following evaluations were performed: lipid peroxidation process - malondialdehyde concentration in erythrocytes /MDAe/ and in plasma /MDAp/; antioxidative enzymatic system - superoxide dismutase activity /SOD/, catalase activity /CAT/ in erythrocytes. Very beneficial changes were observed after individual consideration of all exemptions from PE classes which, as it appeared, had not always been justified. Re-evaluation of physical activity demonstrated a marked increase of the group of physically active adolescents in the investigated population. Beneficial effect - 24,6% of girls and boys became physically active. The obtained results of investigations point to the weakening of antioxidative protection in adolescents with ↓MA as compared to adolescents with normal MA. Higher concentrations of MDAe and MDAp in adolescents with ↓MA prove intensified lipid peroxidation.
EN
The reactivity of rat blood leukocytes after recurrent blood losses was examined. The blood samples were collected from the heart, three times in seven-day intervals. The volume of each sample was approximately 15% of the total blood volume. The functional changes in leukocytes were determined utilizing a test of radial segmentation of nuclei (RS) in mononuclear leukocytes and a test of Saccharomyces cerevisiae yeast phagocytosis. Our results demonstrate that sequential blood loss induced a decrease in the number of mononuclear cells indicating RS from 21.2% after 1st blood sampling up to 13% and 14% in following samplings: a decrease in number of phagocytic granulocytes from 49.5% after 1st blood sampling up to 41% and 39.3% after 2nd and 3rd sampling, respectively; and an increase in the number of phagocytic mononuclear blood cells from 8.5% after the 1st sampling up to 9.2% and 12.7% after the 2nd and 3rd blood samplings respectively. We affirm that this frequent blood loss modified the reactivity of blood leukocytes but did not change the WBC quantity in blood.
EN
Five heifers and five bulls averaging 11 months of age were used to characterise plasma growth hormone (GH) pattern in Polish Friesian cattle. Animals were fed a diet of corn silage, hay and concentrates twice daily. They gained weight at a mean rate of 0.7 kg/d for heifers and 0.9 kg/d for bulls. Serial blood samples were collected through catheters at 15 min intervals over 4 hours. To estimate the pulse, characteristics of GH the computer program PULSAR was used. The results of the study confirm the pulsatile nature of growth hormone secretion in cattle. The following values for plasma GH parameters were obtained for heifers and bulls respectively: mean concentration, 17.8 and 18.7 ng/ml; smooth value, 9.5 and 9.6 ng/ml; peak amplitude, 15.7 and 20.8 ng/ml; peak frequency, 0.98 and 1.02 h⁻¹. The GH sectretion particularly varied among animals for mean and smooth value. A correlation between basic GH concentration and peak amplitude was found. The obtained results suggest that the peak frequency is a biologically important parameter associated with the growth rate.
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