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2002 | 49 | 1 |
Tytuł artykułu

Monitoring of urine nitric oxide [NO] related substrates and immunological competence in hematological malignancy

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The gag gene encoded protein, p24 of bovine leukemia virus (BLV), was cloned and ex­pressed as thioredoxin-6xHis-p24 protein in Escherichia coli. The bacterial cells carrying plasmid pT7THis-p24 expressed the protein of 38 kDa that was detected by immuno­blotting analysis using anti-p24 monoclonal antibodies and sera from BLV infected cattle and sheep. The purified p24 fusion protein was shown to be sensitive and specific for de­tection of BLV antibodies in the infected cattle.
Wydawca
-
Rocznik
Tom
49
Numer
1
Opis fizyczny
p.227-232,fig.
Twórcy
autor
  • Shimane Medical University, Izumo, Japan
autor
autor
autor
autor
Bibliografia
  • 1.Sagata, N., Yasunaga, T., Tsuzuku-Kawamura, J., Ohishi, K., Ogawa, Y. & Ikawa, Y. (1985) Complete nucleotide sequence of the genome of bovine leukemia virus: Its evolutionary relationship to other retroviruses. Proc. Natl. Acad. Sci. U.S.A. 82, 677-681.
  • 2.Deshayes, L., Levy, D., Parodi, A. & Levy, J. (1980) Spontaneous immune response of bovine leukemia virus infected cattle against five different viral proteins. Virology 136, 20-31.
  • 3.Bex, F., Brack, C., Mammerickx, M., Portetelle, D., Ghysdael, J., Cleuter, Y., Leclercq, M., Dekegel, D. & Burny, A. (1979) Humoral antibody response to bovine leukemia virus infection in cattle and sheep. Cancer Res. 39, 1118-1123.
  • 4.Brack, C., Portetelle, D., Mathot, S., Mammerickx, M. & Burny, A. (1984) Differential reactivity of cattle and sheep antisera to BLV gp51 epitopes. Leukemia Res. 8, 315-321.
  • 5.Doltz, G. & Moreno, E. (1999) Comparison of agar gel immunodiffusion test, enzyme-linked immunosorbent assay and western blotting for the detection of BLV antibodies. J. Vet. Med. B. 46, 551-558.
  • 6.Grover, Y. & Guillemain, B. (1992) An immunoblotting procedure for detection of antibodies against bovine leukemia virus in cattle. J. Vet. Med. B. 39, 48-52.
  • 7.Kwang, J. & Cutilp, R.C. (1992) Analysis of antibody response to ovine lentivirus by using viral gene products expressed in a procaryotic system. Biochem. Biophys. Res. Commun. 188, 20-27.
  • 8.Atkinson, B., Liu, Z. & Wood, C. (1992) Use of bacterial trpEfusion vectors to express and characterize the bovine immunodeficiency-like virus core protein. J. Virol. Methods 36, 35-49.
  • 9.Sambrook, J., Fritsch, E.F. & Maniatis, T (1989) Molecular Cloning: A Laboratory Manual, vol. 1, 2nd edn., Cold Spring Harbor Laboratory Press, Cold Spring Harbor.
  • 10.Rice, N., Stephens, A., Burny, A. & Gilden, N. (1984) The gag and pol genes of bovine leukemia virus: Nucleotide sequence analysis. Virology 142, 357-377.
  • 11.Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685.
  • 12.Kuzmak, J., Bicka, L., Grundboeck, J. & Kozaczynska, B. (1993) Application of western blot method for detection of viral proteins of bovine leukemia virus. Med. Vet. 49, 471-474.
  • 13.Bunger, I., Khalaf, C., Cripe, C. & Rimpler, M. (1994) Detection of antibodies against bovine leukaemia virus in milk and serum samples by immunoblotting. Deutsch. Tieraerztl. Wschr. 101, 402-405.
  • 14.Kittelberger, R., Laybourn, B., Biack, D., Penrose, M., Reichel, M., Motha, J., Molloy, J. & Merza, M. (1996) Evaluation of electrophoretic immunoblotting for the detection of antibodies against the bovine leukosis virus in cattle. J. Virol. Methods 61, 7-22.
  • 15.Valker, P., Molloy, J. & Rodwell, B. (1987) A protein immunoblot test for detection of bovine leukemia virus p24 antibody in cattle and experimentally infected sheep. J. Virol. Methods 15, 201-211.
  • 16.Heeney, J., Valli, V. & Montesanti, J. (1988) Alteration in humoral immune response to bovine leukaemia virus antigens in cattle with lymphoma. J. Gen. Virol. 69, 659-666.
  • 17.Portetelle, D., Bruck, C., Mammerickx, M. & Burny, A. (1980) In animals infected with BLV antibodies to envelope glycoprotein gp51 are directed against the carbohydrate moiety. Virology 105, 223-233.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-article-c9cc583a-1147-4e1d-a52d-b7fa3e283480
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