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2015 | 64 | 1 |
Tytuł artykułu

Recombinant MAG1 protein of Toxoplasma gondii as a diagnostic antigen

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The aim of this study was to evaluate the potential diagnostic usefulness of the full-length recombinant Toxoplasma gondii MAG1 protein by determining the levels of specific IgM and IgG antibodies in mouse and human sera obtained from individuals with acute and chronic toxoplasmosis. The obtained results revealed that IgG antibodies against MAG1 are a sensitive and specific marker of T. gondii infection since the protein was recognized by both mouse and human sera, 100% and 94.3%, respectively, rendering the full-length rMAG1 a prospective alternative for the polyvalent native antigen (TLA).
Słowa kluczowe
Wydawca
-
Rocznik
Tom
64
Numer
1
Opis fizyczny
p.55-59,fig.,ref.
Twórcy
  • Department of Immunoparasitology, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland
autor
  • Department of Immunoparasitology, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland
autor
  • Institute of Medical Biology, Polish Academy of Sciences, Lodz, Poland
autor
  • Department of Immunoparasitology, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland
autor
  • Department of Immunoparasitology, Faculty of Biology and Environmental Protection, University of Lodz, Lodz, Poland
Bibliografia
  • Di Cristina M., P. Del Porto, W. Buffolano, E. Beghetto, A. Spadoni, S. Guglietta, E. Piccolella, F. Felici and N. Gargano. 2004. The Toxoplasma gondii bradyzoite antigens BAG1 and MAG1 induce early humoral and cell-mediated immune responses upon human infection. Microbes Infect. 6(2): 164–171.
  • Dziadek B., J. Gatkowska, A. Brzostek, J. Dziadek, K. Dzitko, M. Grzybowski and H. Długońska. 2011. Evaluation of three recombinant multi-antigenic vaccines composed of surface and secretory antigens of Toxoplasma gondii in murine models of experimental toxoplasmosis. Vaccine 29(4): 831–840.
  • Ferguson D.J. and S.F. Parmley. 2002. Toxoplasma gondii MAG1 protein expression. Trends Parasitol. 18(11): 482.
  • Gatkowska J., E. Hiszczyńska-Sawicka, J. Kur, L. Holec and Długońska H. 2006. Toxoplasma gondii: an evaluation of diagnostic value of recombinant antigens in a murine model. Exp. Parasitol. 114(3): 220–227.
  • Guionaud C., A. Hemphill, M. Mevissen and F. Alaeddine. 2010. Molecular characterization of Neospora caninum MAG1, a dense granule protein secreted into the parasitophorous vacuole, and associated with the cyst wall and the cyst matrix. Parasitology. 137(11): 1605–1619.
  • Hester J., J. Mullins, Q. Sa, C. Mercier, M.F. Cesbron-Delauw and Y. Suzuki. 2012. Toxoplasma gondii antigens recognized by IgG antibodies between mice with and without active proliferation of tachyzoites in the brain during the chronic stage of infection. Infect. Immun. 80(10): 3611–3620.
  • Hiszczyńska-Sawicka E., M. Akhtar, G.W. Kay, L. Holec-Gasior, R. Bickerstaffe, J. Kur and M. Stankiewicz. 2010. The immune responses of sheep after DNA immunization with, Toxoplasma gondii MAG1 antigen-with and without co-expression of ovine interleukin 6. Vet. Immunol. Immunopathol. 136(3–4): 324–329.
  • Holec-Gąsior L., B. Ferra, D. Drapała, D. Lautenbach and J. Kur. 2012. A new MIC1-MAG1 recombinant chimeric antigen can be used instead of the Toxoplasma gondii lysate antigen in serodiagnosis of human toxoplasmosis. Clin. Vaccine Immunol. 19(1): 57–63.
  • Holec-Gąsior L. 2013. Toxoplasma gondii recombinant antigens as tools for serodiagnosis of human toxoplasmosis: current status of studies. Clin. Diagn. Lab. Immunol. 20(9): 1343–1351.
  • Holec-Gąsior L., B. Ferra, E. Hiszczyńska-Sawicka and J. Kur. 2014. The optimal mixture of Toxoplasma gondii recombinant antigens (GRA1, P22, ROP1) for diagnosis of ovine toxoplasmosis. Vet. Parasitol. 206(3–4): 146–152.
  • Holec L., E. Hiszczyńska-Sawicka, A. Gąsior, A. Brillowska-Dąbrowska and J. Kur. 2007. Use of MAG1 recombinant antigen for detection of Toxoplasma gondii infection in humans. Clin. Diagn. Lab. Immunol. 14(3): 220–225.
  • Kotresha D. and R. Noordin. 2010. Recombinant proteins in the diagnosis of toxoplasmosis. APMIS. 118: 529–542.
  • Montoya J.G. 2002. Laboratory diagnosis of Toxoplasma gondii infection and toxoplasmosis. J. Infect. Dis. 185 (suppl. 1): 73–82.
  • Pfrepper K. I., G. Enders, M. Gohl, D. Krczal, H. Hlobil, D. Wassenberg and E. Soutschek. 2005. Seroreactivity to and avidity for recombinant antigens in toxoplasmosis. Clin. Diagn. Lab. Immunol. 12: 977–982.
  • Sambrook J. and D.W. Russell. 2001. Molecular cloning: a laboratory manual, 3rd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press.
  • Xiao J., R.P. Viscidi, G. Kannan, M.V. Pletnikov, Y. Li, E.G. Severance, R.H. Yolken and L. Delhaes. 2013. The Toxoplasma MAG1 peptides induce sex-based humoral immune response in mice and distinguish active from chronic human infection. Microbes Infect. 15(1): 74–83.
Typ dokumentu
Bibliografia
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Identyfikator YADDA
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