PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 36 | 02 |
Tytuł artykułu

Isolation from Proteus mirabilis of a novel class I 5-enopyruvylshikimate-3-phosphate synthase with glyphosate tolerance in transgenic Arabidopsis thaliana

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Applying the genomic library construction process and colony screening, a novel aroA gene encoding 5-enopyruvylshikimate-3-phosphate synthase from Proteus mirabilis was identified and isolated. Furthermore, the transgenic Arabidopsis with the novel aroA gene was obtained to confirm the potential of the novel aroA gene in developing glyphosate-resistant crops.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
36
Numer
02
Opis fizyczny
p.549-554,fig.,ref.
Twórcy
autor
  • Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai, China
autor
  • Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai, China
autor
  • Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai, China
autor
  • Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai, China
autor
  • Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai, China
autor
  • Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai, China
autor
  • Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai, China
autor
  • Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai, China
autor
  • Shanghai Key Laboratory of Agricultural Genetics and Breeding, Biotechnology Research Institute, Shanghai Academy of Agricultural Sciences, 2901 Beidi Road, Shanghai, China
Bibliografia
  • Ausubel FM, Brent RE, Kingston DD, Moore JG, Seidman JAS, Struhl K (1997) Short protocols in molecular biology, 3rd edn. Wiley and Sons, New York
  • Baerson SR, Rodriguez DJ, Tran M, Feng Y, Biest NA, Dill GM (2002) Glyphosate-resistant goosegrass. Identification of a mutation in the target enzyme 5-enolpyruvylshikimate-3-phosphate synthase. Plant Physiol 129(3):1265–1275. doi:10.1104/pp.001560
  • Chen L, Pradhan S, Evans TC Jr (2001) Herbicide resistance from a divided EPSPS protein: the split Synechocystis DnaE intein as an in vivo affinity domain. Gene 263(1–2):39–48 (pii: S0378111900005680)
  • Della-Cioppa G, Bauer SC, Klein BK, Shah DM, Fraley RT, Kishore GM (1986) Translocation of the precursor of 5-enolpyruvylshikimate-3-phosphate synthase into chloroplasts of higher plants in vitro. Proc Natl Acad Sci USA 83(18): 6873–6877
  • Dill GM, Cajacob CA, Padgette SR (2008) Glyphosate-resistant crops: adoption, use and future considerations. Pest Manag Sci 64(4):326–331. doi:10.1002/ps.1501
  • Dun BQ, Wang XJ, Lu W, Zhao ZL, Hou SN, Zhang BM, Li GY, Evans TC Jr, Xu MQ, Lin M (2007) Reconstitution of glyphosate resistance from a split 5-enolpyruvyl shikimate-3-phosphate synthase gene in Escherichia coli and transgenic tobacco. Appl Environ Microbiol 73(24):7997–8000. doi:10.1128/AEM.00956-07
  • Eschenburg S, Healy ML, Priestman MA, Lushington GH, Schonbrunn E (2002) How the mutation glycine96 to alanine confers glyphosate insensitivity to 5-enolpyruvyl shikimate-3-phosphate synthase from Escherichia coli. Planta 216(1):129–135. doi:10.1007/s00425-002-0908-0
  • Funke T, Yang Y, Han H, Healy-Fried M, Olesen S, Becker A, Schonbrunn E (2009) Structural basis of glyphosate resistance resulting from the double mutation Thr97->Ile and Pro101->Ser in 5-enolpyruvylshikimate-3-phosphate synthase from Escherichia coli. J Biol Chem 284(15):9854–9860. doi:10.1074/jbc.M809771200
  • He M, Nie YF, Xu P (2003) A T42M substitution in bacterial 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) generates enzymes with increased resistance to glyphosate. Biosci Biotechnol Biochem 67(6):1405–1409
  • Klee HJ, Muskopf YM, Gasser CS (1987) Cloning of an Arabidopsis thaliana gene encoding 5-enolpyruvylshikimate-3-phosphate synthase: sequence analysis and manipulation to obtain glyphosate-tolerant plants. Mol Gen Genet 210(3):437–442
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Plant Physiol 15(3):473–497
  • Tian YS, Xiong AS, Xu J, Zhao W, Gao F, Fu XY, Xu H, Zheng JL, Peng RH, Yao QH (2010) Isolation from Ochrobactrum anthropi of a novel class II 5-enopyruvylshikimate-3-phosphate synthase with high tolerance to glyphosate. Appl Environ Microbiol 76(17):6001–6005. doi:10.1128/AEM.00770-10
  • Tian YS, Xu J, Xiong AS, Zhao W, Gao F, Fu XY, Peng RH, Yao QH (2012) Functional characterization of Class II 5-enopyruvylshikimate-3-phosphate synthase from Halothermothrix orenii H168 in Escherichia coli and transgenic Arabidopsis. Appl Microbiol Biotechnol 93(1):241–250.doi:10.1007/s00253-011-3443-8
  • Xu J, Tian YS, Peng RH, Xiong AS, Zhu B, Jin XF, Gao F, Fu XY, Hou XL, Yao QH (2010) AtCPK6, a functionally redundant and positive regulator involved in salt/drought stress tolerance in Arabidopsis. Planta 231(6):1251–1260. doi:10.1007/s00425-010-1122-0
  • Zhang X, Henriques R, Lin SS, Niu QW, Chua NH (2006) Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Nat Protoc 1(2):641–646. doi:10.1038/nprot.2006.97
  • Zhou M, Xu H, Wei X, Ye Z, Wei L, Gong W, Wang Y, Zhu Z (2006) Identification of a glyphosate-resistant mutant of rice 5-enolpyruvylshikimate 3-phosphate synthase using a directed evolution strategy. Plant Physiol 140(1):184–195. doi:10.1104/pp.105.068577
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-3e4fdba4-64a8-4f32-b9ae-e9edef2813e8
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.