The aim of the studies was to establish relationships between flavonoids and elements important for human health. Therefore, total contents of flavonoids and phosphorus were determined by UV/Vis methods, flavonoids by HPLC, and Ca, Mg, Fe, Mn, Zn and Cu by FAAS in 68 infusions of medicinal herbs. Total flavonoids content in the aqueous extracts were in the range of 0.26 - 16.40 mg per 100 mL. The mean flavonoid contents (in mg per 100 mL of aqueous extract) were 2.24, 2.01, 1.83, 1.88 for rutin, myricetin, quercetin and kaempferol, respectively. The concentrations of Ca, Mg, P were determined in mg per 100 mL, and of Fe, Mn, Zn and Cu in μg per 100 mL. Total content of flavonoids was weakly correlated with quercetin (r = 0.41), kaempferol (r = 0.53), Cu (r = 0.43), and Ca (r = -0.30). Statistically significant correlations were also found among Cu, Ca, Mn, Zn and Fe. Cluster analysis grouped the studied herbs based on total flavonoids, also four flavonoids and essential elements contents, extracted from the whole population of herbs Sambuci flos, Betulae folium, and Sylibi mariani semen. Principal component analysis confirmed these findings and enabled identification of quercetin, kaempferol, Cu and Fe as the factors responsible for differentiation of the studied material.