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2011 | 53 | 1 |
Tytuł artykułu

Cytoembryological analysis of causes for poor seed set in alfalfa (Medicago sativa L.)

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EN
Poor seed set is a limiting factor in alfalfa breeding, as it slows the selection response. One strategy used to overcome this problem is to search for mutations of inflorescence morphology. Long-peduncle (lp), branched-raceme (br) and top-flowering (tf) inflorescence mutations increase the number of flowers per inflorescence, but they do not improve seed set per flower. Here we assessed pollen tube growth in styles of those inflorescence mutants and we observed embryo and endosperm development in seeds 1 to 16 days after pollination (DAP). The number of pollen tubes penetrating the style and the ovary was similar in all tested mutants and in the reference cultivar Radius. At 2 DAP, fertilized ovules were 2.7–3.9 times less numerous in certain inflorescence mutants than in the short-raceme cv. Radius. Ovule degeneration progressed at 2–4 DAP in all analyzed plants. Most ovules were not properly developed in the control cultivar (62%), nor in the forms with mutated inflorescence morphology (69–86%). The number of seeds per pod was lowest in the tf form despite its having the highest number of ovules per ovary. It appears that the number of ovules per pistil is not a crucial factor in seed set in alfalfa when fertilization efficiency is very low. Both poor fertilization and gradual ovule degeneration were factors causing poor seed set in the investigated alfalfa genotypes.
Wydawca
-
Rocznik
Tom
53
Numer
1
Opis fizyczny
p.96-101,fig.,ref.
Twórcy
autor
  • Department of General Botany, Adam Mickiewicz University, Umultowska 89, 61-614 Poznan, Poland
autor
autor
Bibliografia
  • BOLANOS-AGUILAR E.-D, HUYGHE C, JULIER B, and ECALLE C. 2000. Genetic variation for seed yield and its components in alfalfa (Medicago sativa L.) populations.Agronomie 20: 333–345.
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  • PEREIRA TNS., ILARSLAN H, and PALMER RG. 1997. Genetic and cytological analyses of three lethal ovule mutants in soybean(Glycine max; Leguminosae). Genome 40:273–285.
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