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EN
TPP enhanced the ability of nonactivated human mononuclear leucocytes (MNC) derived from the blood of young and aged donors, patients with coronary artery disease and patients with oral candidiasis, to induce neovascularization in the local graft versus host (GvH) reaction (H-LIA assay), diminished high activity of lymphocytes from rheumatoid artritic patients, but not influence the response of MNC of diabetic patients with proliferative retinopathy. Various fractions of mononuclear leucocytes were grafted intradermally into immunosuppressed (with cyclophosphamide) Balb/c mice. Recipients were then treated with PTT or saline. After 3 days the local GvH reaction was evaluated as the mean number of newly formed blood vessels surrounding the cell graft sites. CD4+ and CD8+ theophylline sensitive Fcy+ lymphocytes and monocytes responded to PTT by significant enhancement of their angiogenic activity.
PL
Oceniano wpływ TPP na aktywność angiogenną ludzkich leukocytów jednojądrzastych krwi obwodowej (MNC). Stwierdzono, że PTT stymuluje uwalnianie czynników angiogennych powodujących tworzenie się naczyń krwionośnych przez komórki MNC pochodzące od zdrowych dawców oraz od pacjentów ze schorzeniami geriatrycznymi, z chorobą niedokrwienną serca oraz pacjentów z kandydozą jamy ustnej, u których początkowa aktywność angiogenna jest obniżona. PTT obniża także patologicznie wysoką aktywność angiogenną komórek MNC pochodzących od pacjentów z reumatoidalnym zapaleniem stawów, ale nie zmienia takiej aktywności w przypadku komórek pochodzących od pacjentów z retinopatią cukrzycową. Komórkami odpowiadającymi na TPP są: monocyty Fcy+ oraz limfocyty Fcy+, CD4+ i CD8 + wrażliwe na teofilinę.
EN
The purpose of the study was estimating the viability and susceptibility effect of leukocytes isolated from cattle before and after the transportation in vitro on M. haemolytica leukotoxin (Lkt) cytotoxicity. 40 Simentaler heifers that were transported by truck a distance of 1700 km for 72 hours were used in the experiment. The material for the study was the blood (40 samples) collected on heparin directly before and after transportation. In relation to leukocytes the examination of susceptibility on cytotoxic effect of Lkt has been carried out with the use of MTT (microtitration assay) and the viability of leukocytes after 1, 2, 3 and 6 hour of incubation. The results obtained in the cell viability test did not show statistically significant differences (P≥0.05) in 1st and 2nd hour of incubation in leukocytes obtained from heifers before and after transportation. After the 1st hour of incubation the percentage of leukocyte viability was on a very high level and showed 87% in both groups of animals. The significantly lower cell viability values in comparison to leukocytes isolated from animals before the transportation was observed in the transported heifers from 3rd to 6th hour of incubation. The analysis of the results obtained by MTT test indicated statistically significant differences in the susceptibility of leukocytes for cytotoxic activity of Lkt. The average values of toxic activity of Lkt in relation to leukocytes isolated before and after transportation was 79% and 92% respectively. The lytic activity of Lkt for 50% of the cell population referred as 1 unit (1U) was observed in Lkt concentration 15 mg/ml (leukocytes before transportation) and 7.5 mg/ml (after the transportation). The increase of susceptibility of leukocytes isolated after transportation on the cytotoxic effect of leukotoxin suggest the significant influence of transporting stress on the increase of respiratory diseases caused by M. haemolytica strains.
EN
The reactivity of rat blood leukocytes after recurrent blood losses was examined. The blood samples were collected from the heart, three times in seven-day intervals. The volume of each sample was approximately 15% of the total blood volume. The functional changes in leukocytes were determined utilizing a test of radial segmentation of nuclei (RS) in mononuclear leukocytes and a test of Saccharomyces cerevisiae yeast phagocytosis. Our results demonstrate that sequential blood loss induced a decrease in the number of mononuclear cells indicating RS from 21.2% after 1st blood sampling up to 13% and 14% in following samplings: a decrease in number of phagocytic granulocytes from 49.5% after 1st blood sampling up to 41% and 39.3% after 2nd and 3rd sampling, respectively; and an increase in the number of phagocytic mononuclear blood cells from 8.5% after the 1st sampling up to 9.2% and 12.7% after the 2nd and 3rd blood samplings respectively. We affirm that this frequent blood loss modified the reactivity of blood leukocytes but did not change the WBC quantity in blood.
EN
Lactoferrin (LF) is an iron-binding protein from the transferrin family, present in mucosal secretions, granules of neutrophils and the serum of mammals. Many biological functions have been attributed to lactoferrin, including immunomodulatory and anti-inflammatory properties. Its presence has not been detected in poultry and its influence on their mechanisms of resistance has not been investigated. The aim of present in vitro study was to investigate the influence of bovine lactoferrin on the activity of chicken blood leukocytes. After isolation cells were cultured in complete RPMI - 1640 medium containing 0 (control), 2, 1, 0.5, 0.25 and 0.1 mg/ml of bovine LF, lymphocytes for 48 h and granulocytes for 18 h, respectively. Then the effect of LF on the activity of phagocytes (RBA and PKA tests) and lymphocytes (MTT test) was evaluated. The obtained results suggest that bovine lactoferrin has significant influence on the activity of chicken leukocytes in vitro. The use of LF increased the ability of chicken granulocytes for respiratory burst, however no stimulation of the killing activity was observed. Bovine lactoferrin also increased the proliferative response of lymphocytes T stimulated by ConA, whereas a similar effect on lymphocytes B stimulated by LPS was not noticed. The observed stimulation of the investigated parameters is encouraging for further research concerning the possibility of the use of LF as immunostimulant in poultry husbandry.
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