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1993 | 40 | 2 |
Tytuł artykułu

Iodine induced alteration in immunological and biochemical properties of thyroglobulin

Autorzy
Warianty tytułu
Języki publikacji
EN
Abstrakty
The influence of iodine-iodide solution on (he biochemical and immunological properties of human thyroglobulin (hTg) were studied. Human Tg preincubated with the iodine-iodide solution is split to small molecular mass fragments after disulphide bridge reduction with dithiothreitol. The peptide bond cleavage by iodine pretreatment and reduction is possibly linked with the coupling reaction of diiodotyrosyl residues. Pretreatment of hTg with iodine-iodide solution at 1 -10 |iM decreased the binding of autoantibodies to hTg. The iodine-iodide induced inactivation of hTg autoepitopes is pH dependent and is possibly caused by iodination of tyrosyl residues present in the epitope structure.
Wydawca
-
Rocznik
Tom
40
Numer
2
Opis fizyczny
p.237-240,fig.
Twórcy
autor
  • Medical Centre of Postgraduate Education, ul.Marymoncka 99, 01-813 Warszawa, Poland
autor
Bibliografia
  • 1. Lissitzky, S. (1984) Thyroglobulin entering into molecular biology. J. Endocrinol. Invest. 7,65- 76.
  • 2. Malthiery, Y., Marriq, C., Berge-Lefranc, J., Henry, M., Lejeune, P., Ruf, J. & Lissitzky, S. (1989) Thyroglobulin structure and function: recent advances. Biochimie 71,195 - 210.
  • 3. Virion, A., Pommier, J. & Nunez, J. (1979) Dissociation of thyroglobulin iodination and hormone synthesis catalyzed by peroxidases. Eur.}. Biochem. 102,549 - 554.
  • 4. Marriq, C., Lejeune, P.J., Venot, N. & Vinet, L. (1989) Hormone synthesis in human thyroglobulin: possible cleavage of the polypeptide chain at the tyrosine donor site. FEBS Lett. 242,414 - 418.
  • 5. Dunn, J.T., Dunn, A.D., Heppner, D.G. & Kim, P.S. (1981) A discrete thyroxine rich iodopeptide of 20 Kdaltons from rabbit thyroglobulin. J. Biol. Chem. 256,942 - 947.
  • 6. Turen, C.D., Chemoff, S.B. & Taurog, A. (1983) Difference on iodinated peptides and thyroid hormone formation after chemical and thyroid peroxidase iodination of human thyroglobulin. Arch. Biochem. Biophys. 222,245 - 258.
  • 7. Virion, A., Deme, D., Pommier, J. & Nunez, J. (1981) The role of iodine and free diiodotyrosine in enzymatic and non-enzymatic hormone synthesis. Eur. ]. Biochem. 118,239 - 245.
  • 8. Derrien, Y. & Roche, M.R. (1948) Recherches sur la preparation et les propriétés de la thyroglobulin pure. Biochem. Biophys. Acta 2,454 -460.
  • 9. Gardas, A. (1991) The influence of iodine on the immunological properties of thyroglobulin and its immunological complexes. Autoimmunity 9, 331-336.
  • 10. O'Sullivan, M.J. & Marks, V. (1981) Methods for the preparation of enzyme-antibody conjugates for use in enzyme immunoassay. Meth. Enzymol. 73,147-166.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-article-25736023-e02f-4ed8-b7cb-ad369f0d6fd0
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