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2014 | Vol. 23 | 111--117
Tytuł artykułu

Detection of immunogold markers in images obtained from transmission electron microscopy

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
In this paper a method is introduced which enables automatic detection of immunogold markers in transmission electron micrographs. Immunogold markers are used in electron microscopy to determine sub-cellular location of biological relevant macromolecules, such as proteins, lipids, carbohydrates, and nucleic acids. The proposed method combines image segmentation and feature localization approaches to improve accuracy of the immunogold markers detection in low contrast and highly textured image regions. A segmentation algorithm is intended in this study, which applies a flood-fill morphological operation. Accuracy of this method was evaluated by using electron microscopy images of human colorectal carcinoma cells. The experimental results show that the introduced method enables detection of immunogold markers with low false positive and false negative rates.
Wydawca

Rocznik
Tom
Strony
111--117
Opis fizyczny
Bibliogr. 13 poz., rys., tab.
Twórcy
autor
  • Department of Physiology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Zabrze, Poland
  • Department of Human Nutrition, School of Public Health, Medical University of Silesia, Zabrze, Poland
autor
  • Department of Human Nutrition, School of Public Health, Medical University of Silesia, Zabrze, Poland
autor
  • Department of Histology and Embryology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Zabrze, Poland
  • Department of Histology and Embryology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Zabrze, Poland
autor
  • Department of Histology and Embryology, School of Medicine with the Division of Dentistry, Medical University of Silesia, Zabrze, Poland
Bibliografia
  • [1] BRANDT S., HEIKKONEN J., ENGELHARDT P., Multiphase method for automatic alignment of transmission electron microscope images using markers. Journal of structural biology, 2001, Vol. 133 (1), pp. 10-22.
  • [2] BUŁDAK R. J., SKONIECZNA M., MATYSIAK N., WYROBIEC G., KUKLA M., MICHALSKI M., SWIRSKAKORCZALA K., Changes in subcellular localization of visfatin in human colorectal HCT-116 carcinoma cell line after cytochalasin B treatment. European Journal of Histochemistry, 2014, Vol. 58 (3), pp. 239-246.
  • [3] LEBONVALLET S., MENNESSON T., BONNET N., GIROD S., PLOTKOWSKI C., HINNRASKY J., PUCHELLE E., Semi-automatic quantitation of dense markers in cytochemistry. Histochemistry, 1991, Vol. 96 (3), pp. 245-250.
  • [4] LOWE D. G., Distinctive image features from scale-invariant keypoints. International journal of computer vision, 2004, Vol. 60 (2), pp. 91-110.
  • [5] MONTEIRO-LEAL L. H., TROSTER H., CAMPANATI L., SPRING H., TRENDELENBURG M. F., Gold finder: a computer method for fast automatic double gold labeling detection, counting, and color overlay in electron microscopic images. Journal of Structural Biology 141, 2003, pp. 228–239.
  • [6] NEJATBAKHSH R., KABIR-SALMANI M., DIMITRIADIS E., et al., Subcellular localization of L-selectin ligand in the endometrium implies a novel function for pinopodes in endometrial receptivity. Reprod Biol Endocrinol, 10:46, 2012, pp. 1-9.
  • [7] PŁACZEK B., Rough sets in identification of cellular automata for medical image processing. J. Med. Inf. Technol, 2013, Vol. 22, pp. 161-168.
  • [8] PŁACZEK B., Vehicles recognition using fuzzy descriptors of image segments. In Computer Recognition Systems 3, Springer Berlin Heidelberg, 2009, pp. 79-86.
  • [9] SALUJA R., JYOTI A., CHATTERJEE M., HABIB S., et al., Molecular and biochemical characterization of nitric oxide synthase isoforms and their intracellular distribution in human peripheral blood mononuclear cells. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research, 2011, Vol. 1813 (10), pp. 1700-1707.
  • [10] SHIMIZU Y., KABIR-SALMANI M., AZADBAKHT M., SUGIHARA K., SAKAI K., IWASHITA M., Expression and localization of galectin-9 in the human uterodome. Endocrine journal, 2008, Vol. 55 (5), pp. 879-887.
  • [11] SOILLE P., Morphological Image Analysis: Principles and Applications, Springer-Verlag, 1999, pp. 173-174.
  • [12] STARINK J. P., HUMBEL B. M., VERKLEIJ A. J., Three-dimensional localization of immunogold markers using two tilted electron microscope recordings. Biophysical journal, 1995, Vol. 68 (5), pp. 2171-2180.
  • [13] WANG R., POKHARIYA H., MCKENNA S. J., LUCOCQ J., Changes in subcellular localization of visfatin in human colorectal HCT-116 carcinoma cell line after cytochalasin B treatment. Recognition of immunogold markers in electron micrographs. Journal of structural biology, 2011, Vol. 176 (2), pp. 151-158.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.baztech-d63a4510-9880-42fe-b8ce-12427b35bd22
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