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2015 | 62 | 2 | 303-309
Tytuł artykułu

Quinone- and nitroreductase reactions of Thermotoga maritima thioredoxin reductase

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The Thermotoga maritima NADH:thioredoxin reductase (TmTR) contains FAD and a catalytic disulfide in the active center, and uses a relatively poorly studied physiological oxidant Grx-1-type glutaredoxin. In order to further assess the redox properties of TmTR, we used series of quinoidal and nitroaromatic oxidants with a wide range of single-electron reduction potentials (E17, -0.49-0.09 V). We found that TmTR catalyzed the mixed single- and two-electron reduction of quinones and nitroaromatic compounds, which was much faster than the reduction of Grx-1. The reactivity of both groups of oxidants increased with an increase in their E17, thus pointing to the absence of their structural specificity. The maximal rates of quinone reduction in the steady-state reactions were lower than the maximal rates of reduction of FAD by NADH, obtained in presteady-state experiments. The mixed-type reaction inhibition by NAD+ was consistent with its competition for a NADH binding site in the oxidized enzyme form, and also with the reoxidation of the reduced enzyme form. The inhibition data yielded a value of the standard potential for TmTR of -0.31±0.03 V at pH 7.0, which may correspond to the FAD/FADH2 redox couple. Overall, the mechanism of quinone- and nitroreductase reactions of T. maritima TR was similar to the previously described mechanism of Arabidopsis thaliana TR, and points to their prooxidant and possibly cytotoxic role.
Wydawca

Rocznik
Tom
62
Numer
2
Strony
303-309
Opis fizyczny
Daty
wydano
2015
otrzymano
2015-03-06
poprawiono
2015-04-08
zaakceptowano
2015-04-28
(nieznana)
2015-06-22
Twórcy
  • Institute of Biochemistry of Vilnius University, LT-08662 Vilnius, Lithuania
  • Universite de Lorraine, Interactions Arbres-Microorganismes, UMR1136, F-54500 Vandoeuvre-les-Nancy, France
  • INRA, Interactions Arbres-Microorganismes, UMR1136, F-54280 Champenoux, France
  • Universite de Lorraine, Interactions Arbres-Microorganismes, UMR1136, F-54500 Vandoeuvre-les-Nancy, France
  • INRA, Interactions Arbres-Microorganismes, UMR1136, F-54280 Champenoux, France
  • Institute of Biochemistry of Vilnius University, LT-08662 Vilnius, Lithuania
Bibliografia
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Typ dokumentu
Bibliografia
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Identyfikator YADDA
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