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Induced androgenesis of Capsicum frutescens L.

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The aim of the research was to make a preliminary determination of the effectiveness of the in duc tion of haploids in Capsicum frutescens L. In order to induce androgenesis red and yellow fruit forms of species were used, each bred by the re searchers on their own. The experiment was per formed in October. An ther cultures were conducted according to a modified me - thod developed by Dumas et al. (1981) for C. annuum L. The anthers were laid on CP medium con taining 0.01 mg·dm⁻³ 2.4-D and 0.01 mg·dm⁻³ kinetin, with the addition of 0.5 g·dm⁻³ of activated carbon and 5 mg×dm⁻³ of silvernitrate, solidified with 8 g·dm⁻³ of agar. The cul tures were in cu bated in the dark at 35 deg C for 8 days. Next they were trans ferred to 25 deg C under a 12-hour photoperiod. After 14 days of induction, anthers were trans ferred to R₁ medium supplemented with 0.1 mg·dm⁻³ kinetin. Obtained embryos were subsequently transplanted onto V₃ hormone-free me dium and well growing plants were planted in green houses. The efficiency of androgenesis for both C. frutescens L. forms was relatively low and it did not exceed 5 %. The ploidy level of the result ing plants was determined by flow-cytometric analysis. The regenerants con sisted of about equal numbers of haploids and diploids. Additionally, among plants regenerated from anthers of yellow fruit forms, two mixoploids were observed.
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  • University of Technology and Agriculture, Bernardynska 6/8, 85-021 Bydgoszcz, Poland
  • Alegbejo M. D., Orakwue F. C. 2002. Characteristics of some pepper cultivars commonly grown in Nigeria. Capsicum and Egg plant News letter, 21: 22-24.
  • Carvalho S. I. C., Bianchetti L. B., Henz G. P. 2003. Germplasm collection of Capsicum spp. main tained by EMBRAPA Hortalicas. Capsicum and Egg plant News letter, 22: 17-20.
  • Dumas de Vaulx R., Chambonnet D., Pochard E. 1981. Culture in vitro d’anthéres de piment (Capsi cum annuum L.) amélioration des taux d’obtention de plantes chez différents génotypes par des traitements á +35 0C. Agronomie, 1: 859-864.
  • Dolcet-Sanjuan R., Claveria E., Huerta A. 1997. Androgenesis in Capsicum annuum L.: effect of carbohydrate and carbon dioxide enrichment. J. Amer. Soc. Hort. Sci., 122: 468-475.
  • Galbraith D. W., Harkins K. R., Mad dox J. M., Ayres N. M., Sharma D. P., Firoozabady E. 1983. Rapid flow cytometry analysis of the cell cycle in intact plant tissues. Sci ence, 220: 1049-1051.
  • Kristiansen K., Andersen S.B. 1993. Effects of do norplant, temperature, photoperiod and age on an ther culture response of Capsicum annuum L. Euphytica, 67: 105-109.
  • Li C. L., Jiang Z. R. 1990. A new sweet pep per cultivar, Haihua 3, de vel oped by an ther cul ture. Acta Hort. Sinica, 17: 39-44.
  • Maheshwari S. C., Rashid A., Tyagi A. K. 1982. Haploids from pollen grains – retrospect and pros pect. Amer. J. Bot., 69: 865-879.
  • Matsubara S., Yamamoto M., Jo M. H., Murakami K. 1998. Embryoid and callus formation from microspores by an ther culture from July to November in pepper (Capsicum annuum L.). Scientific Reports of the Faculty of Agriculture Okayama Uni ver sity, 87: 117-122.
  • Mitykó J., Andrásfalvy A., Csillery G., Fári M. 1995. An ther-culture response in different genotypes and F1 hybrids of pepper (Capsicum annuum L.). Plant Breed., 114: 78-80.
  • Mitykó J., Fári M. 1997. Problems and results of doubled haploid plant production in pepper (Cap sicum annuum L.) via an ther- and microspore culture. Acta Hort., 447, ISHS 1997.
  • Nervo G., Carannante G., Azzimonti M. T., Rotino G. L. 1995. Use of anther culture method in pep per breeding: factors affecting plant lets production. M. Terzi et al. (eds.). Current Issues in Plant Molecular and Cellular Biology, 155-160.
  • Nowaczyk L., Nowaczyk P. 2004a. Soft-flesh fruits of Capsicum frutescens L. as a raw material for processing. Annales UMCS, Sec. E, 59, 4: 1823-1828.
  • Nowaczyk L., Nowaczyk P. 2004b. Hot and sweet soft-flesh hybrids be tween Capsicum annuum L. and Capsicum frutescens L. Acta Hort. (in press)
  • Nowaczyk P., Olszewska D., 1999. The differences of interspecific hybrid seeds size within Capsi cum genus. Acta Biol. Crac., Vol. 41, 1,55.
  • Regner F. 1996. Anther and microspore culture in Capsicum. In Vitro Haploid Production in Higher Plants, 3: 77-89.
  • Sibi M., Dumas de Vaulx R., Chambonnet D. 1979. Obtention de plantes haploides par androgenése in vitro chez le piment (Capsicum annuum L.). Ann. Amélior. Plantes, 29: 583-606.
  • Sreelathakumary I., Rajmony L. 2003. Variability, heritability and genetic advance in bird pepper (Capsicum frutescens L.). Capsicum and Egg plant News let ter, 22: 51-54.
  • Vagera J. 1990. Pepper (Capsicum ssp.): In Vitro Induction of Hap loids. Biotech. In Agri. Forestry, 12: 374-392.
  • Vagera J., Havranek P. 1985. In vitro induction of androgenesis in Capsicum annuum L. and its ge netic aspects. Biol. Plant., 27: 10-21.
  • Venczel G., Mitykó J. 1995. In vitro haploid research and practical utilisation in pepper (Capsicum annuum L.) preservation. Zöldségtermesztési Kutató Intézet Bulletinje, 27: 145-156.
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