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2016 | 56 |
Tytuł artykułu

Isolation, identification and extracellular enzymatic activity of culturable extremely halophilic archaea and bacteria of IncheBoroun wetland

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Extremely halophilic diversity of IncheBroun wetland located in the north of Iran was investigated by using culture-dependent methods. Sampling was carried out in May and September 2014. In each sampling 4 distinct regions of wetland were analyzed by using complex media like MGM, JCM168, MH1 and an alkaliphilic medium containing 23% salts. After incubation at 40˚C, a total of 406 isolates and 2.1 × 106 CFU/ml were obtained in culture media. Among them 361 isolates were obtained from MGM and 39 isolates from JCM 168, 3 isolates from MH1 and 3 isolates from the alkaliphilic media. Initial morphological, biochemical and physiological tests were performed. Production of 4 hydrolytic enzymes by 45 selected strains was assayed qualitatively. A total of 38, 19 and 6 strains were able to produce lipase, DNase and amylase activity. Protease activity was not observed among strains. As total 45 strains were selected randomly and phylogenetic analysis of 16S rRNA was performed for them. Among selected strains 40 isolated strians belonged to Haloarchaea and were belonged to the genera: Haloarcula(30%), Halorubrum(27.5%), Haloferax(17.5%), Halobellus (10%), Halogeometricum(5.2%), Halobacterium(2.6%), Halolamina(2.6%), Halorhabdus (2.6%) and Halostagnicola (2.6%). Haloarcula and Halorubrum were the dominant populations. A total of 5 strains belonged to domain of Bacteria and were similar to members of Rhodovibrio (40%), Pseudomonas (40%) and Salicola (20%).
Słowa kluczowe
Wydawca
-
Rocznik
Tom
56
Opis fizyczny
p.40-51,fig.,ref.
Twórcy
autor
  • Microorganisms Bank, Iranian Biological Resource Centre (IBRC), ACECR Tehran, Iran
  • Extremophiles Laboratory, Department of Microbiology, School of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran
autor
  • Microbiology Department, Faculty of Biological Sciences, Islamic Azad University North Tehran Branch, Tehran, Iran
  • Applied Virology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
autor
  • Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Bibliografia
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  • DOI References
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  • [19] L. Maturrano, et al., Microbial diversity in Maras salterns, a hypersaline environment in the Peruvian Andes, Applied and Environmental Microbiology. 72(6) (2006) 3887-3895. 10.1128/aem.02214-05
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  • [31] A.M. Kakhki, M.A. Amoozegar and E.M. Khaledi, Diversity of hydrolytic enzymes in haloarchaeal strains isolated from salt lake, International Journal of Environmental Science & Technology. 8(4) (2011) 705-714. 10.1007/bf03326255
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Typ dokumentu
Bibliografia
Identyfikator YADDA
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