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2013 | 16 | 1 |
Tytuł artykułu

The porcine brucellosis - evidence of the role of Yersinia enterocolitica O:9 in occurrence of false positive serological reactions

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Języki publikacji
EN
Abstrakty
EN
Forty four pigs with typical characteristics for false positive serological reactions (FPSR) were examined for the presence of Yersinia enterocolitica O:9. The positive reactions were observed in rose bengal test (RBT, N=23 sera), serum agglutination test (SAT, N=16), complement fixation test (CFT, N=9), indirect ELISA (i-ELISA, N=11) in first, and in RBT (N=14), SAT (N=8), CFT (N=7) and i-ELISA (N=18) in second examination, respectively. In bacteriological examination Y. enterocolitica was confirmed in 12 cases. Six of these isolates were identified with PCR as Y. enterocolitica O:9.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
16
Numer
1
Opis fizyczny
p.129-130,ref.
Twórcy
autor
  • Department of Microbiology, National Veterinary Research Institute, Al.Partyzantow 57, 24-100 Pulawy, Poland
autor
  • Department of Microbiology, National Veterinary Research Institute, Al.Partyzantow 57, 24-100 Pulawy, Poland
autor
  • Department of Microbiology, National Veterinary Research Institute, Al.Partyzantow 57, 24-100 Pulawy, Poland
Bibliografia
  • ISO 10273:2003. Microbiology of food and animal feeding stuffs – Horizontal method for the detection of presumptive pathogenic Yersinia enterocolitica.
  • Jungersen G, Sorensen V, Giese SB, Stack A, Riber U (2006) Differentiation between serological responses to Brucella suis and Yersinia enterocolitica serotype O:9 after natural or experimental infection in pigs. Epidemiol Infect 134: 347-357.
  • Lubeck PS, Skurnik M, Ahrens P, Hoorfar J (2003) A Multiplex PCR-detection assay for Yersinia enterocolitica serotype O:9 and Brucella spp. based on the perosamine syntethase gene – Application to Brucella diagnostics. Adv Exp Med Biol 529: 451-453.
  • OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (2012) 7th ed., OIE, Paris.
  • Szulowski K, Iwaniak W, Pilaszek J (2001) Porcine brucellosis in Poland: problems accompanying serological surveys conducted in 1995-2000. Bull Vet Inst Pulawy 45: 153-161.
  • Wannet WJ, Reessink M, Brunings HA, Maas HM (2001) Detection of pathogenic Yersinia enterocolitica by a rapid and sensitive duplex PCR assay. J Clin Microbiol 39: 4483-4486.
  • Weiner M, Iwaniak W, Zlotnicka J, Szulowski K (2010) Diagnosis of bovine brucellosis using traditional serological techniques and fluorescence polarisation assay. Bull Vet Inst Pulawy 54: 485-488.
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Typ dokumentu
Bibliografia
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