Wyniki wyszukiwania - Biblioteka Nauki

1

Analysis of risk factors of positive peritoneal cytology in patients treated for gastric cancer – preliminary report

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Lisiecki R. , Spychała A. , Peter K. , Murawa D.

Polish Journal of Surgery

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2015

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tom 87(10)

506-512

EN

Presence of free gastric cancer cells in the peritoneal cavity of patients who underwent surgical treatment for gastric cancer is a negative prognostic factor and caused rapid disease recurrence, manifested as peritoneal metastases. Positive peritoneal cytology despite lack of visible peritoneal metastases was regarded as M1 class in the TNM classification (7th edition) in 2010. The aim of the study was to analyze factors associated with positive peritoneal cytology and identify groups of patients in whom diagnostic laparoscopy plus peritoneal lavage in the diagnostic process could affect therapeutic decisions. Material and methods. The study enrolled patients with gastric cancer who underwent surgical treatment at the Department of Surgery, Wielkopolskie Oncology Center in Poznań. During the laparotomy, after opening of the peritoneal cavity, 200 ml of physiological saline at 37°C was administered in the tumor region. After this fluid was mixed, 100 ml of lavage fluid was collected. This fluid was subsequently spun many times to obtain sediment for cytology and immunohistochemistry investigation using anti-BerEp-4, CK 7/20, and B72.3. Results of peritoneal cytology were analyzed jointly with clinical factors – patient’s age, sex and pathology factors – tumor invasion, involvement of lymph nodes, histological grade, histological type according to Lauren and localization of the cancer in the stomach. Results. Analysis of the peritoneal fluid for presence of free cancer cells was done in 51 patients. Positive peritoneal cytology was found in 12 (23.5%) patients. In the group of patients with positive cytology, all patients had T3/T4 tumors and all were found to have lymph node metastases, while G3 cancer was found in 83.3% of patients. In patients with positive cytology, diffuse gastric cancer according to Lauren predominated (9 of 12 patients, 75%), while in patients with negative cytology – intestinal type (20 of 39 patients, 51.2%). In the group of patients with positive histology, the whole stomach was involved by the cancer process in 7 of 12 patients (58.3%), while in the group with negative histology, in 29 of 39 patients the tumor was located in the gastric body and prepyloric part (74.4%). Conclusions. Based on this study we can conclude that determinants of positive peritoneal cytology include: tumor stage T3/T4, N+, G3, cancer located in the whole stomach, diffuse histological type according to Lauren.

2

Comparison of lymphatic system staging classifications in patients with gastric cance

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Spychała A. , Nowaczyk P. , Murawa D.

Polish Journal of Surgery

|

2015

|

tom 87(11)

551-557

EN

Despite an observed decrease in the incidence of gastric cancer, it still remains an important clinical problem. It is the fourth most common cancer in the world and the second cause of death in cancer patients. The quality of lymphadenectomy procedure and the number of analysed lymph nodes are both important factors influencing the treatment of the patient. The aim of the study was to compare the lymph node ratio staging system with the 6th and 7th edition of the TNM classification. Material and methods. A group consisting of 493 patients who underwent surgery in 1998–2010 due to gastric cancer was used to compare the staging systems. Following statistical analysis, the following cut-off points were adopted for the lymph node ratio for the purpose of comparison: 0, from 0.05 to 0.3, from 0.3 to 0.5 and over 0.5. Subsequently the homogeneity (using chi-square test for linear trend) and the predictive value of the different classifications (using Akaike information criterion) were assessed in order to compare the lymph node ratio staging system with the 6th and 7th edition of the TNM classification. Results. The lymph node ratio classification has a higher discriminatory value than the TNM classifications (higher linear trend result). What is more, the lymph node ratio classification (LNR) had a lower Akaike information criterion value, which means that it has a higher prognostic value than the other classifications. ROC curves and the area under the curve (AUC) were utilised for the analysis of predictive value of the different classifications in patients with gastric cancer. Conclusion. One may conclude, therefore, that the lymph node ratio staging system is the best classification of the lymphatic system in the presented group of patients.

3

Helicobacter pylori infection: from biology to clinical features

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Kolasińska K. , Kwiatkowski S. , Knap B. , Czerwiński G. , Poniewierka E. , Waszczuk E.

World Scientific News

|

2018

|

tom 107

171-184

EN

The discovery of bacterium Helicobacter pylori and the association of its occurrence with gastritis and peptic ulcer disease, was one of the most significant events of the 20th century. Almost 40 years have passed since that event, but we are still far from fully understanding of the impact of H. pylori infection on the human body. It is confirmed, that the infection causes chronic gastritis, which increases the risk of diseases such as: duodenal and/or gastric ulcer, gastric cancer and gastric MALT lymphoma. Nevertheless, constantly appear new studies linking H. pylori with many other disorders, often not directly related to the digestive system. Cardiovascular, neurological, hematological, dermatological, metabolic and also gynecological diseases are in sphere of assumptions. The authors of the following work attempted to select key information available in the medical literature on the discovery of H. pylori, epidemiology and pathophysiology of infection, as well as diseases to which it predisposes and the latest methods of eradication.

4

Can urea breath test (UBT) replace rapid urea test (RUT)?

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Nawacki Ł. , Czyż A. , Bryk P. , Kozieł D. , Stępień R. , Głuszek S.

Polish Journal of Surgery

|

2018

|

tom 90(5)

44-48

EN

Background: Helicobacter pylori (Hp) is classified by the International Agency for Research on Cancer (IARC) as a Group 1 carcinogen. Its influence on the carcinogenesis of gastric cancer has been confirmed in many research studies. The conclusion is obvious - early detection and eradication of Hp can prevent the development of the disease. Methods: The objective of the study was to analyze the clinical and practical value of Carbon-13 urea breath test (UBT) in patients hospitalized due to pain complaints in the upper abdomen and dyspeptic symptoms. Fifty patients were enrolled in the study. Each patient underwent urea breath test according to the instruction included by the producer. Thereafter, each patient included in the study group was subjected to endoscopy of the upper gastrointestinal tract with the biopsy of the mucosa to determine the urease activity with rapid urease test (RUT). Results: In the study group, 14 patients (28%) achieved a positive urease test result which was confirmed in RUT. Four (8%) patients, despite a positive breath test, did not have a positive result in urease activity test from gastric mucosa. In 2 cases (4%) despite negative result of UBT, urease activity was confirmed in gastroscopic sections. The remaining 30 patients (60%) had a negative result in both studies. Conclusions: The limited availability of the gold standard for diagnostics of upper gastrointestinal tract diseases (gastroscopy) is the basis for the search for new methods of detection of Helicobacter pylori infections. The urea breath test is a method of high sensitivity and specificity. The positive result of urea breath test may be the basis for inclusion of eradication therapy.

5

Laparoscopy-Assisted Total Gastrectomy with D2 Lymphadenectomy and Roux-En-Y Reconstruction - Case Report

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Papaj P. , Kozieł S. , Śleziński P. , Dobija-Kubica K. , Wróbel J.

Polish Journal of Surgery

|

2015

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tom 87

|

nr 1

43-46

EN

We report a case of patient with stage IIIb gastric cancer qualified for laparoscopy - assisted gastrectomy and our first impressions about this procedure. Total gastrectomy with complete omentectomy and extended lymphadenectomy (D2) was performed laparoscopically. The intestinal continuity was restored in a Roux-en-Y mode extracorporeally through the abdominal access system. The orogastric tube with anvil of the circular stapler was transorally introduced into the esophagus. Subsequently, intracorporeal stapling esophagojejunostomy was performed. There were no complications after the operation and the patient was discharged in good shape. Oncological radicality was sufficient and patient has undergone chemotherapy treatment.

6

Synchronous Gastric and Rectal Cancer in a 50 Year-Old Man – Case Report

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Pierko J. , Łukaszewicz J. , Sawicka-Pierko A. , Hady H. R. , Dadan J.

Polish Journal of Surgery

|

2012

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tom 84

|

nr 11

582-584

EN

The occurrence of synchronous tumors of the gastrointestinal tract is rarely observed in general surgery, diagnosis is frequently incidental, often intraoperatively. The aim of this study was to present a case of a 50 year-old male patient admitted to the Department of Gastroenterology, University Hospital in Białystok, due to abdominal pain, significant weakness and excretion of tarry stools. Imaging diagnostics revealed the presence of a large gastric tumor (histopathological type - adenocarcinoma), and single metastasis to the liver. The patient was qualified for total gastrectomy. Surgery was performed at the 1st Department of General and Endocrinological Surgery, University Hospital in Białystok. Rectal cancer, which was observed during the operation was removed simultaneously.

7

Endoscopic Ultrasonography (EUS) in Preoperative Staging of Gastric Cancer - Demand and Reality

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Meyer L. , Meyer F. , Schmidt U. , Gastinger I. , Lippert H. , Oraz Wschodnioniemiecka Grupa Na Rzecz Kontroli Jakości i Rozwoju Regionalnego w Chirurgii

Polish Journal of Surgery

|

2012

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tom 84

|

nr 3

152-157

EN

Exact pretherapeutic staging is considered to be essential for decision-making in the therapeutic algorithm of gastric cancer.The aim of the study was to characterize the role and value of EUS in the diagnostic and therapeutic management of gastric cancer in daily surgical practice.Material and methods. Thousand one hundred thirty nine patients with primary gastric cancer from 80 hospitals of each profile of care were enrolled in this systematic clinical prospective multicenter observational study over a time period of 12 months. The characteristics of the diagnostic management, in particular, of EUS were documented. The preoperative EUS findings were compared with the T stage (T1 to T4) and the N category (N+ or N-) revealed by the histopathologic investigation of the surgical specimen. By the mean of X2 test, the impact of EUS on the therapeutic decision-making was determined.Results. Pretherapeutic EUS was only performed in 27.4% (n=312) of all patients. Overall, the diagnostic accuracy for the T stage was 42.6% in average. The subgroup analysis showed the following results: T1, 31.5%; T2, 42.6%; T3, 65.2%; T4, 17.6%. The correct predictive value of the N category was 71.3% reaching a sensitivity of 69.7% and a specificity of 73.3%. Overstaging was observed in 45.8%, understaging in only 10.8%. Additional diagnostic information by EUS was only provided in 4.7% of subjects.Conclusions. The present study indicates the variability, limited reliability and only moderate acceptance of EUS in diagnosing gastric cancer in daily practice. In particular, the prediction of the T stage does not reach the data reported in the literature, which were mostly achieved in specific EUS studies.

8

Young Patient Age as the Cause of Delayed Diagnosis of Gastrointestinal Carcinoma

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Zaręba K. , Bandurski R. , Kędra B.

Polish Journal of Surgery

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2012

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tom 84

|

nr 6

317-321

EN

Most patients diagnosed with gastrointestinal carcinomas are older people. The above-mentioned fact may lead to an erroneous finding that the problem does not concern patients aged between 20 and 30 years. Unfortunately, this assumption is often the reason for late diagnosis and delayed treatment of these malignancies. The study presented an example of three patients subject to surgical management of gastrointestinal carcinomas at the II Department of General and Gastroenterological Surgery, Medical University in Białystok.

9

Hiatus hernia – Late complication after gastrectomy followed by double tract reconstruction (DTR): a case report

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Bandurski R. , Gryko M. , Dawidziuk T. , Zaręba K. , Cepowicz D. , Kędra B.

Progress in Health Sciences

|

2014

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tom 4

|

nr 1

265-268

XX

In patients with dyspeptic symptoms who have been treated for gastric cancer, other medical conditions apart from cancer recurrence should be considered. A long small-bowel loop left after esophagogastric anastomosis, flaccid diaphragmatic crura, and several external factors such as hard physical exertion can promote the development of postoperative hiatus hernia. The authors of this paper present a rare case of hiatus hernia considered a late complication of primary surgery performed due to gastric cancer. The 63-year-old patient had undergone total gastrectomy with double tract reconstruction (DTR) six years earlier. Gastrectomy was performed with extended lymphadenectomy (>D2). Histopathologic examination of tumor specimens showed mucinous adenocarcinoma with no lymph node metastasis and no distant metastases (pathologic staging: pT2, pN0, pM0). After six years, the patient was admitted to the 2nd Department of General and Gastroenterologic Surgery with abdominal pains, malaise, weight loss and feeling of fullness after small meals. Diagnostic procedures performed showed the presence of hiatus hernia, a very rare complication after this particular surgery, with no features of cancer recurrence. The patient was underwent surgery and the hernia was successfully repaired.

10

Pulmonary lymphangitic carcinomatosis in the course of gastric cancer - Case report

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Witczak A. , Prystupa A. , Zamecka M. O. , Bilan A. , Krupski W. , Mosiewicz J.

Journal of Pre-Clinical and Clinical Research

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2014

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tom 08

|

nr 2

EN

Pulmonary lymphangitic carcinomatosis is a special type of diffuse metastasis of carcinoma in the lymphatic vessels of the lungs. Lymphangitic carcinomatosis is commonly observed in malignancies of the breast, lung, pancreas, colon and cervix as a strong marker for poor prognosis. Presenting with common respiratory symptoms, it may be easily misdiagnosed as other pulmonary interstitial diseases. Pulmonary lymphangitic carcinomatosis is a rare manifestation of metastatic gastric cancer. The presented case describes a patient with pulmonary lymphangitic carcinomatosis in the course of stomach cancer. The primary diagnosis was established based on the exclusion of other interstitial lung diseases, with the use of imaging techniques as well as biochemical, microbiological and cytological findings. The patient’s general condition was very severe, rendering him bedridden and therefore not eligible for any treatment, including chemotherapy. He died suddenly before final verification of the diagnosis. Pulmonary lymphangitic carcinomatosis should be suspected in patients with advanced gastric cancer, presenting with symptoms and signs of respiratory disease. Imaging techniques are mostly helpful to establish the diagnosis.

11

Expression of EpCAM protein in gastric cancer cells may contribute to its histogenesis

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Nizioł M. , Kuczyńska P. , Misiura M. , Zińczuk J. , Zaręba K. , Ustymowicz W. , Ćwiklińska-Dworakowska M. , Baszun M. , Jelski S. , Guzińska-Ustymowicz K. , Pryczynicz A.

Progress in Health Sciences

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2018

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tom 8(1)

74-79

EN

Introduction: EpCAM protein belongs to adhesion molecules of epithelial cells. It mediates in the homophile adhesion cell-cell reactions. EpCAM protein expression can be observed in the majority of healthy normal cells. However, mutations in EpCAM gene may lead to an increased risk of cancer development. The aim of the study was to assess EpCAM protein expression in the correlation with chosen clinical and histological parameters in gastric cancer. Materials and Methods: EpCAM protein expression was evaluated immunohistochemically in 88 patients diagnosed with gastric cancer. Results: An increase in EpCAM protein expression was demonstrated in cancer cells compared to normal gastric mucosa (59.3% cancers with the positive expression of EpCAM protein). The increased EpCAM protein expression was observed in patients with a histological type of adenocarcinoma without a mucinous component than in those with adenocarcinoma with a mucinous component (p=0.028). The higher expression of this protein was observed also in the intestinal type according to the Lauren classification (p=0.037). The expression of the protein was lower in the diffuse type of cancer. Additionally, an increase in EpCAM protein expression was revealed in cancers infiltrating to the blood vessels (p=0.013). Conclusions: A correlation between EpCAM expression and adenocarcinoma without a mucinous component as well as the intestinal type according to the Lauren classification may prove a role of this protein in the histogenesis of gastric cancer. Moreover, its positive expression is related to cancerous cells infiltrating to the blood vessels, which may suggest a role of EpCAM protein in the early stages of gastric cancer metastases.

12

Evaluation of Endostatin and EGF Serum Levels in Patients with Gastric Cancer

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Masiak W. , Szponar A. , Chodorowska G. , Dąbrowski A. , Pedowski T. , Wallner G.

Polish Journal of Surgery

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2011

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tom 83

|

nr 1

42-47

EN

The aim of the study was to assess angiogenesis markers - endostatin and endothelial growth factor (EGF) as markers of detection of gastric carcinoma.Material and methods. The study involved 20 patients with colorectal cancer (10 women, 10 men) aged 35 - 75 years, mean age = 55 years ± 11.2 who referred to the 2nd Department of General Surgery, Medical University in Lublin between June 2008 and June 2009. The control group comprised 10 volunteers (6 women, 4 men) who underwent upper gastrointestinal (GI) endoscopy due to the reflux disease and in whom gastric cancer was not diagnosed.Results. The mean endostatin concentration in controls was 5.21 ng/mL ± 1.37. Mean concentrations in patients with gastric cancer were higher than those in controls - 5.91 ng/mL ± 1.5. The difference was not statistically significant (p= 0.714). The EGF concentration in the control group was 28.19 pg/mL ± 12.94. EGF concentrations in patients with gastric cancer were higher compared to the control group - 28.8 pg/mL ± 12.63. The difference was not statistically significant (p= 0.85). The mean concentration of endostatin before the operation was 5.91 ng/mL ± 1.5 and after surgery was 5.33 ng/mL ± 2.01, the difference was not statistically significant.Conclusions. Blood endostatin and EGF quantitative determinations probably is not useful for detection of gastric carcinoma and effectiveness of treatment.

13

Double Tract Reconstruction (DTR) - An Alternative Type of Digestive Tract Reconstructive Procedure After Total Gastrectomy - Own Experience

80%

Bandurski R. , Gryko M. , Kamocki Z. , Okulczyk B. , Zaręba K. , Piotrowski Z. , Kędra B.

Polish Journal of Surgery

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2011

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tom 83

|

nr 2

70-75

EN

The only proven, effective therapy in case of the gastric cancers is surgery.The aim of the study. The most common procedure which is made in such a situation is total resection of the stomach. In our publication we would like to present and to recommend a very rare made type of the reconstructive procedures after total gastrectomy, which is called "double tract reconstruction" (DTR). This type of reconstruction is occasionally made mainly in Japan.Material and methods. Double tract reconstruction has been made in 2nd Department of General and Gastroenterological Surgery since 2000. Till today 75 patients were treated with this method.Results. The frequency of complications after double tract reconstruction was occasional, and there were no differences between this procedure and Roux-en-Y method of the reconstruction. There were no differences in the time of the operation between this two methods. The most important advantage of this method is that duodenal passage is extant. Because of that the endoscopic examination of papilla Vateri can be made.Conclusions. We would like to recommend this method as an alternative to Roux-en-Y procedure because of its simplicity and safeness.

14

Brain metastasis as the first symptom of gastric cancer – case report and literature review

80%

Murawa D. , Nowaczyk P. , Szymkowiak M. , Karaszewska B.

Polish Journal of Surgery

|

2013

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tom 85

|

nr 7

401-406

EN

The study presented a patient with asymptomatic gastric cancer, in whom the first symptom was metastasis to the brain. The patient was initially diagnosed by a neurologist and subject to surgical intervention in the area of residence, where he underwent craniotomy with the excision of the metastatic lesions located in the occipital lobe. The histopathological examination revealed the presence of adenocarcinoma metastases. Following complex diagnostics the patient was diagnosed with cardial carcinoma, being subject to cerebral radiotherapy and chemotherapy. The patient was then referred to surgery at the Wielkopolska Cancer Center in Poznań. After final exclusion of disease dissemination (by means of PET-CT) the patient underwent total gastrectomy with D2 lymphadenectomy, and gastrointestinal tract reconstruction by means of the Roux-en-Y method. The histopathological examination result was as follows: tubular-papillary G2 adenocarcinoma (intestinal type), pT2 pN0 (23 evaluated lymph nodes without cancer metastasis), vascular neoplastic emboli, and positive HER2 protein expression. After surgery the patient was subject to adjuvant chemotherapy. Control brain CT examinations revealed the presence of 4 recurrent metastatic lesions-the patient was disqualified from stereotactic radiation therapy and was subject to palliative chemotherapy. The discussion presented the problem of treating patients with stage IV gastric cancer, including current management guidelines, as well as literature review concerning the treatment of patients with diagnosed gastric cancer and brain metastases.

15

Advisability of Palliative Resections in Incurable Advanced Gastric Cancer

80%

Al-Amawi T. , Świder-Al-Amawi M. , Halczak M. , Wojtasik P. , Kładny J.

Polish Journal of Surgery

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2011

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tom 83

|

nr 8

449-456

EN

Effective treatment is the primary objective of surgeon in the treatment of advanced gastric cancer. Poor prognosis and significant advancement of gastric cancer at the time of diagnosis are decisive factors for the only possible surgical management method being palliative procedures.The aim of the study was the evaluation of the value of palliative resection procedures in patients with advanced gastric cancer.Material and methods. The subject in the study was a group of 105 patients with gastric adenocarcinoma at stage 4 of advancement, in whom curative treatment was not possible. The group constituted 44.5% of patients operated on due to gastric cancer at the Department of General and Oncological Surgery, PUM, in the years 1998-2009. The patients were divided into two groups: the first one comprised 44 patients post palliative resections, the second - 61 patients post non-resection procedures. The subject of analysis were early and late treatment results post palliative resections, and they were compared with the treatment results post non-resection procedures.Results. Palliative resections were performed in 44 patients (19 females and 25 males), while in 61 patients (38 males and 23 females) non-resection procedures were performed. Postoperative complications were observed in 25% of patients in the group post palliative resections and in 11.5% in the group of patients without the resection of primary focus. In-hospital mortality stood at 4.5% in the group post palliative resections and 4.8% in the group post non-resection procedures. The percentage of 1-year and 4-year survival post palliative resections stood at 43% and 8.8%, respectively. In the group without the resection of primary focus, 16% survived 1 year and nobody survived 2 years. Conclusions. Palliative resections improve the survival of patients with incurable gastric cancer and should be considered if only the loco-regional conditions are favourable.

16

The Influence of Splenectomy Performed Simultaneously with Gastrectomy on Postoperative Complications in Patients with Gastric Cancer Undergoing Surgery with the Intention to Treat

70%

Głuszek S. , Kot M. , Kuchinka J. , Matykiewicz J.

Polish Journal of Surgery

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2015

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tom 86

|

nr 7

312-318

EN

Despite the growing understanding of the pathophysiological processes in the perioperative period and significant advancements in surgical techniques, operative treatment for gastric cancer remains a challenge for surgeons, especially because the primary procedure of total or nearly total gastrectomy must at times be extended by the resection of other organs. The aim of the study was to asses the influence of concomitant splenectomy in patients undergoing curative surgery for gastric cancer on postoperative complications. Material and methods. The study population consisted of 258 patients who underwent surgical treatment for gastric cancer with the intention to treat. The study assessed the influence of extending the surgical intervention by splenectomy on postoperative complications, both general and surgical, including the most severe of these, i.e. oesophago-gastric anastomotic leakage, duodenal stump leakage and peritoneal fluid infections. Results. Among the 258 gastric cancer patients receiving curative surgical treatment, the most common simultaneous intervention was splenectomy: 42/258 (16.3%), which was also accompanied by partial pancreatectomy in 8 cases. The number of surgical postoperative complications, major and minor, was similar in both subgroups: with and without splenectomy. Minor general complications, such as pyrexia with no clinically apparent reason, atelectasis, pneumonia and pleural effusion were statistically significantly more common in the subgroup with splenectomy (p=0.0001). Conclusion. Splenectomy performed concomitantly with gastrectomy for gastric cancer increases the risk of minor general complications. However, it does not increase the risk of severe surgical complications, such as oesophago-intestinal anastomotic leakage and does not increase the risk of death

17

Comparison of expression of selected proteins in the cells of intestinal and diffuse type gastric cancer – immunohistochemical analysis

70%

Gryko M. , Prycznicz A. , Dawidziuk T. , Zińczuk J. , Cepowicz D. , Kamocki Z. , Kedra B. , Guzińska-Ustymowicz K.

Progress in Health Sciences

|

2014

|

tom 4

|

nr 1

158-164

XX

Introduction: Despite the progress in gaining knowledge about carcinogenesis, it is still unclear what processes are directly responsible for the differentiation of gastric cancer into its intestinal or diffuse form. Dividing of these two forms is based on one of the oldest, yet still commonly used classifications – the classification of Lauren. There are many factors that may influence the formation of gastric tumors of various aggressiveness. Purpose: To evaluate the expression of proteins: fragile histidine triad (FHIT), E-cadherin, α-catenin, γ-catenin, cathepsin B, epidermal growth factor (EGF), HER-2, MMP-9, MCM-2, Bak, Bax, BID, Bcl-XL, p53, FasL, Bcl-2, caspase-8, procaspase-3 in gastric cancer cells, depending on the type of tumor by Lauren classification. Materials and methods: Study group consisted of 91 patients treated surgically for gastric cancer in the Second Department of General and Gastroenterological Surgery, Medical University of Bialystok in years between 2000 and 2006. Results: It is shown, that the expression of E-cadherin was significantly higher in the Lauren I gastric cancer cells than in Lauren II. In case of caspase-8 there has been significantly less frequent expression of this protein in Lauren I gastric cancer cells compared to Lauren II. The authors describe no statistically significant differences in the expression of other proteins taken into consideration. Conclusions: These results suggest the role of adhesion and apoptosis-related proteins in the development of two different types of gastric cancer according to Lauren’s classification.

18

Hypoxic regulation of PFKFB-3 and PFKFB-4 gene expression in gastric and pancreatic cancer cell lines and expression of PFKFB genes in gastric cancers

60%

Bobarykina A. Y. , Minchenko D. O. , Opentanova I. L. , Moenner M. , Caro J. , Esumi H. , Minchenko O. H.

Acta Biochimica Polonica

|

2006

|

tom 53

|

nr 4

789-799

EN

Previously we have shown that hypoxia strongly induces the expression of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 and -4 (PFKFB-3 and PFKFB-4) genes in several cancer cell lines via a HIF-dependent mechanism. In this paper we studied the expression and hypoxic regulation of PFKFB-4 and PFKFB-3 mRNA as well as its correlation with HIF-1α, HIF-2α, VEGF and Glut1 mRNA expression in the pancreatic cancer cell line Panc1 and two gastric cancer cell lines MKN45 and NUGC3. This study clearly demonstrated that PFKFB-3 and PFKFB-4 mRNA are expresses in MKN45, NUGC3 and Panc1 cancers cells and that both genes are responsive to hypoxia in vitro. However, their basal level of expression and hypoxia responsiveness vary in the different cells studied. Particularly, PFKFB-3 mRNA is highly expressed in MKN45 and NUGC3 cancer cells, with the highest response to hypoxia in the NUGC3 cell line. The PFKFB-4 mRNA has a variable low basal level of expression in both gastric and pancreatic cancer cell lines. However, the highest hypoxia response of PFKFB-4 mRNA is found in the pancreatic cancer cell line Panc1. The basal level of PFKFB-4 protein expression is the highest in NUGC3 gastric cancer cell line and lowest in Panc1 cells, with the highest response to hypoxia in the pancreatic cancer cell line. Further studies showed that PFKFB-3 and PFKFB-4 gene expression was highly responsive to the hypoxia mimic dimethyloxalylglycine, a specific inhibitor of HIF-α hydroxylase enzymes, suggesting that the hypoxia responsiveness of PFKFB-3 and PFKFB-4 genes in these cell lines is regulated by the HIF transcription complex. The expression of VEGF and Glut1, which are known HIF-dependent genes, is also strongly induced under hypoxic conditions in gastric and pancreatic cancer cell lines. The levels of HIF-1α protein are increased in both gastric and pancreatic cancer cell lines under hypoxic conditions. However, the basal level of HIF-1α as well as HIF-2α mRNA expression and their hypoxia responsiveness are different in the MKN45 and NUGC3 cancer cells. Thus, the expression of HIF-1α mRNA is decreased in both gastric cancer cell lines treated by hypoxia or dimethyloxalylglycine, but HIF-2α mRNA expression is not changed significantly in NUGC3 and slightly increased in MKN45 cells. Expression of PFKFB-4 and PFKFB-3 was also studied in gastric cancers and corresponding nonmalignant tissue counterparts from the same patients on both the mRNA and protein levels. The expression of PFKFB-3 and PFKFB-4 mRNA as well as PFKFB-1 and PFKFB-2 mRNA was observed in normal human gastric tissue and was increased in malignant gastric tumors. The basal level of PFKFB-4 protein expression in gastric cancers was much higher as compared to the PFKFB-3 isoenzyme. In conclusion, this study provides evidence that PFKFB-4 and PFKFB-3 genes are also expressed in gastric and pancreatic cancer cells, they strongly respond to hypoxia via a HIF-1α dependent mechanism and, together with the expression of PFKFB-1 and PFKFB-2 genes, possibly have a significant role in the Warburg effect which is found in malignant cells.

19

12-O-tetradecanoylphorbol-1,3-acetate induces the negative regulation of protein kinase B by protein kinase Calpha during gastric cancer cell apoptosis

60%

Zhang B. , Xia C.

Cellular and Molecular Biology Letters

|

2010

|

tom 15

|

nr 3

377-394

EN

The PKB signaling pathway is essential for cell survival and the inhibition of apoptosis, but its functional mechanisms have not been fully explored. Previously, we reported that TPA effectively inhibited PKB activity and caused PKB degradation, which was correlated with the repression of PKB phosphorylation at Ser473. In this study, we focus on how PKB is regulated by TPA in gastric cancer cells. One of the TPA targets, PKCα, was found to mediate the inhibition of PKB phosphorylation and degredation caused by TPA. Furthermore, TPA induced the import of PKCα into the nucleus, where PKCα exerted an inhibitory effect on PKB expression and phosphorylation. As a result, cancer cell proliferation was arrested. Our study characterizes a novel function of PKCα in mediating the negative regulation of PKB by TPA, and suggests a potential application in the clinical treatment of gastric cancer.

20

Wybrane aspekty opieki pielęgniarskiej realizowanej wobec pacjenta w przebiegu raku żołądka

60%

Kot N. , Żelazek P.

Innowacje w Pielęgniarstwie i Naukach o Zdrowiu

|

2019

|

tom 4

|

nr 3

92-100

EN

Introduction. Gastric cancer is malignant cancer, which is generated in the internal layers of the stomach. This type of cancer is often asymptomatic. There is a higher incidence of gastric cancer in patients over 50 years of age , especially in men. Aim. The aim of this study was to analyse selected aspects of nursing care in patients with gastric cancer. Material and methods. Analysis of available literature related to clinical aspects and nursing care in patients with gastric cancer and the activities of mapping medical diagnosis together with nursing care with the use of ICNP® dictionary. Results. The use of ICNP® classification allows to reflect patient's health situation in patients with gastric cancer and to plan medical care based on uniform professional terminology.

PL

Wstęp. Rak żołądka jest nowotworem złośliwym, który wywodzi się z komórek warstwy wewnętrznej narządu. Cechuje się długim okresem bezobjawowym. Zapadalność na raka żołądka zwiększa się po 50 roku życia, szczególnie w populacji mężczyzn. Cel. Celem pracy była analiza wybranych aspektów opieki pielęgniarskiej realizowanej wobec pacjenta w przebiegu raka żołądka. Materiały i metody. W pracy zastosowano metodę analizy piśmiennictwa poświęconego zagadnieniom klinicznym i opiece nad chorym z rakiem żołądka oraz mapowanie diagnoz i interwencji pielęgniarskich przy użyciu słownika ICNP®. Wyniki i wnioski. Zastosowanie klasyfikacji ICNP® umożliwia odzwierciedlenie sytuacji zdrowotnej pacjenta w przebiegu raka żołądka oraz planowanie założeń opieki pielęgniarskiej w oparciu o ujednoliconą terminologię zawodową.