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2013 | 22 | 1 |
Tytuł artykułu

Response of epiphytes and endophytes isolated from winter wheat grain to biotechnological and fungicidal treatments

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Języki publikacji
A greenhouse experiment was carried out to determine the effect of biotechnological and fungicide treatments on the colonization of winter wheat kernels by epiphytic and endophytic Pseudomonas and Azotobacter bacteria, filamentous, and yeast-like fungi. Microbiological analyses were performed using two techniques that involved placing kernels on PDA medium, and washing kernels to remove the microbes and plating the wash on selective media. The predominant microbial groups washed from the surface of winter wheat kernels were the pseudomonads and yeast-like fungi. Bacteria of the genus Azotobacter were present primarily inside kernels, and their counts increased after six months of grain storage. The application of Moddus 250 EC and Amistar 250 SC increased the abundance of most microorganisms, including F. poae. The fungicides Corbel 750 EC and Opera Max 147.5 SE, the growth promoter Asahi SL, and the plant resistance inducer Biochicol 020 PC inhibited the growth of endophytic yeast-like and filamentous fungi. Yeast-like fungi formed multicellular clusters on the surface of wheat kernels.
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  • Department of Phytopathology and Entomology, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 17, 10-957 Olsztyn, Poland
  • Department of Phytopathology and Entomology, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 17, 10-957 Olsztyn, Poland
  • Department of Plant Physiology and Biotechnology, University of Warmia and Mazury in Olsztyn, Poland
  • Department of Phytopathology and Entomology, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 17, 10-957 Olsztyn, Poland
  • Department of Phytopathology and Entomology, University of Warmia and Mazury in Olsztyn, Prawocheńskiego 17, 10-957 Olsztyn, Poland
  • 1. EDWARDS S. G. Influence of agricultural practices on Fusarium infection of cereals and subsequent contamination of grain by trichothecene mycotoxins. Toxicol. Lett. 153, 29, 2004.
  • 2. WEGULO S.N., BOCKUS W.W., NOPSA J.H., WOLF E.D., ESKRIDGE K.M., PEIRIS K. H.S., DOWELL F.E. Effects of integrating cultivar resistance and fungicide application on fuzarium head blight and deoxynivalenol in winter wheat. Plant Dis. 95, (5), 554, 2011.
  • 3. DIRECTIVE 2009/128/EC of the European Parliament and of the Council, 2009.
  • 4. LIU E., HASJIM J., DHITAL S., GODWIN I. D., GILBERT R. G. Effect of gibberellins-biosynthesis inhibitor treatment on the physicochemical properties of sorghum starch. J. Cereal Sci. 53, 328, 2011.
  • 5. KHAN M. R, DOOHAN F. Comparison of the efficacy of chitosan with that of a fluorescent pseudomonad for the control Fusarium head blight disease of cereals and associated mycotoxin contamination of grain. Biol. Control 48, 48, 2009.
  • 6. BAUTISTA-BAÑOS S., HERNÁNDEZ-LAUZARDO A.N., VELÁZQUEZ-DEL VALLE, HERNÁNDEZ-LÓPEZ M., AIT BARKA E., BOSQUEZ-MOLINA E., WILSON C.L. Chitozan as a potential natural compound to control pre and postharvest diseases of horticultural commodities. Crop Prot. 25, 108, 2006.
  • 7. GÓRNIK K., GRZESIK M. Effect of Asahi SL on China aster “Aleksandra” seed yield, germination and some metabolic events. Acta Physiol. Plant. 24, (4), 379, 2002.
  • 8. DE FREITAS J. R. Yield and N assimilation of winter wheat (Triticum aestivum L., var. Norstar) inoculated with rhizobacteria. Pedobiologia 44, 97, 2000.
  • 9. NARULA N., KUMAR V., BEHL R.K. Effect of phosphatesolubilizing strains of Azotobacter chroococcum on yield traits and their survival in the rhizosphere of wheat genotypes under field conditions. Acta Agron. Hung. 49, (2), 141, 2001.
  • 10. KUMAR V., BEHL R.K., NARULA N. Establishment of phosphate-solubilizing strains of Azotobacter chroococcum in the rhizosphere and their effect on wheat cultivars under green house conditions. Microbiol Res. 156, 87, 2001.
  • 11. LANDSCHOOT S., AUDENAERT K., WAEGEMAN W., PYCKE B., BEKAERT B., DE BAETS B., HAESAERT G. Connection between primary Fusarium inoculum on gramineous weeds, crop residues and soil samples and the final population on wheat ears in Flanders, Belgium. Crop Prot. 30, 1297, 2011.
  • 12. SCHLÜTER K., KROPF U., KARLOVSKY P. An examination of systemic infections of Fusarium culmorum in Schleswig-Holstein. Gesunde Pflanzen 58, 107, 2006 [In German].
  • 13. STENGLEIN S. A. Fusarium poae: a pathogen that needs more attention. Journal of Plant Pathology 91, (1), 25, 2009.
  • 14. MAGAN N., HOPE R., ALDRED D. Ecophysiology of Fusarium culmorum and mycotoxin production. Advances in Food Mycology. 571, (3), 123, 2006.
  • 15. PATERSON R.R.M., LIMA N. Further mycotoxin effects from climate change. Food Res. Int. 44, 2555, 2011.
  • 16. MANKEVIČIENÉ A., BUTKUTÉ B., GAURILČIKIENE I., DABKEVIČIUS Z. Risk assessment of Fusarium mycotoxins in Lithunian small cereal grains. Food Control 22, 970, 2011.
  • 17. KING E., WARD M. K., RANEY DE. Two simple media for the demonstration of pyocyanin and fluorescein. J. Lab. Clin. Med. 44, 301, 1954.
  • 18. MARTYNIUK S., MARTYNIUK M. Occurence of Azotobacter spp. in Polish soils. Pol. J. Environ. Stud. 12, (3), 371, 2003.
  • 19. MARTIN J.P. Use of acid, rose Bengal and streptomycyn in the plate metod for estimating soil fungi. Soil Sci. 38, 215, 1950.
  • 20. ARX J.A. The genera of fungi sporulating in pure culture. Verlag von J. Cramer. Lehre. pp. 288, 1970.
  • 21. LESLIE J. F., SUMMERRELL B. A., BULLOCK S. The Fusarium laboratory manual. Blackwell Publishing, Oxford, pp. 388, 2006.
  • 22. FASSATIOVÁ O. Microscopic fungi in technical microbiology. Wyd. Naukowo-Techniczne, Warszawa, pp. 256, 1983 [In Polish].
  • 23. LACA A., MOUSIA Z., DIAZ M., WEBB C., PANDIELLA S. Distribution of microbial contamination within cereal grains. J. Food Eng. 72, 332, 2006.
  • 24. BIRCK N. M. M., LORINI I., SCUSSEL V. M. Fungus and mycotoxins in wheat grain at post harvest. In: 9th International Working Conference on Stored Product Protection: 198-205, 2006.
  • 25. PLĄSKOWSKA E., CHRZANOWSKA-DROŻDŻ B. The health status of winter durum wheat grain in relation to fungicide treatment. Phytopathologia 54, 23, 2009.
  • 26. WACHOWSKA U. Activity of fungicides against epiphytic yeast-like fungi of winter wheat. Pol. J. Environ. Stud. 18, (6), 1169, 2009.
  • 27. MARTYNIUK S., OROŃ J., MĄCZKA M. Characterization of fungi occuring on heades of winter wheat cultivated under conventional and ecological systems. Progress in Plan Pretection 49, (3), 1309, 2009 [In Polish].
  • 28. RAULIO M., WILHELMSON A., SALKINOJA-SALONEN M., LAITILA A. Ultrastructure of biofilms formed on barley kernels during malting with and without starter culture. Food Microbiol. 26, 437, 2009.
  • 29. ZHANG Y-J., ZHANG X., CHEN C-J., ZHOU M-G., WANG H-C. Effects of fungicides JS399-19, azoxystrobin, tebuconazole, and carbendazim on the physiological and biochemical indices and grain yield of winter wheat. Pestic. Biochem. Phys. 98, 151, 2010.
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