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2005 | 23 | 2 |
Tytuł artykułu

DNA fingerprinting analysis of rabbits from lines divergently selected for high and low open-field activity

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Języki publikacji
The aim of the study was to analyse whether a successful divergent selection for open-field activity (OFA) in rabbits has resulted in differences in DNA fingerprinting pattern and genetic parametres of diversity. We also scanned DNA fingerprinting profiles for searching minisatellite alleles potentially linked to genes determining the trait under selection. Rabbits derived from the 8th generation of the lines selected for high (H) or low (L) locomotor OFA were profiled for DNA fingerprinting using HinfI enzyme and 33.6 multilocus probe. The H and L lines represent different, i.e. active and passive coping strategies in a novel situation.Selection for H or L locomotor OFA did not affect significantly the molecular parametres of genetic diversity. However, the analysis of band patterns for individual and pooled DNA fingerprints revealed a specific band for the L line at 15 kbp. No specific bands for the H line were detected. The results presented provide evidence of a possible linkage between minisatellites and OFA in rabbits and demonstrate that studies on the H and L lines may give rise to a new strategy in animal breeding and selection for traits related to animal welfare (locomotion and fear-related emotional behavior) and to the study of genetic background of hyperactivity disorders, e.g. ADHD.
Celem badań było określenie, czy selekcja królików na aktywność w otwartym polu spowodowała zróżnicowanie we wzorze DNA fingerprinting, a także czy efektem tej selekcji jest zmiana genetycznych parametrów zmienności na poziomie molekularnym. Ponadto wzory DNA fingerprinting analizowano pod względem obecności specyficznych alleli minisatelitarnych, które potencjalnie mogą być sprzężone z genami determinującymi selekcjonowaną cechę. Profilowanie DNA fingerprinting przeprowadzono na ósmym pokoleniu królików selekcjonowanych na wysoką (H) i niską (L) aktywność w otwartym polu, z użyciem enzymu HinfI i wielopozycyjnej sondy 33.6. Rozbieżna selekcja nie spowodowała znaczącej zmiany w genetycznych parametrach zmienności. Jednakże analiza indywidualnych i reprezentatywnych wzorów prążkowych pozwoliła na identyfikację w linii L specyficznego allelu minisatelitarnego o długości 15 tysięcy par zasad. Nie wykryto specyficznych alleli u osobników linii H.
Opis fizyczny
  • Polish Academy of Sciences, Jastrzebiec, 05-552 Wolka Kosowska, Poland
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