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1998 | 45 | 1 |
Tytuł artykułu

Is the glutathione conjugate of trans-4-hydroxy-2-nonenal transported by the multispecific organic anion transporting-ATPase of human erythrocytes?

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Trans-4-hydroxy-2-nonenal (4-HNE), a cytotoxic end product of lipid peroxidation, is present in normal human blood plasma at concentrations of 0.1-1.0 μM. It can be, however, further metabolized within a cell, and one of the main products is 4-HNE glutathione conjugate (HNE-SG). In human erythrocyte membrane the system for active extrusion of glutathione (GSH) conjugates of various endo- and xenobiotics has been described; it exhibits either a low (Km at submillimolar concentration range) or a high (Km at low micromolar range) affinity for the transported substrates, such as for example S-(2,4-dinitrophenyl)glutathione (Dnp-SG). In the present study it has been shown that the high affinity transport system for Dnp-SG is competitively inhibited by HNE-SG with Ki of 0.2 μM, while 4-HNE inhibits non-competitively the activity of the transport system for Dnp-SG with Ki of 220 μM. These observations point to the possibility that HNE-SG shares the same transport system with GSH conjugates of other endo- and xenobiotics in erythrocytes. This may be of importance for overall detoxification of the organism under oxidative stress.
Wydawca
-
Rocznik
Tom
45
Numer
1
Opis fizyczny
p.59-65,fig.
Twórcy
autor
  • M.Nencki Institute of Experimental Biology, L.Pasteura 3, 02-093 Warsaw, Poland
autor
autor
Bibliografia
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  • 2. Fukuda, A., Osawa, T., Hitomi, K. & Uchida, K. (1996) 4-Hydroxy-2-nonenal cytotoxicity in renal proximal tubular cells: Protein modifica­tion and redox alteration. Arch. Biochenu Bio- phys. 333, 419-426.
  • 3. Pikula, S., Bandorowicz-Pikula, J., Awasthi, S. & Awasthi, Y.C. (1996) ATP-driven trans­port of glutathione S-conjugates, antitumor drugs and xenobiotics from human erythro­cytes. Biochem. Arch. 12, 261-271.
  • 4. Kinter, M. & Roberts, R.J. (1996) Glutathione consumption and glutathione peroxidase inac- tivation in fibroblast cell lines by 4-hydroxy-2-nonenal. Free Radicals Biol. Med. 21, 457-462.
  • 5. Cohn. J.A., Tsai, L., Friguet, B. & Szweda, L.I. (1996) Chemical characterization of a protein- 4-hydroxy-2-nonenal cross-link: Immunoche­mical detection in mitochondria exposed to oxidative stress. Arch. Biochem. Biophys. 328, 158-164.
  • 6. Ullrich, O., Gruñe, T., Henke, W., Esterbauer. H. & Siems, W.G. (1994) Identification of metabolic pathways of the lipid peroxidation product 4-hydroxynonenal by mitochondria isolated from rat kidney cortex. FEBS Lett. 352, 84-86.
  • 7. Ullrich, O., Huser, H., Ehrlich. W. & Gruñe, T. (1997) Intracellular metabolism of 4-hydroxy­nonenal in primary cultures of rabbit synovial fibroblasts. Free Radicals Biol Med. 22, 1153-1157.
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  • 9. Oude Elferink, R.P.J., Meijer, D.K.F., Kuipers, F.,Jansen, P.L.M., Groen, A.K. & Groothius, F. M.M. (1995) Hepatobiliary secretion of or­ganic compounds; Molecular mechanisms of membrane transport. Biochim, Biophys. Acta 1241, 215-268.
  • 10. Paulusma, C.C.. Bosma, P.J., Zaman. G.J.R., Bakker, C.T.M., Otter, M., Scheffer, G.L., Scheper, R.J., Borst, P. & Oude Elferink, R.P.J. (1996) Congenital jaundice in rats with mutation in a multidrug resistance-associated protein gene. Science 271, 1126-1128.
  • 11. LaBelle, E.F., Singh, S.V., Srivastava, S.K. & Awasthi, Y.C. (1986) Dinitrophenyl glu­tathione efflux from human erythrocytes is primary active ATP-dependent transport. Bio­chem. J. 238, 443-449.
  • 12. Pulaski, L., Jedlitschky, G., Leier, L, Buchholz, U. & Keppler, D. (1996) Identification of the multidrug-resistance protein (MRP) as the glutathione-S-conjugate export pump in eryth­rocytes. Eur. J. Biochem. 241, 644-648.
  • 13. Steck, T.L. & Kant, J.A. (1974) Preparation of impermeable ghosts and inside-out vesicles from human erythrocytes membranes. Me­thods Enzymol. 31A, 172-180.
  • 14. Awasthi, Y.C., Garg, H.S., Dao, D.D., Par­tridge, C.A. & Srivastava, S.K. (1981) Enzy­matic conjugation of erythrocyte glutathione with l-chloro-2,4-dinitrobenzene: The fate of glutathione conjugate in erythrocytes and the effect of glutathione depletion on hemoglobin. Blood 58, 733-738.
  • 15. Saxena, M. & Henderson, G.B. (1997) Multiple routes and regulation by tyrosine phosphory­lation characterize the ATP-dependent trans­port of 2,4-dinitrophenyl S-glutathione in inside-out vesicles from human erythrocytes. Arch. Biochem. Biophys. 338, 173-182.
  • 16. Bradford, M.M. (1976) A rapid and sensitive method for quantitation of micro-gram quanti­ties of protein utilizingthe principle of protein dye binding. Anal Biochem. 72, 248-254.
  • 17. Rice-Evans, C.A., Diplock, A.T. & Symons, M.C.R. (1991) Techniques in Free Radical Re­search. Elsevier, Amsterdam.
  • 18. Bligh, E.G. & Dyer, W.J. (1959) A rapid method of total lipid extraction and purifica­tion. Can. J. Biochem. 37, 911-917.
  • 19. Touchstone, J.C., Chen, J.C. & Beaver. K.M. (1979) Improved separation of phospholipids in thin layer chromatography. Lipids 15, 61-62.
  • 20. Rouser, G., Fleischer, S. & Yamamoto, A. (1970) Two dimensional thin layer chroma­tographic separation of polar lipids and deter­mination of phospholipids by phosphorus analysis of spots. Lipids 5, 494-496.
  • 21. Bartosz, G., Sies, H. & Akerboom, T.P.M. (1993) Organic anions exhibit distinct inhibition patterns on the low-Xm and high-tfm transport of <S-(2,4-dinitrophenyl)glutathione through the human erythrocyte membrane. Biochem.. J. 292. 171-174.
  • 22. Akerboom, T.P.M., Bartosz, G. & Sies, H. (1992) Low- and high-Km transport of dinitro- phenyl glutathione in inside out vesicles from human erythrocytes. Biochim. Biophys. Acta 1103, 115-119.
  • 23. Grune, T., Siems, W.G., Kowalewski, J., Zoll- ner, H. & Esterbauer, H. (1991) Identification of metabolic pathways of the lipid peroxida­tion product 4-hydroxynonenal by enterocytes of rat small intestine. Biochem. Int. 25, 963-971.
  • 24. Siems, W.G., Hapner, S.J. & van Kuijk, J.G.M. (1996) 4-Hydroxynonenal inhibits Na*-K*-A- TPase. Free Radicals Biol Med 20,215-223.
  • 25. Chen, J.J., Bertrand, H. & Yu, B.P. (1995) In­hibition of adenine nucleotide translocator by lipid peroxidation products. Free Radicals Biol Med. 19, 583-590.
  • 26. Tsai, L. & Sokoloski, E.A (1995) The reaction of 4-hydroxy-2-nonenal with TV-acetyl-l.-histi- dine. Free Radicals Biol Med. 19, 39-44.
  • 27. Chen, J.J. & Yu, B.P. (1994) Alterations in mi­tochondrial membrane fluidity by lipid peroxi­dation products. Free Radicals Biol Med. 17, 411-418.
Typ dokumentu
Bibliografia
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Identyfikator YADDA
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