Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na https://bibliotekanauki.pl
Ograniczanie wyników
Czasopisma help
Lata help
Autorzy help
Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników

Znaleziono wyników: 318

Liczba wyników na stronie
first rewind previous Strona / 16 next fast forward last
Wyniki wyszukiwania
Wyszukiwano:
w słowach kluczowych:  test ELISA
help Sortuj według:

help Ogranicz wyniki do:
first rewind previous Strona / 16 next fast forward last
EN
The objective of the studies was development and use of a customized ELISA kit to diagnose mycoplasmal infections in poultry. The frozen strain of S-6 M. gallisepticum, concentrated and ultra-sounds processed, served as the antigen. The best extinction (OD) was noted with the antigen concentration of 100 µg/ml, sera diluted at 1:100 and conjugate diluted at 1:250. It was best to preserve antigen coated plates overnight at 4°C. The ELISA was used to detect specific anti-M. gallisepticum antibodies both in chickens experimentally infected and in hens and turkeys naturally infected with this microorganism. Basing on the calculated value S/P it was possible to compare the sensitivity of the ELISA with an agglutination test (SPA) and a hemagglutination inhibition test (HI). The value of S/P for positive sera was more than 0.5, while for negative sera, below 0.5. The value of S/P in the ELISA for sera of chickens experimentally infected with high positive titres in HI (320 and 640) was more than 2.5, and for sera of HI titre 80 these values of S/P ranged from 1.0 to 1.5. The investigation of naturally infected birds showed that S/P in the ELISA test for sera of full agglutinating properties (3+) was more than 2.0, and for individual sera it was up to 3.5. However, weakly positive sera in the SPAS have a negative or slightly positive S/P value (from 0.5 to 1.0). The ELISA test used to detect antibodies specific to M. gallisepticum proved to be more sensitive than the SPA and HI test.
EN
The aim of the study was to assess the seroprevalence of Equine rhinitis B viruses (ERBV) in the horse population of the south-eastern part of Poland. Selected horse farms, including breeding farms, stallion herds, purchasing centers and riding clubs were included in the studies. Blood samples were taken from 650 adult horses and foals of different age groups from 23 farms. Commercial ELISA test (Equivir IgG ELISA, Tridelta Development Limited, Ireland) has been used in the studies. On average specific antibodies to ERBV were found in 70.5% of the animals examined. The percentage of positive results of the serological survey was diverse in particular horse farms and ranged from 37.5% to 100% of the animals. It was demonstrated that the farm type and sex of horses did not influence the serological results, while the number of horses in the farm significantly influenced the serological results (P < 0.05).
EN
The aim of the study was to perform a preliminary evaluation of the ELISA test for the diagnosis of bovine tuberculosis in Polish conditions. The Idexx kit of immunoassays for detecting the presence of antibodies specific for Mycobacterium bovis in samples of cattle’s blood serum and plasma was used to examine a total of 50 serum samples from clinically healthy animals, 44 sera from cows with previous positive result of tuberculin tests, but negative in microbiological examination, 25 sera from animals from whose tissues strains of Mycobacterium bovis had been isolated (including 10 sera from cows with characteristic tuberculous lesions and 15 sera from animals without these lesions), and 16 serum samples from animals reacting positively for Johne’s disease. On the basis of the results of these examinations, the specificity of the test was determined at 100% and sensitivity at 90%. There were no cross-reactions, and the results indicate that the ELISA Idexx kit may be used as an important diagnostic tool in the diagnosis of bovine tuberculosis.
PL
U osób z neuroinfekcją enterowirusową analizowano poziom przeciwciał w surowicy i płynie mózgowo-rdzeniowym. Porównano następujące odczyny: wiązania dopełniacza, neutralizacji, ELISA dla enterowirusowych IgG i IgM. Badanie testem ELISA IgM dla enterowirusów w próbce surowicy z pierwszego pobrania pozwala na potwierdzenie zakażenia u 75% badanych osób, a ocena miejscowej produkcji IgG w oun w tym samym czasie u około 70% badanych, co wskazuje na przydatność tej metody do szybkiej diagnostyki zakażeń enterowirusami.
EN
The usefulness of the methods was compared: complement fixation test (CFT), neutralization test (NT) and ELISA IgG and IgM against enteroviruses for the evaluation of specific immune reaction in sera and cerebrospinal fluid (CSF) samples of patients with confirmed enterovirus infections. The criteria were established for the assessment of ELISA results in rapid diagnosis of enterovirus neuroinfections. The criteria accepted by the producer lowered the sensitivity of the method and the possibility of recognition of local synthesis of antibodies in the CNS. The use of serum negative in CFT and negative CSF as reference for the determination made possible using of that kit for rapid diagnosis of neuroinfections. The modified ELISA IgG test makes possible determination of antibodies in CSF and serum, and accepting the generally recognized criteria for local production of antibodies in the CNS the ELISA test makes possible rapid diagnosis of neuroinfections which is not possible by other methods.
PL
Gluten to jeden z najpowszechniej występujących alergenów roślinnych, powodujący u osób wrażliwych wystąpienie celiakii (glutenozależnej choroby trzewnej). Osoby cierpiące na tę chorobę muszą dla zachowania zdrowia pozostawać przez całe życie na diecie bezglutenowej. Z tego względu istotne znaczenie ma badanie zawartości glutenu (gliadyny) w żywności, zarówno specjalnego przeznaczenia żywieniowego, jak i w wybranych produktach ogólnego spożycia. Z uwagi na wagę zagadnienia, w Zakładzie Wartości Odżywczych Żywności Instytutu Żywności i Żywienia wdrożono zatwierdzoną przez Kodeks Żywnościowy, immunoenzymatyczną metodę (ELISA Mendez R5) oznaczania gliadyny w żywności przy użyciu gotowych zestawów testowych.
EN
Gluten is one of the most common plant allergens which causes celiac disease in sensitive individuals. Celiac persons must stay gluten free diet for life to maintain health. Thus it is very important to analyse gluten (gliadin) content in food - both particular nutritional uses and also food for normal consumption. Taking this into consideration ELISA Mendez R5 method of gluten determination temporarily endorsed by Codex Alimentarius FAO/WHO was implemented in department of Nutritional Value of Food at National Food and Nutrition Institute.
EN
The aim of the study was to develop a sensitive and specific enzyme-linked immunosorbent assay (ELISA) for determining K88 fimbriae in E. coli strains. The presented test is based on the isolated and purified K88ab and K88ac fimbriae as the solid-phase antigens and specific anti-K88ab and anti-K88ac polyclonal antibodies, respectively. The ELISA described was shown to be highly specific and sensitive. It was possible to detect K88 |imbriae in a bacterial concentration of 5 x 10' - 3 x l(r cells per ml. Our test also provides a convenient method for determining the K88ab and K88ac antigenic variants of K88 fimbriae. By means of the described procedure it was shown that the K88ac variant predominates in E. coli strains isolated from the enteric form of colibacillosis. The ELISA can also be used for the selection of the K88-positive E. coli strains for vaccine production purposes.
first rewind previous Strona / 16 next fast forward last
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.