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2005 | 57 | 4 | 355-358
Tytuł artykułu

Cytometryczna analiza indukcji agregacji szczepow Enterococcus faecalis aph2'[plus] przez szczepy aph2'[minus] o roznej czestosci nabywania genu aph2

Warianty tytułu
EN
Flow cytometric analysis of Enterococcus faecalis aph2'[plus] response to aph2'[minus] strains with high and low gene transfer rate
Języki publikacji
PL
Abstrakty
PL
Przy użyciu cytometru obserwowano zmianę stopnia agregacji szczepów Enterococcus faecalis aph2"(+) pod wpływem szczepów aph2"(-), odpowiednio o niskiej i wysokiej częstości nabywania tego genu oraz odmienne efekty oddziaływań pomiędzy szczepami aph2"(+) i aph2"(-)
EN
Resistance genes, as aph2" are usually encoded on conjugate plasmids and spread with high rate among Gram-positive cocci. The conjugation is inducted by recipient strains by secreting specific pheromone involved in formation of mating aggregates with donor cells .The project aimed to check if strain with lower rate of gene transfer differ also from strains with high gene transfer in ability to aggregate to donor strains. In our study we used two aph2"(+), three aph2"(-) with low transfer e and three aph2"(-) with high transfer strains. Each time one aph2"(+) and one aph2" strains were cultivated for 18h in BHI. The bacteria was washed, stained with carboksyfluorescein, and analyzed by flow cytometry in FACS BD cytometr. Relative fluorescence and size of aggregation was used to compare influence on particular stains. In result of induction of aph2"(+) strains with aph2" recipients with high transfer rate we observed increase of size and number aggregates. Surprisingly, induction with aph2" recipients with low transfer rate result in two different reaction of aph2"(+) donors. In case of one of the , according to expectation we do not observe increase of aggregation. Second of the donors aggregate with induction with aph2" recipients with low transfer rate, but in contrast to reaction to presence of other recipients, fluorescence of such aggregates increased. The results show that strain with lower rate of gene transfer in fact differ from strains with high gene transfer in ability to aggregate to donor strains ant that analysis of aggregation alone is insufficient to distinguish between recipients of high and low transfer rate.
Wydawca
-
Rocznik
Tom
57
Numer
4
Strony
355-358
Opis fizyczny
s.355-358,tab.,bibliogr.
Twórcy
  • Akademia Medyczna w Gdansku, ul.Do Studzienki 38, 80-227 Gdansk
autor
autor
Bibliografia
  • 1. Clewell D, Flannagan S, Antiporta S, Danny G. Enterococcal sex precursors are part of of signal sequences of surface lipoproteins. Mol Microbiol 2000; 35: 246-7.
  • 2. Davey H. Flow cytometric techniques for detection of microorganism. Meth Cell Sci 2000; 24: 91- 7.
  • 3. Eaton T, Gasson M. Molecular screening of Enterococcus virulence determinants and potential for genetic exchange between food and medical isolates. Appl Environ Microbiol 2001; 38: 1628-35.
  • 4. Fujimoto S, Clewell D. Regulation of the pADl sex pheromone response of Enterococcus faecalis by direct interaction between the cADl peptide mating signal and the negatively regulating, DNA- binding TraA protein. Proc Natl Acad Sci 1998; 95: 6430-5.
  • 5. Hirt Schlievert P, Dunny G. In vitro induction of virulence and antibiotic resistance transfer in , enterococcus faecalis mediated by sex pheromone sensing system of pCF10. Infect Immun 2002; 70: 716-23.
  • 6. Jarzembowski T. Transfer genów oporności na gentamycynę pomiędzy enterokokami i gronkowcami w warunkach in vitro. Med Dośw Mikrobiol 2002; 54: 177-81.
  • 7. Ma X, Kudo M, Takahashi A, Tanimoto K, i inni. Evidence of nosocomial infection in Japan caused by high-level gentamicin-resistant Enterococcus faecalis and identification of the pheromone-responsive conjugative plasmid encoding gentamicin resistance. J Clin Microbiol 1998; 36: 2460-4.
  • 8. Shiojima M, Tomita H, Tanimoto K i inni. High-level plasmid-mediated gentamicin resistance and pheromone response of plasmids present in clinical isolates of Enterococcus faecalis Antimicrob Agents Chemother 1997; 41: 702-5.
  • 9. Waters C, Dunny M Analysis of functional domains of the Enterococcus faecalis pheromone-induced surface protein aggregation substance . J Bacteriol 2001; 183: 5659-67.
  • 10. Weaver K, Clewell D. Regulation of the pADl sex pheromone response in Enterococcus faecalis: effects of host strain and traA, traB, and C region mutants on expression of an E region pheromone- inducible lacZ fusion. J Bacteriol 1990; 172: 2633-41.
  • 11. Wright R. The sex pheromone system of Enterococcus faecalis: More than just a plasmid collection mechanism? Eur J Biochem 1994; 222: 235-46.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-article-38e30fc5-ebff-4df0-a4df-57952a614474
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