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1998 | 45 | 1 |
Tytuł artykułu

Beta-carotene enhances the recovery of lymphocytes from oxidative DNA damage

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Epidemiological studies have revealed a strong correlation between high intake of fruit and vegetables and low incidence of certain cancers. Micronutrients present in these foods are thought to decrease free radical attack on DNA and hence protect against mutations that cause cancer, but the fine details of the causal mechanism have still to be elucidated. Whether dietary factors can modulate DNA repair - a crucial element in the avoidance of carcinogenesis - is an intriguing question that has not yet been satisfactorily answered. In order to investigate the effects of β-carotene on oxidative damage and its repair, volunteers were given a single 45 mg dose and lymphocytes taken before and after the supplement were treated in vitro with H2O2. DNA strand breaks and oxidised pyrimidines were measured at intervals, to monitor the removal of oxidative DNA damage. We found inter-individual variations in response. In cases where the baseline plasma β-carotene concentration was high, or where supplementation increased the plasma concentration, recovery from oxidative damage (i.e. removal of both oxidised pyrimidines and strand breaks) was relatively rapid. However, what seems to be an enhancement of repair might in fact represent an amelioration of the continuing oxidative stress encountered by the lymphocytes under in vitro culture conditions. We found that culture in a 5% oxygen atmosphere enhanced recovery of lymphocytes from H2O2 damage.
Wydawca
-
Rocznik
Tom
45
Numer
1
Opis fizyczny
p.183-190,fig.
Twórcy
autor
  • Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, U.K.
autor
autor
Bibliografia
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  • 3. Negri, E., La Vecchia, C., Franceschi, S., D'Avanzo, B. & Parazzini, F. (1991) Vegetable and fruit consumption and cancer risk. Int. J. Cancer 48, 350-354.
  • 4. Byers, T. & Perry, G. (1992) Dietary carotenes, vitamin C, and vitamin E as protective antioxi­dants in human cancers. Annu. Rev. Nutr. 12, 139-159.
  • 5. Ziegler, R.G. (1989) A review of epidemiologi­cal evidence that carotenoids reduce the risk of cancer. J. Nutr. 119, 116-122.
  • 6. Gerster, H. (1993) Anticarcinogenic effect of common carotenoids. Int J. Vit. Nutr. Res. 63, 93-121.
  • 7. Ames, B.N., Shigenaga, M.K. & Hagen, T.M. (1993) Oxidants, antioxidants and the degen­erative diseases of ageing. Proc. Natl Acad. Act U.S.A 90. 7915-7922.
  • 8. Duthie, S.J., Ma, A., Ross, M.A. & Collins, A.R. (1996) Antioxidant supplementation de­creases oxidative DNA damage in human lym­phocytes. Cancer Res. 56, 1291-1295.
  • 9. The Alpha-Tocopherol, Beta-Carotene Cancer Prevention Study Group (1994) The effect of vitamin E and beta carotene on the incidence of lung cancer and other cancers in male smok­ers. New Engl J. Med. 330, 1029-1035.
  • 10. Kleiner, K. (1996) Vitamin pill fails to fend off cancer. New Scientist January 27, 4.
  • 11. Collins, A.R, Ma, A. & Duthie, S.J. (1995) The kinetics of repair of oxidative DNA damage (strand breaks and oxidised pyrimidines) in human cells. Mutation Res. 336, 69-77.
  • 12. Singh, N.P., McCoy, M.T., Tice, R.R. & Schnei­der, E.L. (1988) A simple technique for quanti­tation of low levels of DNA damage in individ­ual cells. Exp. Cell Res. 175, 184-191.
  • 13. Gedik, C.M, Ewen, S.W.B. & Collins, A.R. (1992) Single-cell gel electrophoresis applied to the analysis of UV-C damage and its repair in human cells. Int J. Radiat. Biol 62, 313-320.
  • 14. Collins, A.R., Duthie, S.J. & Dobson, V.L. (1993) Direct enzymic detection of endoge­nous oxidative base damage in human lympho­cyte DNA. Carcinogenesis 14, 1733-1735.
  • 15. Collins, A.R., DuSinska, M., Gedik, C.M. & StStina, R. (1996) Oxidative damage to DNA: Do we have a reliable biomarker? Envir. Health Persp. 104 (Suppl. 3), 465-469.
  • 16. Hart, D J. & Scott, K.J. (1995) Development and evaluation of an HPLC method for the analysis of carotenoids in food, and the meas­urement of the carotenoid content of vegeta­bles and fruits commonly consumed in the UK. Food Chemistry 54, 101-111.
  • 17. Collins, A.R., Duthie, S.J., Fillion, L., Gedik, C.M., Vaughan, N. & Wood, S.G. (1997) Oxida­tive DNA damage in human cells: The influ­ence of antioxidants and DNA repair. Bio- chem. Soc. Trans. 25, 326-331.
  • 18. Jamieson, D., Chance, B., Cadenas, E. & Boveris, A. (1986) The relation of free radical production to hyperoxia. Annu. Rev. Physiol. 48, 703-719.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-article-3a12bbe5-e421-4e74-8cf7-9bf5a5995027
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