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2009 | 18 | 3 | 399-404
Tytuł artykułu

Assessment of aging in Saccharomyces cerevisiae yeast mutants using microscopy techniques

Autorzy
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Different microscopy techniques were used to assess the aging of Saccharomyces cerevisiae yeast cells in stationary culture. Live/Dead staining was found to be as effective as viability tests at determining viability of yeast cells. Cells of dismutase-deficient sod1 and sod2 yeast mutants age faster than wild-type strains. Changes in appearance and decreasing in diameter size of the aging cells was observed using the Nomarski technique. The results of the fluorescent staining with Redox Sensor Red and mBCl suggest a disturbance of redox homeostasis in the cells, while vacuole staining with CellTracker Blue CMAC indicates cytoplasmic vacuolization. Yeast cell staining as carried out in this study can be used as a fast preliminary test, making it possible to determine changes taking place in the cell during aging.
Wydawca
-
Rocznik
Tom
18
Numer
3
Strony
399-404
Opis fizyczny
p.399-404,ref.
Twórcy
autor
  • Department of Agricultural Sciences in Zamosc, University of Life Sciences in Lublin, Department of Agricultural Sciences in Zamosc, Szczebrzeska 102, 22-400 Zamosc, Poland
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-article-45b396f4-39c9-45fb-a638-95ec7cb67bb4
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