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2001 | 45 | 2 |
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In vitro influence of methisoprinol on lymphocyte proliferation in dogs

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The application of immunostimulators in dogs offers a novel approach to induce or modulate the mechanisms of protection against infectious diseases. In the present studies we evaluated in vitro influence of different concentrations of synthetic product - methisoprinol on the proliferative response of blood lymphocytes stimulated by pokeweed mitogen (PWM), concanavaline A and lipopolisaccharide (LPS) in dogs. The concentrations of methisoprinol between 1 to 50 μg/ml increased the mitogen-induced proliferation rate of canine T and B lymphocytes. Enhanced response to mitogens was observed both in 1-2 and 6-8 years old dogs, but the best results were observed in older animals. The results suggested that methisoprinol demonstrated a stronger effect on the lymphocytes isolated from older animals with the lower proliferative responses.
Opis fizyczny
  • University of Warmia and Mazury in Olsztyn, Olsztyn, Poland
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