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Content available remote Tube Translocation of a Herniating Chain
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Translocation is modeled with the Rubinstein-Duke rules for hopping reptons along a one-dimensional lattice (tube). Identification of some coupled states guarantees a semi-periodicity of the process. The chain is driven through the pore by a bias potential promoting the transition of stored length in one direction. Accounting exactly for all allowed states the translocation time of polymers up to the 10-links length is determined. The crossover from reptation to faster dynamics through gradually allowing hernia creation and annihilation is found. Some computational details are presented.
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The PKB signaling pathway is essential for cell survival and the inhibition of apoptosis, but its functional mechanisms have not been fully explored. Previously, we reported that TPA effectively inhibited PKB activity and caused PKB degradation, which was correlated with the repression of PKB phosphorylation at Ser473. In this study, we focus on how PKB is regulated by TPA in gastric cancer cells. One of the TPA targets, PKCα, was found to mediate the inhibition of PKB phosphorylation and degredation caused by TPA. Furthermore, TPA induced the import of PKCα into the nucleus, where PKCα exerted an inhibitory effect on PKB expression and phosphorylation. As a result, cancer cell proliferation was arrested. Our study characterizes a novel function of PKCα in mediating the negative regulation of PKB by TPA, and suggests a potential application in the clinical treatment of gastric cancer.
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Steroid hormones in plants and in animals are very important for physiological and developmental regulation. In animals steroid hormones are recognized by nuclear receptors, which transcriptionally regulate specific target genes following binding of the ligand. In addition, numerous rapid effects generated by steroids appear to be mediated by a mechanism not depending on the activation of nuclear receptors. Although the existence of separate membrane receptors was postulated many years ago and hundreds of reports supporting this hypothesis have been published, no animal membrane steroid receptor has been cloned to date. Meanwhile, a plant steroid receptor from Arabidopsis thaliana has been identified and cloned. It is a transmembrane protein which specifically recognizes plant steroids (brassinosteroids) at the cell surface and has a serine/threonine protein kinase activity. It seems that plants have no intracellular steroid receptors, since there are no genes homologous to the family of animal nuclear steroid receptors in the genome of A. thaliana. Since the reason of the rapid responses to steroid hormones in animal cells still remains obscure we show in this article two possible explanations of this phenomenon. Using 1,25-dihydroxyvitamin D3 as an example of animal steroid hormone, we review results of our and of other groups concordant with the hypothesis of membrane steroid receptors. We also review the results of experiments performed with ovarian hormones, that led their authors to the hypothesis explaining rapid steroid actions without distinct membrane steroid receptors. Finally, examples of polypeptide growth factor that similarly to steroids exhibit a dual mode of action, activating not only cell surface receptors, but also intracellular targets, are discussed.
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The research aimed to assess the effect of bottom sediment on the content, bioaccumulation and translocation of heavy metals in maize biomass. The investigations were conducted in 2006-2007 as a pot experiment on light soil of the granulometric composition of weakly-loamy sand. The experimental design comprised 3 treatments: without sediment (I), a 5% sediment admixture (II) and a 10% sediment admixture to the soil (III). Bottom sediment was added to the soil in the first year of the investigations. The content of Zn, Cu, Ni. Pb, Cd and Cr was determined using the ICP-EAS method in the plant material after its dry mineralization and ash solution in HNO3. The uptake of the above-mentioned metals by maize was computed alongside their bioaccumulation and translocation coefficients. The effect of bottom sediment admixture on heavy metal concentrations in maize was determined to be varied, e.g. a 5% dose of sediment added to soil decreased the content of all the analyzed heavy metals in the biomass of maize aerial parts, whereas a 10% admixture increased the content of Cu, Ni, Pb and Cr. The values of bioaccumulation coefficients revealed that an admixture of both doses of bottom sediment led to a decreased accumulation of Zn, Cu, Cd, Cr and Ni (5% dose) in maize aerial biomass. Moreover, the plant more easily accumulated Zn, Cd and Cu than Cr, Ni or Pb. Permissible amounts of heavy metals in plants to be used as animal fodder were not exceeded in the maize biomass.
PL
Celem badań była ocena wpływu osadu dennego na zawartość, bioakumulację i translokację metali ciężkich w biomasie kukurydzy. Badania prowadzono w latach 2006-2007, w warunkach doświadczenia wazonowego, na glebie lekkiej o składzie granulometrycznym piasku słabogliniastego. Schemat doświadczenia obejmował 3 obiekty: bez osadu (I), z dodatkiem 5% osadu (II) i dodatkiem 10% osadu do gleby (III). Osad denny dodano do gleby w pierwszym roku badań. Zawartość Zn, Cu, Ni, Pb, Cd, Cr w materiale roślinnym oznaczono po suchej mineralizacji i roztworzeniu popiołu w HNO3. Obliczono wynos ww. metali przez kukurydzę oraz ich współczynniki bioakumulacji i translokacji. Stwierdzono niejednoznaczny wpływ dodatku osadu dennego na zawartość metali ciężkich w kukurydzy. Osad dodany w ilości 5% do gleby wpłynął na zmniejszenie zawartości wszystkich analizowanych metali w nadziemnej biomasie kukurydzy, natomiast 10% dodatek osadu na zwiększenie ich zawartości (Cu, Ni, Pb, Cr). Wartości współczynników bioakumulacji świadczą, że dodatek osadu dennego w obu dawkach spowodował zmniejszenie akumulacji Zn, Cu, Cd, Cr oraz Ni (dawka 5%) w biomasie nadziemnej kukurydzy, ponadto rośliny łatwiej akumulowały Zn, Cd i Cu niż Cr, Ni i Pb. W biomasie kukurydzy nie stwierdzono przekroczenia dopuszczalnych zawartości metali ciężkich przyjętych do oceny roślin pod względem ich przydatności paszowej.
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The aim of the study was to inventory potential habitats and localities of Eastern eggar Eriogaster catax (Linnaeus, 1758) on the route of expressway S5 Poznań-Wrocław at the Kaczkowo-Korzeńsko section. Insects from documented localities overlapping with the planned expressway were translocated to new locations, guaranteeing development and existence of this protected insect species.
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Celem pracy była inwentaryzacja potencjalnych siedlisk oraz stanowisk występowania barczatki kataks Eriogaster catax (Linnaeus, 1758) na trasie przebiegu drogi ekspresowej S5 Poznań-Wrocław na odcinku Kaczkowo-Korzeńsko. Owady ze stwierdzonych stanowisk kolidujących z planowaną trasą zostały przeniesione na nowe miejsca, gwarantujące rozwój i egzystencję tego chronionego gatunku owada.
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The clinical effects of twenty different chromosome anomalies in sheep are reviewed. It is concluded that not all anomalies give rise to reproductive problems or phenotypic variations. In particular, the centric fusion translocations appear not to have affected fertility and they have been helpful in determining the karyotype of the sheep.
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Output of Johannes Bobrowski, the author of four volumes of poetry − Sarmatische Zeit (1961)), Schattenland Ströome (1963), Wetterzeichen (1966) and Im Windgesträuch (1970), gives opportunity to have once again look on functions, which quoted places play in his poetry. It is worth to pay attention to his poetic imagination from the perspective of translocation of place in time and space by means of geopoetics’ instrumentarium. The space has become one of the most important factors for this poet. It is an example of writing down of human’s experiencing of place in the complex process of individual experiences connected with the process of shaping cultural, religious and social identity.
EN
The genetic basis of seedling and adult-plant leaf rust resistance was analysed in wheat lines CS 2A/2M 4/2 and CS 2D/2M 3/8, which are reference lines for the leaf rust resistance gene Lr28. Some seedlings of CS 2A/2M 4/2 were susceptible to Indian Puccinia triticina (Pt) pathotypes 77-1,77-2 and 77-5. These susceptible seedlings exhibited resistance at the adult-plant growth stage. In contrast, CS 2D/2M 3/8 showed resistance to all Pt pathotypes both at the seedling and adult-plant growth stages. The analysis of inheritance in the susceptible plants of CS 2A/2M 4/2 (CS 2A/2M 4/2 APR selection) and CS 2D/2M 3/8 against Pt 77-5 (the frequently occurring Pt pathotype from the Indian subcontinent), indicated that line CS 2D/2M 3/8 was fixed for a dominant gene, presumed to be Lr28, whereas line CS 2A/2M 4/2 was heterogeneous for Lr28. The adult-plant resistance in the CS 2A/2M 4/2 APR selection was conferred by an unknown recessive gene.
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