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2010 | 60 | 2 |
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Study on dynamics of microflora growth in probiotic rennet cheese models

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The production of probiotic cheeses is increasing. Probiotics cheeses always need the application of both traditional mesophilic starter cultures and probiotic strains of lactic acid bacteria (LAB). The manufacture of probiotic cheese only with probiotics is not possible because of their weak growth in milk and the lack of proteolytic activity. The aim of this work was to determine the dynamics of growth of LAB in four probiotic rennet cheese matrices depending on ripening temperature (6°C, 10°C, and 14°C for 8 weeks) and probiotic strains used (Lb. casei Defensis DN- 114001, Bif. animalis subsp. lactis Bb-12, Lb. acidophilus La-5). The present work demonstrates that there are probiotic cultures capable to survive in cheese matrices at a level meeting the therapeutic minimum requirements. The number of lactococci was above 7 log CFU/g, aside from ripening temperature. It was not observed an inhibitory effect of probiotic strain on mesophilic starter culture, independently on the probiotic one used. The growth of Lb. casei strain Defensis DN-114001 was better at 14°C than at 6°C, whereas the population number of Bif. animalis subsp. lactis Bb-12 decreased during the ripening of cheese models, aside from the temperature. Probiotic strain Lb. acidophilus La-5 was characterised by much better viability than Bif. animalis subsp. lactis Bb-12. The reduction of pH value has been observed during the maturation of cheese samples.
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  • Division of Milk Biotechnology, Department of Biotechnology, Microbiology and Food Evaluation, Faculty of Food Sciences, Warsaw University of Life Sciences - SGGW (WULS-SGGW), Nowoursynowska Str. 159c, 02-787 Warsaw, Poland
  • 1. Antunes A.E.C., Grael E.T., Moreno I., Rodrigues L.G., Dourado F.M.; Saccaro D.M., Lerayer A.L.S., Selective enumeration and viability of Bifidobacterium animalis subsp. lactis in a new fermented milk product. Braz. J. Microbiol., 2007, 38, 173–177.
  • 2. Bamforth C.W., Food, Fermentation, and Micro-organisms. 2005, 1st Ed., Blackwell Sciences Publishing, Oxford, pp. 160–168.
  • 3. Boylston T.D., Vinderola C.G., Ghoddusi H.B., Reinheimer J.A., Incorporation of Bifidobacteria into cheeses: challenges and rewards. Int. Dairy J., 2004, 14, 375–387.
  • 4. Daigle A., Roy D., Belanger G., Vuillemard J.C., Production of probiotic cheese (Cheddar-like cheese) using enriched cream fermented by Bifidobacterium infantis. J. Dairy Sci., 1999, 82, 1081–1091.
  • 5. Dinakar P., Mistry V.V., Growth and viability of Bifidobacterium bifidum in Cheddar cheese. J. Dairy Sci., 1994, 77, 2854–2864.
  • 6. Francis F.J., Encyclopaedia of Food Science and Technology. 2000, 2nd Ed., Wiley & Sons, New York, pp. 298–308.
  • 7. Gomes A.M.P., Malcata F.X., Klaver F.A.M., Grande H.J., Incorporation and survival of Bifidobacterium sp. and Lactobacillus acidophilus strain in a cheese product. Neth. Milk Dairy J., 1995, 49, 71–95.
  • 8. Gomes A.M.P., Vieira M.M., Malcata F.X., Survival of probiotic microbial strains in a cheese matrix during ripening: Simulation of rates of salt diffusion and microorganism survival. J. Food Eng., 1998, 36, 281–301.
  • 9. Grattepanche F., Miescher-Schwennninger S., Meile L., Lacroix C., Recent developments in cheese cultures with protective and probiotic functionalities. Dairy Sci. Technol., 2008, 88, 421–444.
  • 10. Hui Y.H., Dairy Science and Technology Handbook. 1993, Vol 2, 1st Ed., Wiley-VCH, New York, pp. 197–236, 359–366.
  • 11. Kasimoglu A., Göncüozlu M., Akgün S., Probiotic white cheese with Lactobacillus acidophilus. Int. Dairy J., 2004, 14, 1067–1073.
  • 12. Mc Brearty S., Ross R.P., Fitzgerald G.F., Collins J.K., Wallace J.M., Stanton C., Influence of two commercially available bifidobacteria cultures on Cheddar cheese quality. Int. Dairy J., 2001, 11, 599–610.
  • 13. Ong L., Henriksson A., Shah N.P., Development of probiotic cheddar cheese containing Lactobacillus acidophilus, Lb. casei, Lb. paracasei and Bifidobacterium spp. and the influence of these bacteria on proteolytic patterns and production of organic acid. Int. Dairy J., 2006, 16, 446–456.
  • 14. Ong L., Shah N.P., Influence of probiotic Lactobacillus acidophilus and L. helveticus on proteolysis, organic acid profiles, and ACE-inhibitory activity of Cheddar cheeses ripened at 4, 8, and 12 degrees C. J. Food Sci., 2008, 73, 111–120.
  • 15. Phillips M., Kailasaphathy K., Tran L., Viability of commercial probiotic cultures (Lb. acidophilus, Bifidobacterium sp., Lb. casei, Lb. paracasei, and Lb. rhamnosus) in Cheddar cheese. Int. J. Food Microbiol., 2006, 108, 276–280.
  • 16. Ross R.P., Fitzgerald G., Collins K., Stanton C., Cheese delivering biocultures: Probiotic cheese. Austr. J. Dairy Technol., 2002, 57, 71–78.
  • 17. Shah N.P., Probiotic bacteria: Selective enumeration and survival in dairy foods. J. Dairy Sci., 2000, 83, 894–907.
  • 18. Stanton C., Gardiner G., Lynch P.B., Collins J.K., Fitzgerald G., Ross R.P., Probiotic cheese. Int. Dairy J., 1998, 8, 491–496.
  • 19. Tharmaraj N., Shah N.P., Selective enumeration of Lactobacillus delbrueckii ssp. bulgaricus, Streptococcus thermophilus, Lactobacillus acidophilus, Bifidobacteria, Lactobacillus casei, Lactobacillus rhamnosus, and Propionibacteria J. Dairy Sci., 2003, 86, 2288–2296.
  • 20. Vinderola C.G., Mocchiutti P., Reinheimer J.A., Interactions among lactic acid starter and probiotic bacteria used for fermented dairy products. J. Dairy Sci., 2002, 85, 721–729.
  • 21. Vinderola C.G., Prosello W., Ghiberto D., Reinheimer J.A., Viability of probiotic (Bifidobacterium, Lactobacillus acidophilus and Lactobacillus casei) and nonprobiotic microflora in Argentinian Fresco cheese. J. Dairy Sci., 2000, 83, 1905–1911.
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