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2000 | 05 | 4 | 423-431
Tytuł artykułu

The detection and characterization of rat protein recognizing DNA damaged by N-acetoxy-acetylaminofluorene

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Proteins recognizing DNA damaged by the chemical carcinogen N- acetoxy-acetylaminofluorene (AAAF) were analyzed in nuclear extracts from rat tissues, using a 36 bp oligonucleotide as the substrate and an electrophoretic mobility shift assay. Two major proteins that form complexes with DNA damaged by AAAF were detected; one of them also bound DNA damaged by cis-diammine-dichloroplatinum. The complex specific for AAAF-damaged DNA contained protein loosely attached to nuclear components. It was extracted with 0.1 M NaCl. The amount of this protein was estimated at about 105 copies per liver cell nucleus, and its probable size was about 42 kDa as detected by the Southwestern blotting assay. Its affinity for DNA damaged by AAAF was ~10-fold higher than that for undamaged DNA. Analogous AAAF- DDB (damaged-DNA-binding) proteins were also detected in extracts from rat brain, testis and kidney tissue. The levels of such proteins were not affected in rats treated with the carcinogen 2-acetylaminofluorene.
Wydawca
-
Rocznik
Tom
05
Numer
4
Strony
423-431
Opis fizyczny
p.423-431,fig.,ref.
Twórcy
  • Center of Oncology, 44-100 Gliwice, Poland
autor
autor
Bibliografia
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  • 9. Rzeszowska-Wolny, J. and Widłak, P: Damaged DNA-binding proteins: recognition of N-acetoxy-acetylaminofluorene-induced DNA adducts. Acta Biochim. Polon. 46 (1999) 173-180.
  • 10. Łanuszewska, J., Cudak, A., Rzeszowska-Wolny, J. and Widłak, P. Detection of damage-recognition proteins from human lymphocytes. Acta Biochim. Polon. 47 (2000) 443-450.
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  • 14. Lang, M. C. E, Freund, A. M., de Murcia, G., Fuchs, R. P. P. and Daune, M. P. Unwinding of supercoiled ColE1-DNA after covalent binding of the ultimate carcinogen N-acetoxy-N-2-acetylaminofluorene and its 7-iodo derivative. Chem. Biol. Interactions 28 (1979) 171-180.
  • 15. Leng, M. DNA bending induced by covalently bound drugs; gel electrophoresis and chemical probe studies. Biophys. Chem. 35 (1990) 155-163.
Typ dokumentu
Bibliografia
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Identyfikator YADDA
bwmeta1.element.agro-article-436d8142-e74e-4bd7-8087-d6a9ae6cb712
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