Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2013 | 62 | 1 |
Tytuł artykułu

New PCR test for detection on Candida glabrata based on the molecular target chosen by RAPD technique

Warianty tytułu
Języki publikacji
Rapid, reliable diagnosis is a necessary condition for the successful treatment of infections. Such diagnostic assays are continually being developed. The paper presents a method for selecting the molecular target for PCR-based diagnostics based on the comparison of RAPD patterns. A sequence encoding Candida glabrata CBS138 hypothetical protein was selected. The limit of detection for PCR and real-time PCR reactions with DNA extracted from blood samples spiked with Candida glabrata was estimated at 1 CFU/ml. The application of the assays developed in this study would thus seem to be promising as a complementary method in the diagnostics of C. glabrata infections.
Opis fizyczny
  • Department of Microbiology, Gdansk University of Technology, Narutowicza 11/12, 80-233 Gdansk, Poland
  • Department of Microbiology, Gdansk University of Technology, Narutowicza 11/12, 80-233 Gdansk, Poland
  • Department of Microbiology, Gdansk University of Technology, Narutowicza 11/12, 80-233 Gdansk, Poland
  • Burgener-Kairuz P., J.P. Zuber, P. Jaunin, T.G. Buchman, J. Billie and M. Rossier. 1994. Rapid setection and identification of Candida albicans and Candida glabrata in clinical speciments by species – specific nested PCR amplification of a cytochrome P-450 Lanosterol-Demethylase (L1A1) gene fragment. J. Clin. Microbiol. 32: 1902–1907.
  • Chen Y., J.D. Eisner, M.M. Kattar, S.L. Rassoulian-Barrett, K. Lafe, U. Bui, A.P. Limaye and B.T. Cookson. 2001. Polimorphic internal transcribed spacer region 1 DNA sequences identify medically important yeasts. J. Clin. Microbiol. 39: 4042–4051.
  • Ciardo D.E., G. Schar, E.C. Bottger, M. Altwegg and P.P. Bosshard. 2006. Internal transcribed spacer sequencing versus biochemical profiling for identification of medically important yeasts. J. Clin. Microbiol. 44: 77–84.
  • Fidel P.L., J.A. Vazquez and J.D. Sobel. 1999. Candida glabrata: review of epidemiology, pathogenesis, and clinical disease with comparison to C. albicans. Clin. Microbiol. Rev. 12: 80–96.
  • Fujita S., Y. Seneda, S. Nakaguchi and T. Hashimoto. 2001. Multiplex PCR using internal transcribed spacer 1 and 2 regions for rapid detection and identification of yeast strains. J. Clin. Microbiol. 39: 3617–3622.
  • Jordan J.A. 1994. Identification of four medically important Candida species by using a single primer pair. J. Clin. Microbiol. 32: 2962–2967.
  • Luo G. and T.G. Mitchell. 2002. Rapid identification of pathogenic fungi directly from cultures by using multiplex PCR. J. Clin. Microbiol. 40: 2860–2865.
  • Lyon G.M., S. Karatela, S. Sunay and Y. Adiri. 2010. Antifungal Susceptibility Testing of Candida Isolates from the Candida Surveillance Study. J. Clin. Microbiol. 48: 1270–1275.
  • Mecler I. and U. Nawrot. 2008. Molecular methods of Candida identification. Mikol. Lek. 15: 99–103.
  • Pfaller M.A., D.J. Diekema, S.A. Messer, L. Boyken and R.J. Hollis. 2003. Activities of fluconazole and voriconazole against 1,586 recent clinical isolates of Candida species determined by broth microdilution, disk diffusion, and Etest methods: report from the ARTEMIS Global Antifungal Susceptibility Program 2001. J. Clin. Microbiol. 41: 1440–1446.
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.