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  1. 6 Folia Histochemica et Cytobiologica
  2. 5 Diagnostyka Laboratoryjna
  3. 4 Acta Biochimica Polonica
  4. 3 Postępy Biologii Komórki
  5. 1 Acta Neurobiologiae Experimentalis
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  1. 1 2012
  2. 2 2009
  3. 2 2008
  4. 1 2007
  5. 4 2006
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  1. 24 Pojda Z.
  2. 13 Machaj E. K.
  3. 8 Gajkowska A.
  4. 8 Oldak T.
  5. 5 Jastrzewska M.
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1
Content available Modyfikacja metody komór dyfuzyjnych umożliwiająca analizę klonalnego wzrostu ludzkich komórek krwiotwórczych
100%
Pojda Z.
Diagnostyka Laboratoryjna
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1984
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tom 20
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nr 5-6
2
Content available Erytropoeza w komorach dyfuzyjnych z ludzkim szpikiem wszczepianym dootrzewnowo myszom
100%
Pojda Z.
Diagnostyka Laboratoryjna
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1985
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tom 21
|
nr 2
3
Methodological contributions to studies on the sister chromatid exchanges of chromosomes in human lymphocytes
63%
Pojda Z. , Nawrowska M.
Genetica Polonica
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1977
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tom 18
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nr 1
4
Wymiany siostrzanych chromatyd - mechanizmy i zastosowanie
63%
Hubner H. , Pojda Z.
Postępy Biologii Komórki
|
1980
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tom 7
|
nr 3
5
Nowe metody badania wymian siostrzanych chromatyd
51%
Pojda Z. , Strozynski H. , Osiecka R.
Postępy Biologii Komórki
|
1976
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tom 3
|
nr 4
6
Zmiany populacji tymocytów pod wpływem wzrostu nowotworu u myszy
51%
Kawalec M. , Kawiak J. , Skorski T. , Pojda Z.
Postępy Biologii Komórki
|
1989
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tom 16
|
nr 1
7
Content available Podłoże bezsurowicze do hodowli mysich komórek krwiotwórczych
51%
Pojda Z. , Migdalska A. , Machaj E. K. , Dexter T. M.
Diagnostyka Laboratoryjna
|
1989
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tom 25
|
nr 3
8
Badanie potencjalnej przydatnosci klinicznej komorek macierzystych uzyskiwanych z krwi pepowinowej
45%
Pojda Z. , Machaj E. K. , Gajkowska A. , Oldak T. , Jastrzewska M.
Postępy Biologii Komórki. Suplement
|
2003
|
tom 21
127-137
9
Lack of migration and neurological benefits after infusion of umbilical cord blood cells in ischemic brain injury
45%
Zawadzka M. , Lukasiuk K. , Machaj E. K. , Pojda Z. , Kaminska B.
Acta Neurobiologiae Experimentalis
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2009
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tom 69
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nr 1
EN
The finding of stem/progenitor cells in postnatal bone marrow and umbilical cord blood, opens up a possibility of using stem cells to treat neurologic diseases. There is a controversy, whether intravenously administered human umbilical cord blood cells (HUCBC) migrate to the brain, differentiate and improve recovery after ischemia. In this study, 13 ×106 cells from non-cultured (non-committed) mononuclear HUCBC fraction were intravenously infused 1, 2, 3 or 7 days after a transient middle cerebral artery occlusion (MCAo) in adult rats. We found few human cells only in the ischemic area, localized mostly around blood vessels with few positive cells in the brain parenchyma. Timing of HUCBC delivery after ischemia or injection of Cyclosporin A at the time of delivery, had no effect on the number of human cells detected in the ischemic brain. Infusion of HUCBC did not reduce infarct volume and did not improve neurologic deficits after MCAo, suggesting that HUCBC failed to migrate/survive in the ischemic brain and did not provide significant neurological benefits.
10
Optimisation of transfection conditions of CD34plus hematopoietic cells derived from human umbilical cord blood
45%
Oldak T. , Kruszewski M. , Machaj E. K. , Gajkowska A. , Pojda Z.
Acta Biochimica Polonica
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2002
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tom 49
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nr 3
EN
Human umbilical cord blood is frequently used as a source of transplantable hematopoietic cells and more recently as a target of gene therapy - a new approach for treatment of various disorders. The aim of our study was optimisation of the transfection conditions of cord blood-derived CD34+ hematopoietic cells. Mononuclear cells fraction was isolated from cord blood samples by density gradient centrifugation. Subsequently, CD34+ hematopoietic cells were separated on immuno- magnetic MiniMACS columns. Pure population of CD34+ cells was incubated in a se­rum free medium supplemented with thrombopoietin, stem cell factor and Flt-3 ligand for 48 h and then transfected with plasmid DNA carrying the enhanced version of green fluorescent protein (EGFP) as a reporter gene. We studied the influence of vari­ous pulse settings and DNA concentrations on the transfection efficiency, measured by flow cytometry as the fluorescence of target cells due to the expression of EGFP. The optimal settings were as follows: 4 mm cuvette, 1600 iF, 550 V/cm, and 10 ug of DNA per 500 ul. With these settings we obtained a high transfection frequency (41.2%) without a marked decrease of cell viability. An increase of the pulse capaci­tance and/or of DNA concentration resulted in a greater electroporation efficiency, but also in a decrease of cell viability. In conclusion, the results described here allow one to recommend electroporation as an efficient method of gene delivery into CD34+ hematopoietic cells derived from human umbilical cord blood.
11
Nonhematopoietic stem cells of fetal origin - how much of today's enthusiasm will pass the time test?
45%
Pojda Z. , Machaj E. K. , Oldak T. , Gajkowska A. , Jastrzewska M.
Folia Histochemica et Cytobiologica
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2005
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tom 43
|
nr 4
12
Content available DNA damage in subpopulations of human lymphocytes irradiated with doses in the range of 0-1 Gy of X-radiation
38%
Wojewódzka M. , Machaj E. K. , Goździk A. , Iwaneńko T. , Ołdak T. , Kruszewski M. , Pojda Z.
Nukleonika
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2008
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tom Vol. 53, No. 4
145-149
EN
We compared three methods usually applied in biological dosimetry for estimation of radiation-induced DNA damage in human T and B lymphocytes: alkaline comet assay, micronucleus (MN) test and formation of histone gamma-H2AX foci. Human peripheral blood lymphocytes were fractionated using T cells and B cells isolation kits. Cells were irradiated with doses in the range of 0-1 Gy of X-rays. Induction of DNA damage was assessed by the standard alkaline comet assay, MN test and histone gammaH2AX foci immunofluorescence assay. Notwithstanding different end-points measured by the applied methods, all tests revealed a similar induction of DNA damage in B lymphocytes as compared with T lymphocytes. The results indicated that all three tests detect DNA damage with similar sensitivity, the lowest dose being approximately 0.3 Gy. The difference between irradiated and control cells was expressed as the ratio of the value obtained for irradiated cells (1 Gy) to that for control cells. The highest ratio was obtained for formation of gammaH2AX foci and was 6.2 for T and 13.8 for B lymphocytes, whereas those for comet assay and micronucleus test were 3.5; 3.6 and 5.6; 4.8, respectively.
13
Effect of substrate stiffness on differentiation of umbilical cord stem cells
38%
Witkowska-Zimny M. , Walenko K. , Walkiewicz A. E. , Pojda Z. , Przybylski J. , Lewandowska-Szumiel M.
Acta Biochimica Polonica
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2012
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tom 59
|
nr 2
EN
Tissue formation and maintenance is regulated by various factors, including biological, physiological and physical signals transmitted between cells as well as originating from cell-substrate interactions. In our study, the osteogenic potential of mesenchymal stromal/stem cells isolated from umbilical cord Wharton's jelly (UC-MSCs) was investigated in relation to the substrate rigidity on polyacrylamide hydrogel (PAAM). Osteogenic differentiation of UC-MSCs was enhanced on stiff substrate compared to soft substrates, illustrating that the mechanical environment can play a role in differentiation of this type of cells. These results show that substrate stiffness can regulate UC-MSCs differentiation, and hence may have significant implications for design of biomaterials with appropriate mechanical properties for regenerative medicine.
14
Preparatyka porcji krwi pępowinowej przeznaczonych do celów transplantacyjnych
38%
Oldak T. , Machaj E. K. , Gajkowska A. , Kruszewski M. , Jastrzewska M. , Pojda Z.
Diagnostyka Laboratoryjna
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2002
|
tom 38
|
nr 4
15
Differentiation potential of the fetal rat liver-derived cells
38%
Machaj E. K. , Grabowska I. , Gajkowska A. , Jastrzewska M. , Oldak T. , Moraczewski J. , Pojda Z.
Folia Histochemica et Cytobiologica
|
2005
|
tom 43
|
nr 4
16
Flow cytometric enumeration of CD34plus hematopoietic stem and progenitor cells in leukapheresis product and bone marrow for clinical transplantation: a comparison of three methods
38%
Gajkowska A. , Oldak T. , Jastrzewska M. , Machaj E. K. , Walewski J. , Kraszewska E. , Pojda Z.
Folia Histochemica et Cytobiologica
|
2006
|
tom 44
|
nr 1
17
Transcriptional activity of telomerase complex in CD34minus stem cells of cord blood in dependence of preparation time
32%
Stojko R. , Witek A. , Glogowska-Lingus J. , Mazurek U. , Chromy G. , Dworzecki T. , Bojdys-Szyndlar M. , Machaj K. , Pojda Z.
Folia Histochemica et Cytobiologica
|
2009
|
tom 47
|
nr 2
265-274
18
Porównanie dwóch metod izolacji DNA z krwi pępowinowej
32%
Oldak T. , Kruszewski M. , Machaj E. K. , Gajkowska A. , Klos M. , Kuczynska-Sicinska J. , Szczecina R. , Kruszewska H. , Pojda Z.
Diagnostyka Laboratoryjna
|
2001
|
tom 37
|
nr 2
19
Comparison of gene expression of mitogenic kinin path in adherent and non-adherent CD 34-stem cells using oligonucleotide microarrays
32%
Stojko R. , Witek A. , Glogowska J. , Mazurek U. , Chromy G. , Wilk K. , Witek L. , Bojdys-Szyndlar M. , Machaj K. , Pojda Z.
Folia Histochemica et Cytobiologica
|
2008
|
tom 46
|
nr 1
45-50
20
Contribution of stem cells to skeletal muscle regeneration
32%
Kawiak J. , Brzoska E. , Grabowska I. , Hoser G. , Streminska W. , Wasilewska D. , Machaj E. K. , Pojda Z. , Moraczewski J.
Folia Histochemica et Cytobiologica
|
2006
|
tom 44
|
nr 2
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