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2014 | 99 | Complete | 331-338
Tytuł artykułu

Structural analysis and characterization of dextran produced by wild and mutant strains of Leuconostoc mesenteroides

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
•Dextran from Leuconostoc mesenteroides is structurally characterized.•Wild type (KIBGE-IB22) produces two exopolysaccharides called dextran and levan.•Levan production was suppressed by UV radiation.•UV irradiated mutant (KIBGE-IB22M20) remarkably produced dextran only.•Mutant produced higher yield of dextran is less fermentation time compared to wild.
EN
An exopolysaccharide known as dextran was produced by Leuconostoc mesenteroides KIBGE-IB22 (wild) and L. mesenteroides KIBGE-IB22M20 (mutant). The structure was characterized using FTIR, 1H NMR, 13C NMR and 2D NMR spectroscopic techniques, whereas surface morphology was analyzed using SEM. A clear difference in the spectral chemical shift patterns was observed in both samples. All the spectral data indicated that the exopolysaccharide produced by KIBGE-IB22 is a mixture of two biopolymers. One was dextran in α-(1→6) configuration with a small proportion of α-(1→3) branching and the other was levan containing β-(2→6) fructan fructofuranosyl linkages. However, remarkably the mutant only produced dextran without any concomitant production of levan. Study suggested that the property of KIBGE-IB22M20, regarding improved production of high molecular weight dextran in a shorter period of fermentation time without any contamination of other exopolysaccharide, could be employed to make the downstream process more feasible and cost effective on large scale.
Rocznik
Tom
99
Numer
Strony
331-338
Opis fizyczny
Twórcy
  • The Karachi Institute of Biotechnology & Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan
autor
  • The Karachi Institute of Biotechnology & Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan
autor
  • Dipartimento di Scienze Chimiche, Università degli Studi di Napoli Federico II, Via Cintia 4, I-80126 Napoli, Italy
  • The Karachi Institute of Biotechnology & Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan , ali_kibge@yahoo.com
  • Dipartimento di Scienze Chimiche, Università degli Studi di Napoli Federico II, Via Cintia 4, I-80126 Napoli, Italy
Bibliografia
  • 1. Aman, A.& Siddiqui, N.N.& Qader, S.A.U., "Characterization and potential applications of high molecular weight dextran produced by Leuconostoc mesenteroides AA1", Carbohydrate Polymer, vol. 87, 2012, p.910-915
  • 2. Bounaix, M.& Gabriel, V.& Morel, S.& Robert, H.& Rabier, P.& Ramaud-Siméon, M. et al., "Biodiversity of exopolysaccharides produced from sucrose by sourdough lactic acid bacteria", Journal of Agricultural and Food Chemistry, vol. 57, 2009, p.10889-10897
  • 3. De Vuyst, L.& Degeest, B., "Heteropolysaccharides from lactic acid bacteria", FEMS Microbiology Reviews, vol. 23, 1999, p.153-177
  • 4. Falconer, D.J.& Mukerjea, R.& Robyt, J.F., "Biosynthesis of dextrans with different molecular weight by selecting the concentration of Leuconostoc mesenteroides B-512FMC dextransucrase, the sucrose concentration, and the temperature", Carbohydrate Research, vol. 346, 2011, p.280-284
  • 5. Hassid, W.Z.& Abraham, S., "Methods in enzymology III", 1957, p.34-35
  • 6. Kang, H.K.& Kimura, A.& Kim, D., "Bioengineering of Leuconostoc mesenteroides glucansucrases that gives selected bond formation for glucan synthesis and/or acceptor-product synthesis", Journal of Agricultural and Food Chemistry, vol. 59, 2011, p.4148-4155
  • 7. Khan, T.& Park, J.K.& Kwon, J.H., "Functional biopolymers produced by biochemical technology considering applications in food engineering", Korean Journal of Chemical Engineering, vol. 24, 2007, p.816-826
  • 8. Kim, D.& Robyt, J.F., "Production, selection, and characteristics of mutants of Leuconostoc mesenteroides B–742 constitutive for dextransucrases", Enzyme and Microbial Technology, vol. 17, 1995, p.689-695
  • 9. Kim, D.& Robyt, J.F.& Lee, S.Y.& Lee, J.H.& Kim, M.Y., "Dextran molecular size and degree of branching as a function of sucrose concentration, pH, and temperature of reaction of Leuconostoc mesenteroides B-512FMCM dextransucrase", Carbohydrate Research, vol. 338, 2003, p.1183-1189
  • 10. Kitaoka, M.& Robyt, J.F., "Use of a microtiter plate screening method for obtaining Leuconostoc mesenteroides mutants constitutive for glucansucrase", Enzyme and Microbial Technology, vol. 22, 1998, p.527-531
  • 11. Kothari, D.& Tyagi, A.& Patel, S.& Goyal, A., "Dextransucrase from the mutant of Pediococcus pentosaceus (PPm) is more stable than the wild type", 3 Biotech, vol. 1, 2011, p.199-205
  • 12. Lowry, O.H.& Rosebrough, N.J.& Farr, A.L.& Randall, R.J., "Protein measurement with the folin phenol reagent", Journal of Biological Chemistry, vol. 193, 1951, p.265-275
  • 13. Maina, N.H.& Tenkanen, M.& Maaheimo, H.& Juvonen, R.& Virkki, L., "NMR spectroscopic analysis of exopolysaccharides produced by Leuconostoc citreum and Weisella confusa", Carbohydrate Research, vol. 343, 2008, p.1446-1455
  • 14. Nelson, N., "Photometric adaptation of the Somogyi method for the determination of glucose", Journal of Biological Chemistry, vol. 153, 1944, p.375-380
  • 15. Olvera, C.& Centeno-Leija, S.& Lopez-Munguía, A., "Structural and functional features of fructansucrases present in Leuconostoc mesenteroides ATCC 8293", Antonie van Leeuwenhoek, vol. 92, 2007, p.11-20
  • 16. Patel, S.& Goyal, A., "Isolation, characterization and mutagenesis of exopolysaccharide synthesizing new strains of lactic acid bacteria", The Internet Journal of Microbiology, 2010, p.8
  • 17. Patel, S.& Kothari, D.& Shukla, R.& Das, D.& Goyal, A., "Scale up of dextran production from a mutant of Pediococcus pentosaceus (spam) using optimized medium in a bioreactor", Brazilian Archives of Biology and Technology, vol. 54, 2011, p.1125-1133
  • 18. Piantini, U.& Sorensen, O.W.& Ernst, R.R., "Multiple quantum filters for elucidating NMR coupling networks", Journal of American Chemical Society, vol. 104, 1982, p.6800-6801
  • 19. Purama, R.K.& Goswami, P.& Khan, A.T.& Goyal, A., "Structural analysis and properties of dextran produced by Leuconostoc mesenteroides NRRL B-640", Carbohydrate Polymer, vol. 76, 2009, p.30-35
  • 20. Qader, S.A.U.& Aman, A., "Low molecular weight dextran: Immobilization of cells of Leuconostoc mesenteroides KIBGE HA1 on calcium alginate beads", Carbohydrate Polymer, vol. 87, 2012, p.2589-2592
  • 21. Rance, M.& Sørensen, O.W.& Bodenhausen, G.& Wagner, G.& Ernst, R.R.& Wüthrich, K., "Improved spectral resolution in COSY 1H NMR spectra of proteins via double quantum filtering", Biochemical and Biophysical Research Communications, vol. 117, 1983, p.479-485
  • 22. Sarwat, F.& Qader, S.A.U.& Aman, A.& Ahmed, N., "Production and characterization of a unique dextran from an indigenous Leuconostoc mesenteroides CMG713", International Journal of Biological Sciences, vol. 4, 2008, p.379-386
  • 23. Seymour, F.R.& Julian, R.L.& Jeanes, A.& Lamberts, B.L., "Structural analysis of insoluble d-glucans by Fourier-transform infrared difference-spectrometry: Correlation between structures of dextrans from strains of Leuconostoc mesenteroides and of d-glucans from strains of Streptococcus mutants", Carbohydrate Research, vol. 86, 1980, p.227-246
  • 24. Shingel, K.I., "Determination of structural peculiarities of dextran, pullulan and γ-irradiated pullulan by Fourier-transform IR spectroscopy", Carbohydrate Research, vol. 337, 2002, p.1445-1451
  • 25. Shukla, R.& Shukla, S.& Bivolarski, V.& Iliev, I.& Ivanova, I.& Goyal, A., "Structural characterization of insoluble dextran produced by Leuconostoc mesenteroides NRRL B-1149 in the presence of maltose", Food Technology and Biotechnology, vol. 49, 2011, p.291-296
  • 26. Siddiqui, N.N.& Aman, A.& Qader, S.A., "Mutational analysis and characterization of dextran synthesizing enzyme from wild and mutant strain of Leuconostoc mesenteroides", Carbohydrate Polymer, vol. 91, 2013, p.209-216
  • 27. Smith, M.R.& Zahnley, J.C., "Leuconostoc mesenteroides B-1355 mutants producing alternansucrases exhibiting decreases in apparent molecular mass", Applied and Environmental Microbiology, vol. 63, 1997, p.581-586
  • 28. Somogyi, M., "A new reagent for the determination of sugars", Journal of Biological Chemistry, vol. 160, 1945, p.61-68
  • 29. States, D.J.& Haberkorn, R.A.& Ruben, D.J., "A two-dimensional nuclear Overhauser experiment with pure absorption phase in four quadrants", Journal of Magnetic Resonance, vol. 48, 1982, p.286-292
  • 30. Uzochukwu, S.& Balogh, E.& Loefler, R.T.& Ngoddy, P.O., "Structural analysis by 13C nuclear magnetic resonance spectroscopy of glucan elaborated by gum-producing bacteria isolated from palm wine", Food Chemistry, vol. 73, 2001, p.225-233
  • 31. Van Geel-Schutten, G.H.& Faber, E.J.& Smit, E.& Bonting, K.& Smith, M.R.& Ten Brink, B. et al., "Biochemical and structural characterization of the glucan and fructan exopolysaccharides synthesized by the Lactobacillus reuteri wild-type strain and by mutant strains", Applied Environmental Microbiology, vol. 65, 1999, p.3008-3014
  • 32. Van Leeuwen, S.S.& Leeflang, B.R.& Gerwig, G.J.& Kamerling, J.P., "Development of a 1H NMR structural-reporter-group concept for the primary structural characterization of α-d-glucans", Carbohydrate Research, vol. 343, 2008, p.1114-1119
  • 33. Vedyashkina, T.A.& Revin, V.V.& Gogotov, I.N., "Optimizing the conditions of dextran synthesis by the bacterium Leuconostoc mesenteroides grown in a molasses-containing medium", Applied Biochemistry and Microbiology, vol. 41, 2005, p.361-364
  • 34. Vettori, M.H.P.B.& Franchetti, S.M.M.& Contiero, J., "Structural characterization of a new dextran with a low degree of branching produced by Leuconostoc mesenteroides FT045B dextransucrase", Carbohydrate Polymer, vol. 88, 2012, p.1440-1444
  • 35. Wang, T.& Deng, L.& Li, S.& Tan, T., "Structural characterization of a water-soluble (1→3)-α-d-glucan isolated from the Penicillium chrysogenum", Carbohydrate Polymer, vol. 67, 2007, p.133-137
Typ dokumentu
Bibliografia
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