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2016 | 58 | 2 |
Tytuł artykułu

Chemical composition and UVA-protecting activity of extracts from Ligustrum vulgare and Olea europaea Leaves

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Plants belonging to the family Oleaceae have been traditionally used in treatment of various inflammatory skin disorders. One of the most well-known species is Olea europaea L. (olive), cultivated in the Mediterranean countries. Another species is Ligustrum vulgare L. (common privet), occurring particularly in Northern Europe and Asia. The aim of the study was a comparison of the composition of aqueous and ethanolic extracts from leaves of O. europaea and L. vulgare (HPLC-DAD-MS), and determination of the total content of phenolics and flavonoids, as well as the content of the major compound, oleuropein. Secondly, we aimed to study the protective effect of extracts on reactive oxygen species (ROS) production by human fibroblast cells (NHDF), cell viability (MTT assay), and apoptosis rate (Annexin V/propidium iodide staining) after UVA-irradiation. The phytochemical analysis allowed us to identify compounds belonging to the groups of flavonoids, phenylpropanoids and secoiridoids in the extracts. The compounds from the group of lignans (olivil) were identified as being unique to O. europaea extracts. Echinacoside, ligustroflavone and ligustaloside A were identified in L. vulgare extracts in contrast to olive preparations. It was established that the aqueous and ethanolic extracts from leaves of both species, except the privet aqueous extract at a concentration of 5 μg/ml, did not show any significant inhibition of ROS production after UVA-irradiation in the model of NHDF cell line. The aqueous extracts of both species at concentrations of 5 and 25 μg/ml had a protective effect on the viability of UVA-treated cells in contrast to the ethanolic extract. In conclusion, no significant difference in the activity of olive and privet leaf extracts has been observed, which suggests that both plant materials’ extracts, particularly aqueous ones, are effective herbal medicines and photoprotectors, which – to some extent – confirms the use of their preparations in skin disorders.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
58
Numer
2
Opis fizyczny
p.45-55,fig.,ref.
Twórcy
  • Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland
autor
  • Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland
autor
  • Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland
autor
  • Department of Pharmacognosy and Molecular Basis of Phytotherapy, Medical University of Warsaw, Banacha 1, 02-097 Warsaw, Poland
Bibliografia
  • EUROPEAN PHARMACOPOEIA 8.0. 2014. 1-1456. https://www.edqm.eu/en/european-pharmacopoeia-8th-edition-1563.html [13 April 2016].
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  • CVETKOVIĆ D, MARKOVIĆ D, CVETKOVIĆ D, and RADOVANOVIĆ B. 2011. Effects of continuous UV-irradiation on the antioxidant activities of quercetin and rutin in solution in the presence of lecithin as the protective target. Journal of the Serbian Chemical Society 76: 973–985.
  • CZERWIŃSKA ME, GRANICA S, and KISS AK. 2013. Effects of an aqueous extract from leaves of Ligustrum vulgare on mediators of inflammation in a human neutrophils model. Planta Medica 79: 924–932.
  • CZERWIŃSKA ME, ZIAREK M, BAZYLKO A, OSIŃSKA E, and KISS AK. 2015. Quantitative determination of secoiridoids and phenylpropanoids in different extracts of Ligustrum vulgare L. leaves by a validated HPTLC-photodensitometry method. Phytochemical Analysis 26: 253–260.
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  • JASZEWSKA E, SOIN M, FILIPEK A, and NARUSZEWICZ M. 2013. UVA-induced ROS generation inhibition by Oenothera paradoxa defatted seeds extract and subsequent cell death in human dermal fibroblasts. Journal of Photochemistry and Photobiology B: Biology 126: 42–46.
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  • KIMURA Y, and SUMIYOSHI M. 2009. Olive leaf extract and its main component oleuropein prevent chronic ultraviolet B radiation-induced skin damage and carcinogenesis in hairless mice. Journal of Nutrition 139: 2079–2086.
  • KOCA U, SÜNTAR I, AKKOL EK, YILMAZER D, and ALPER M. 2011. Wound repair potential of Olea europaea L. leaf extracts revealed by in vivo experimental models and comparative evaluation of the extracts’ antioxidant activity. Journal of Medicinal Food 14: 140–146.
  • MACKOVÁ A, MUČAJI P, WIDOWITZ U, and BAUER R. 2013. In vitro anti-inflammatory activity of Ligustrum vulgare extracts and their analytical characterization. Natural Product Communications 8: 1509–1512.
  • ONOUE S, SETO Y, OCHI M, INOUE R, ITO H, HATANO T, and YAMADA S. 2011. In vitro photochemical and phototoxicological characterization of major constituents in St. John’s Wort (Hypericum perforatum) extracts. Phytochemistry 72: 1814–1820.
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  • ROMANI A, PINELLI P, MULINACCI N, VINCIERI FF, GRAVANO E, and TATTINI M. 2000. HPLC analysis of flavonoids and secoiridoids in leaves of Ligustrum vulgare L. (Oleaceae). Journal of Agricultural and Food Chemistry 48: 4091–4096.
  • SAVOURNIN C, BAGHDIKIAN B, ELIAS R, DARGOUTH-KESRAOUI F, BOUKEF K, and BALANSARD G. 2001. Rapid high-performance liquid chromatography analysis for the quantitative determination of oleuropein in Olea europaea leaves. Journal of Agricultural and Food Chemistry 49: 618–621.
  • SHARIFI R, PASALAR P, KAMALINEJAD M, DEHPOUR AR, TAVANGAR SM, PAKNEJAD M, MEHRABANI NATANZI M, NOURBAKHSH M, AHMADI ASHTIANI HR, AKBARI M, and RASTEGAR H. 2013. The effect of silymarin (Silybum marianum) on human skin fibroblasts in an in vitro wound healing model. Pharmaceutical Biology 51: 298–303.
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Typ dokumentu
Bibliografia
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Identyfikator YADDA
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