Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2003 | 45 | 2 |
Tytuł artykułu

In vitro propagation of Centaurea rupestris L.

Warianty tytułu
Języki publikacji
A rapid clonal propagation method was developed for Centaurea rupestris L., a Balkan Apennine endemic which contains a flavonoid with strong antiphytoviral, antibacterial and antifungal activity. Shoots from aseptically germinated seeds were used for culture initiation. The highest multiplication rate, 11.88 shoots per explant, was achieved in 4 weeks-culture-period in the third subculture on MS medium supplemented with 1 µM 6-benzylamino-purine and 2.9 µM gibberellic acid. The best rooting of excised shoots was achieved on half-strength MS medium supplemented with 3 µM indole-3-butyric acid. Rooted plantlets were transferred to potting soil and acclimatized to outdoor conditions.
Opis fizyczny
  • University of Zagreb, Marulicev trg 20-II, 10000 Zagreb, Croatia
  • Duncan DB. 1955. Multiple range and multiple F-tests. Biometrics 11: 1-42.
  • George EF, and Sherrington PD. 1984. Plant propagation by tissue culture. Exegetics Ltd., Eversley.
  • Hegi G. 1931. Ilustrierte Flora von Mitteleuropa, vol. VI/2. A. Pichler’s Witwe & Son, Wien.
  • Murashige T, and Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473-497.
  • Pavletić Z, and Trinajstić I. 1983. A study of taxonomical relations between the species Centaurea rupestris L. and C. fritschii Hayek, and their spontaneous hybrid C. x sordida Willd. (Asteraceae, sect. Acrocentron Cass. 1926). Acta Botanica Croatica 42: 137-143 (in Croatian with English summary).
  • Pevalek-Kozlina B. 1998. In vitro propagation of Centaurea ragusina L., a Croatian endemic species. Acta Biologica Cracoviensia Series Botanica 40: 21-24.
  • Pevalek-Kozlina B, Kostović V, and Slade D. 1997. In vitro propagation of Fibigia triquetra (DC.) Boiss., a rare stenoendemic species. Plant Cell Tissue and Organ Culture 51: 141-143.
  • Rusak G, Krajačić M, and Pleše N. 1997. Inhibition of tomato bushy stunt virus infection using a quercetagetin flavonoid isolated from Centaurea rupestris L. Antiviral Research 36: 125-129.
  • Rusak G, Robinson N, and Pepeljnjak S. 2002. Antibacterial and antifungal activity of extracts and quercetagetin derívate isolated from Centaurea rupestris L. (Asteraceae). Acta Biologica Cracoviensia Series Botanica 44: 169-174.
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.