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EN
Characteristics of muscle fibers of breast and leg muscles of grey partridges Perdix perdix. The experimental material included grey partridges Perdix perdix L. planned for reintroduction into the natural habitat, reared at the Game Breeding Center on the area of Mazovia Province. 10 females and 10 males were selected for slaughter. The birds were slaughtered in poultry abattoir and specimens of their pectoral superficial muscle (pectoralis superfi cialis) and biceps femoris muscle (biceps femoris) were sampled. A greater diameter of fibers was determined for breast muscles. The study did not show any effect of sex on muscle fiber diameter of breast and leg muscles of grey partridges Perdix perdix.
PL
Charakterystyka włókien mięśniowych mięśni piersiowych i nóg kuropatwy polnej Perdix perdix. Materiał badawczy stanowiły kuropatwy polne Perdix perdix L. przeznaczone do wsiedlenia do środowiska naturalnego, odchowywane w Ośrodku Hodowli Zwierzyny na terenie województwa mazowieckiego. Do uboju wybrano po 10 kur i 10 kogutów. Ptaki ubito, a następnie pobrano próbki mięśni piersiowych (pectoralis superfi cialis) oraz mięśni nóg (biceps femoris). Większą średnicą włókien charakteryzowały się mięśnie piersiowe. Nie wykazano wpływu płci na średnicę włókien mięśni piersiowych i mięśni nóg kuropatwy polnej Perdix perdix.
EN
It has been reported that various types of mammalian muscle fibers differ regarding the content of several metabolites at rest. However, to our knowledge no data have been reported in the literature, concerning the muscle energetic status at rest in high class athletes when considering the dominant and non-dominant leg separately. We have hypothesised that due to higher mechanical loads on the dominant leg in athletes, the metabolic profile in the dominant leg at rest in the calf muscles, characterized by [PCr], [ADPfree], [AMPfree] and GATP, will significantly differ among endurance athletes, sprinters and untrained individuals. In this study we determined the GATP and adenine phosphates concentrations in the dominant and non-dominant legs in untrained subjects (n = 6), sprinters (n = 10) and endurance athletes (n = 7) at rest. The (mean ± SD) age of the subjects was 23.4 ± 4.3 years. Muscle metabolites were measured in the calf muscles at rest, by means of 31P-MRS, using a 4.7 T superconducting magnet (Bruker). When taking into account mean values in the left and right leg, phosphocreatine concentration ([PCr]) and GATP were significantly lower (p<0.05, Wilcoxon-Mann-Whitney test), and [ADPfree] was significantly higher (p = 0.04) in endurance athletes than in untrained subjects. When considering the differences between the left and right leg, [PCr] in the dominant leg was significantly lower in endurance athletes than in sprinters (p = 0.01) and untrained subjects (p = 0.02) (25.91 ± 2.87 mM; 30.02 ± 3.12 mM and 30.71 ± 2.88 mM, respectively). The [ADPfree] was significantly higher (p = 0.02) in endurance athletes than in sprinters and untrained subjects (p = 0.02) (42.19 ± 13.44 µM; 27.86 ± 10.19 µM; 25.35 ± 10.97 µM, respectively). The GATP in the dominant leg was significantly lower (p = 0.02) in endurance athletes than in sprinters and untrained subjects (p = 0.01) (-60.53 ± 2.03 kJ·M-1; -61.82 ± 1.05 kJ·M-1, -62.29 ± 0.73 kJ·M-1, respectively). No significant differences were found when comparing [PCr], [ADPfree], [AMPfree], [Mg2+free], GATP in the dominant leg and the mean values for both legs in sprinters and untrained subjects. Moreover, no significant differences were found when comparing the metabolites in non-dominant legs in all groups of subjects. We postulate that higher [ADPfree] and lowerGATP at rest is a feature of endurance-trained muscle. Moreover, when studying the metabolic profile of the locomotor muscles in athletes one has to consider the metabolic differences between the dominant and non-dominant leg.
EN
Single, functionally isolated motor units were studied in the medial gastrocnemius (MG) muscle of cats and rats. Axons of their motoneurons were stimulated with trains of pulses at frequencies increasing from 1 to 150 Hz and forces developed by muscle fibers were measured and force-frequency curves were compared between species. The following observations were made: (1) the most steep parts of curves (related to unfused tetani of motor units) begun at lower frequencies of stimulations in all types of feline motor units, (2) for fast motor units, the same relative values of force of unfused tetani were achieved at significantly lower frequencies of stimulations in the cat than in the rat. Twitch time parameters of both species influenced the course of force-frequency curves. It was showed that the contraction times of feline units varied in the wide range (21-81 ms), and these units reached 60% of the maximum force at stimulation frequencies between 10 and 38 Hz. On the other hand, contraction times of rat units ranged from 10 to 34 ms, whereas stimulation frequencies necessary to reach 60% of the maximum force varied considerably, from 12 to 65 Hz. The correlations between the above parameters were found for motor units of each species. However, the regression lines drown for the collected population of cat and rat units did not form linear continuity. Thus it seems that interspecies differences in the twitch contraction times do not fully explain different force-frequency relationships in mammalian skeletal muscles.
EN
The experiment was conducted with 60 slow-growing Hubbard JA 957 broilers and with 60 Silkies chickens reared until the 63rd day of life, in three replications, 20 birds each, in a closed building on litter. A three-stage feeding program was applied in the rearing period. Feed mixtures used in the experiment did not differ in contents of nutrients. In the first stage of rearing (till 21 days of life) the chickens were fed a standard starter feed mixture containing: 20.98% of total protein, 2,845 kcal/kg of me-tabolizable energy, 1.17% of lysine and 0.98% of met. + cys. In the period from 22 to 56 days of life they were receiving a grower type mixture containing: 20.0% of total protein, 2,900 kcal/kg of energy, 1.04% of lysine and 0.88% of met. + + cys., whereas contents of these components in the finisher type mixture administered till the end of the rearing period were as follows: 18% of protein, 2,965 kcal/kg of EM, 0.90% of lysine and 0.78% of met. + cys. On day 63 of rearing, 12 cockerels and 12 hens were selected random from each group. The birds were slaughtered in a poultry slaughter house, and specimens of their major breast muscle (m. pectoralis superficialis) and thigh muscle (biceps femoris) were sampled for analyses. The breast and leg muscles of the birds were characterized by diversified diameters of muscle fibers depending on bird genotype. The greatest diameters were found in breast and leg muscles of the slow-growing Hubbard JA 957 broilers, which was confirmed statistically (P < 0.01). It was also demonstrated that the mean diameter of muscle fibers of Silkies chickens was more than two times smaller compared to the Hubbard JA 957 chickens.
PL
Profil histologiczny mięśni piersiowych i nóg kurcząt Jedwabistych oraz wolno rosnących Hubbard JA 957. Badanie przeprowa- dzono na 60 brojlerach wolno rosnących Hubbard JA 957 oraz 60 kurczętach Jedwabistych odchowywanych do 63. dnia życia, w trzech powtórzeniach po 20 szt., w zamkniętym budynku na ściółce. W okresie odchowu zastosowano trzyfazowy program żywienia. Mieszanki użyte w doświadczeniu nie różniły się pod względem zawartości składników pokarmowych. Kurczęta w pierwszym okresie odchowu do 21. dnia życia żywiono standardową mieszanką typu starter: 0 zawartości 20,98% białka ogólnego, 2845 kcal na kg energii metabolicznej, 1,17% lizyny i 0,98% met. + cys. W okresie od 22. do 56. dnia stosowano mieszankę typu grower, zawierającą 20,0% białka ogólnego, 2900 kcal na kg energii metabolicznej (EM), 1,04% lizyny i 0,88% met. + cys. Zawartość wcześniej wymienionych składników w mieszance fmiszer, stosowanej do końca odchowu, wynosiła odpowiednio: 18% białka, 2965 kcal na kg EM, 0,90% lizyny i 0,78% met. + cys. W 63. dniu odchowu wybrano z każdej grupy po 12 kogutów i 12 kur o masie ciała zbliżonej do średniej w grupie. Kurczęta ubito w ubojni drobiu i pobrano wycinki mięśnia piersiowego powierzchniowego (m. pectoralis supetficialis) oraz dwugłowego uda (biceps femoris). Mięśnie piersiowe i nóg ptaków doświadczalnych charakteryzowała zróżnicowana średnica włókien mięśniowych w zależności od genotypu. Największą średnicą charakteryzowały się mięśnie piersiowe 1 nóg ptaków wolno rosnących Hubbard JA 957, co zostało potwierdzone statystycznie (P < 0,01). Stwierdzono, że średnica włókien mięśniowych kurcząt Jedwabistych była o ponad połowę mniejsza w porównaniu do kurcząt Hubbard JA 957.
EN
The morphohistochemical profile of red and pink muscles from the caudal portion of the trunk at the height of the horizontal septum in the Carassius auratus gibelio and Perca fluviatilis 20-25 cm long from the Gare.nica (Croatia) fish farm have been studied. Muscle samples, taken on arrival from live fish, were frozen in liquid nitrogen, cut on the cryo cut into 10 µm thick slices stained with hemalum and eosin and with the procedure for the detection of succinate dehydrogenase (SDH). The diameter of muscle fibers from the obtained preparations was measured using a micrometer scale with 10 µm divisions on a Nikon microscope. The red muscle of the Carassius auratus gibelio and Perca fluviatilis is known to have a triangular cut. It is positioned laterally beneath the skin and runs craniocaudally along the horizontal septum. Underneath the surface red muscle there is a pink muscle, shorter than the red one. In the Carassius auratus gibelio it is wide and divided into the surface (pink I) and deep (pink II) layer, while in Perca fluviatilis it is narrow, short and unchanged, but comprises a narrow transitional zone towards the red muscle. The red muscle is composed of red muscle fibers of small diameter with potent SDH activity. The pink muscle is composed of two types of fibers . intermediary small-diameter muscles of pronounced SDH activity and large-diameter muscles of less pronounced SDH activity as well as white muscles of large diameter with poor SDH activity. Intermediary fibers in the Carassius auratus gibelio accounted for 77.80% of the fibers in the pink I layer and 44.90% of the fibers in the pink II layer. White fibers in the same fish accounted for 22.20% of the fibers in the pink I layer and 55.10% of the fibers in the pink II layer. Intermediary fibers in the pink muscle of the Perca fluviatilis accounted for 58.80% fibers, whereas white fibers accounted for 41.20% of all fibers.
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