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2014 | 99 | Complete | 331-338
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Structural analysis and characterization of dextran produced by wild and mutant strains of Leuconostoc mesenteroides

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•Dextran from Leuconostoc mesenteroides is structurally characterized.•Wild type (KIBGE-IB22) produces two exopolysaccharides called dextran and levan.•Levan production was suppressed by UV radiation.•UV irradiated mutant (KIBGE-IB22M20) remarkably produced dextran only.•Mutant produced higher yield of dextran is less fermentation time compared to wild.
An exopolysaccharide known as dextran was produced by Leuconostoc mesenteroides KIBGE-IB22 (wild) and L. mesenteroides KIBGE-IB22M20 (mutant). The structure was characterized using FTIR, 1H NMR, 13C NMR and 2D NMR spectroscopic techniques, whereas surface morphology was analyzed using SEM. A clear difference in the spectral chemical shift patterns was observed in both samples. All the spectral data indicated that the exopolysaccharide produced by KIBGE-IB22 is a mixture of two biopolymers. One was dextran in α-(1→6) configuration with a small proportion of α-(1→3) branching and the other was levan containing β-(2→6) fructan fructofuranosyl linkages. However, remarkably the mutant only produced dextran without any concomitant production of levan. Study suggested that the property of KIBGE-IB22M20, regarding improved production of high molecular weight dextran in a shorter period of fermentation time without any contamination of other exopolysaccharide, could be employed to make the downstream process more feasible and cost effective on large scale.
Opis fizyczny
  • The Karachi Institute of Biotechnology & Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan
  • The Karachi Institute of Biotechnology & Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan
  • Dipartimento di Scienze Chimiche, Università degli Studi di Napoli Federico II, Via Cintia 4, I-80126 Napoli, Italy
  • The Karachi Institute of Biotechnology & Genetic Engineering (KIBGE), University of Karachi, Karachi 75270, Pakistan ,
  • Dipartimento di Scienze Chimiche, Università degli Studi di Napoli Federico II, Via Cintia 4, I-80126 Napoli, Italy
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