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2006 | 15 | 3 | 435-444
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Antioxidant and selenium status of laying hens fed with diets supplemented with selenite or Se-yeast

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The experiment was designed to investigate the effects of feed supplementation with selenite or selenized yeast on parameters of antioxidant and selenium status of laying hens. Hens of laying breed Shaver Starcross 288 were randomly divided at the day of hatching into 4 groups and fed for 9 months on diets which differed only in amounts or forms of selenium supplemented. Group 1 was fed the basal diet (BD) with native Se content 0.1 DM. Groups 2 and 3 were fed the BD diets supplemented with equivalent Se dose 0.4 DM of either sodium selenite or Se-yeast, respectively. The diet for group 4 was supplemented with Se-yeast at Se dose 0.9 DM. The activities of glutathione peroxidase (GPx) in blood and tissues of liver, kidney and duodenal mucosa were significantly increased by Se supplementation, but no differences due to form or dose of Se were observed. Both Se sources resulted in significant reduction of superoxide dismutase (SOD) activity in erythrocytes. Malondialdehyde (MDA) content in kidney tissue was reduced by both Se sources, but its production in liver tissue was inhibited by Se-yeast only. Selenium supplementation did not influence the levels of MDA and -SH groups in plasma. Altrough both Se significantly raised Se concentrations in blood and tissues of liver, kidney, spleen, hearth and duodenal mucosa, significant Se deposition into muscles appeared in hens given Se-yeast only. The presented results suggest that Se-yeast is more effective in maintenance of antioxidant and selenium status of laying hens than selenite.
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  • Slovak Academy of Sciences, Soltesovej 4, 040 01 Kosice, Slovak Republic
  • Arai T., Sugawara M., Sako T., Motoyoshi S., Shimura T., Ttsutsui N., Konno T., 1994. Glutathione peroxidase activity in tissues of chickens supplemented with dietary selenium. Comp. Biochem. Physiol. 107, 245-248
  • Arthur J.R., Boyne R., 1985. Superoxide-dismutase and glutathione-peroxidase activities in neutrophils from selenium deficient and copper deficient cattle. Life Sci. 36, 1569-1575
  • Balogh K., Weber M., Erdélyi M., Mézes M., 2004. Effect of excess selenium supplementation on the glutathione redox system in broiler chicken. Acta Vet. Hung. 52, 403-411
  • Behne D., Kyriakopoulos A., 2001. Selenium-containing proteins in mammals and other forms of life. Rev. Physiol. Biochem. Pharmacol. 145, 1-46
  • Behne D., Wolters W., 1983. Distribution of selenium and glutathione peroxidase in the rat. J. Nutr. 113, 456-461
  • Bradford M., 1976. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254
  • Daniels L.A., 1996. Selenium metabolism and bioavailability. Biol. Tr. Elem. Res. 54, 185-199
  • Daun C., Lundh T., Onning G., Akesson B., 2004. Separation of soluble selenium compounds in muscle from seven animal species using size exclusion chomatography and inductively coupled plasma mass. J. Anal. Atom. Spectrom. 19, 129-134
  • Edens F.W., Gowdy K.M., 2004. Selenium sources and selenoproteins in practical poultry production. In: T.P. Lyons, K.A. Jacques (Editors). Proceedings of Alltech’s 20th Annual Symposium “Nutritional Biotechnology in the Feed and Food Industries”. Nottingham University Press, pp. 35-55
  • Ellman G.L., 1958. Tissue sulfhydryl groups. Arch. Biochem. Biophys. 82, 70-77
  • Jo C., Ahn D.U., 1998. Fluorometric analysis of 2-thiobarbituric acid reactive substances in turkey. Poultry Sci. 77, 475-480
  • Kobayashi Y., Ogra Y., Suzuki K.T., 2001. Speciation and metabolism of selenium injected with 82Se-enriched selenite and selenate in rats. J. Chromatogr. B. Biomed. Sci. Appl. 760, 73-81
  • Kuricová S., Boldižárová K., Grešáková Ľ., Levkut M., Leng Ľ., 2003. Chicken selenium status when fed a diet supplemented with Se-yeast. Acta Vet. Brno 72, 339-346
  • Paglia D.E., Valentine W.N., 1967. Studies on quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J. Lab. Med. 70, 158-169
  • Pavlata L., Illek J., Pechová A., 2001. Blood and tissue selenium concentration in calves treated with inorganic or organic selenium compounds - A comparison. Acta Vet. Brno 70, 19-26
  • Rayman M.P., 2004. The use of high-selenium yeast to raise selenium status: how does it measured up? Brit. J. Nut. 92, 557-573
  • Rodriguez E.M., Sanz M.T., Romero C.D., 1994. Critical study of fluorometric determination of selenium in urine. Talanta 12, 2025-2031
  • Schrauzer G.N., 2000. Selenomethionine: A review of its nutritional significance, metabolism and toxicity. J. Nutr. 130, 1653-1656
  • Sies H., Klotz L.O., Sharov V.S., Assmann A., Briviba K., 1998. Protection against peroxynitrite by selenoproteins. Z. Naturforsch. 53, 228-232
  • Surai P.F., 2002. Selenium in poultry nutrition 1. Antioxidant properties, deficiency and toxicity. World Poultry Sci. J. 58, 333-347
  • Tirosh O., Reznick A.Z., 2000. Chemical bases and biological relevance of protein oxidation. In: C.K. Sen, L. Packer, O.O.P. Hanninen (Editors). Handbook of Oxidants and Antioxidants in Exercise. Elsevier, Amsterdam, pp. 89-114
  • Toyoda H., Himeno S., Imura N., 1990. Regulation of glutathione peroxidase mRNA level by dietary selenium manipulation. J. Nutr. 122, 1620-1626
  • Zagrodzki P., Nicol F., McCoy M.A., Smyth J.A., Kennedy D.G., Beckett G.J., Arthur J.R., 1998. Iodine deficiency in cattle: compensatory changes in thyroidal selenoenzymes. Res. Vet. Sci. 64, 209-211
  • Zuberbuehler C.A., Messikommer R.E., Arnold M.M., Forrer S.R., Wenk C., 2005. Effects of selenium depletion and selenium repletion by choice feeding on selenium status of young and old laying hens. Physiol. Behav. 87, 430-440
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