The paper presents the results of a study on differentiation of the morphological structure of soil and selected physical and chemical properties of soils in toposequence of the Opalenica Plain. The study was conducted in a 1200 m long transect running through a typical soil toposequence for the Polish Lowland, and therefore the results presented in this study can be extrapolated to similar geomorphological conditions of the area. On the basis of pedological cross-section, the following soil units were distinguished: PWspgl – Albic Luvisols (Arenic) with glossic properties, PAt – Albic Glossic Retisols (Loamic), PAsp – Albic Glossic Retisols (Aric, Arenic), PAspgg – Albic Glossic Retisols (Aric, Arenic, Oxyaquic), PWsggl – Albic Luvisols (Aric, Arenic, Stagnic) with glossic properties, PWgggl – Albic Luvisols (Aric, Loamic, Stagnic) with glossic properties, CZgg – Mollic Reductigleyic Eutric Gleysols (Aric, Loamic), CFt – Fluvic Phaeozems (Aric, Arenic). Each of these units has its own specific position in toposequence but the occurrence of Fluvic Phaeozems (Aric, Arenic) are associated with geogenetic processes of Mogilnica river. In this work, using a multiple regression analysis a statistically significant relationships between the position of the soils in relief and the terrain slopes and the organic carbon content in Ap horizon, the cation exchangeable capacity, the sum of exchangeable bases and the pH were obtained. Systematic variability of most soil properties of Ap horizon have shown two distances of spatial variation. The first concerns the systematic changes in shorter distance (from 132 to 344 m) and can be associated with differences in soil properties between separate soil units. The second distance of spatial correlation ranges from 431 m to 792 m, which testify to the fact that quantitative changes in the properties of soils are realized gradually and distinctly, together with the differentiation of the slope, over several separate cartographic units.