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2001 | 47 | 3 | 497-503
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Opracowanie zestawu diagnostycznego do wykrywania przeciwcial anty-Hypoderma bovis u bydla testem ELISA. 1.Uzyskanie komponentow

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PL
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EN
The aim of these investigations was to prepare the antigen and control sera for the ELISA. Positive control sera were obtained from 163 cows showing clinical symptoms of H. bovis infection and negative control sera from 60 cows free from the infection.The antigen for theELISA was obtained from H. bovis L1 larvae. Three basie fraction were isolated from whole excreted/secreted antigen by elution from gel and by preparative isoelectrofocusing.Using the Western-Blotting method it was demonstrated that Hy A (22-24 kDa and pI 4-5) had the best antigenic property. As can be seen from these investigations the developed ELISA kit for the detection of H. bovis antibodies in cows is highly specific and sensitive.
Wydawca
-
Rocznik
Tom
47
Numer
3
Strony
497-503
Opis fizyczny
s.497-503,rys.,tab.,bibliogr.
Twórcy
autor
  • Panstwowy Instytut Weterynaryjny, ul.Partyzantow 57, 24-100 Pulawy
autor
Bibliografia
  • BARON R. W. 1987. Inhibition of the bovine inflammatory response by warble grub infestations. Research Highlights 1985-86, Lethbridge Research Station, Alberta: 42-44.
  • BOULARD C., GARRONE R. 1978. Characterization of a collagenolytic enzyme from larvae of Hypoderma lineatum (Insecta: Diptera, Oestriform). Camp. Biochem. Physiol. B. 59(3): 251-255.
  • BOULARD C., PETITHORY J. 1977. Serological diagnosis of human hypodermosis: a preliminary report. Vet. Parasitol. 3: 259-263.
  • BOULARD C., SORIA J., SORIA C. 1970. Possibilite d'emploi de la reaction d'hemagglutination passive pour le diagnostic de l'hypodermose, en utilisant comme antigene une collagenasc brute extraite des larves de ler stade d'Hypoderma lineatum. C.R. Acad. Sc. Paris 270 serie D: 1965-1968.
  • CENCEK T., ZIOMKO I. 1998. Peculiarities in the life cycle of warbles and their effect on the control of this disease. Wiad. Parazytol. 44 (3 ): 432.
  • CHABAUDIE N., BOULARD C., HERNANDEZ S., GASCA A., MARTINEZ J., PITHAN K. 1992. Immunomodulation of the bovine defence system by the larval secretions of Hypoderma bovis and H. lineatum. Improvcments in Control Methods for Warble Fly in Cattle and Goats: Workshops Held in Cordoba 8 to 10 May 1991: 115-131.
  • LECROISEY A., TONG N. T., KEIL B. 1983. Hypodermin B, a trypsin-related enzyme from the insect Hypoderma lineatum. Comparison with Hypodermin A and Hypoderma collagenase, two serine proteinases from the same source. Eur. J. Biochem. 134 (2): 261-267.
  • NICOLAS GAULARD I., MOIRE N, BOULARD C. 1995. Effect of the parasite enzyme, hypodermin A, on bovine lymphocyte proliferation and interleukin-2 production via the prostaglandin pathway. Immunology 85(1): 160-165.
  • TONG N. T., IMHOFF J. M., LECROISEY A, KEIL B. 1981. Hypodermin A, a trypsin-like neutral proteinase from the insect Hypoderma lineatum. Biochimica et Biophysica Acta 658(2): 209-219.
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Bibliografia
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