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EN
The paper describes the epizootic situation of rabies in the Warmińsko-Mazurskie voivodeship between 2001-2005. The vaccination of wild animals has been conducted in the voivodeship since May 2001. In the year 2001, 387 cases of rabies, including 93 (20%) in domestic animals and 294 (80%) in wild animals, were recorded respectively. Among domestic animals the highest incidence of the disease was found in cattle - 53 cases (14%), in cats - 24 cases (6%) and in dogs - 16 cases (4%). Among wild animals, rabies was found in 184 foxes (47%), 85 racoon dogs (22%), 8 martens (2%), 7 badgers (2%), and 6 polecats (2%). In the year 2005, 78 cases of rabies, including 29 (37%) in domestic animals and 49 (63%) in wild animals, were recorded respectively. Regarding domestic animals, the incidence of the disease was found in cattle - 23 cases (29%), cats - 3 cases (4%) and dogs - 2 cases (3%). Among wild animals, rabies was found in 39 foxes (50%), 8 raccoon dogs (10%) and 2 bats (3%). This suggests that after 5 years of oral vaccination the main reservoir of the rabies virus in the Warmińsko-Mazurskie voivodeship is still in wild animals, primarily foxes. Observations to date indicate that there remains a necessity to continue the wild animal immunization campaign.
EN
In recent years a considerable improvement has been reported in the field of molecular biology. New molecular methods have replaced classic diagnostic techniques. Apart from pathogen detection, they also allow pathogen quantification in a relatively short time. For instance, real time PCR, thanks to using DNA-binding fluorophores or labelled oligonucleotide probes, allows the detection of virus and monitoring of each cycle of reaction. The use of complementary probes makes this method sensitive and specific. Real time PCR is widely applied, especially in microbiological and virological laboratories, also for rabies diagnostics. Additionally, it is an excellent tool in biomedical and molecular research. For example, it is used for relative and absolute quantification of gene expression and the identification of mutations that might be a key issue in some diseases. Recently, real time PCR seems to become a standard technique in many diagnostic laboratories.
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