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2013 | 22 | 1 |
Tytuł artykułu

Novel polymorphisms detected in the prolactin receptor gene of Yili horse (Equus caballus) by PCR-SSCP

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The prolactin receptor (PRLR) is a receptor for a polypeptide hormone that stimulates mammary gland development and lactation in mammals. Associations between the PRLR genotype and cow and goat milk traits have been reported. To date, the nucleotide polymorphisms within the PRLR gene in equine species have not been investigated. Single-nucleotide polymorphism (SNP) is the most abundant form of genetic variation and a resource for useful genetic traits. In the present study, SNPs within the coding regions of the PRLR gene were detected by polymerase chain reaction-single-strand conformation polymorphism (SSCP) and DNA sequencing on the Yili horse of China. Ten novel SNPs were identified in the PRLR gene: one located within exon 4 (XM_001500104: c.156 C>T); one within exon 5 (c.312 T>C); and eight within exon 10 (c.1062 T>C, c.1083 G>A, c.1226 A>C, c.1233 C>T, c.1279 C>A, c.1749 A>T, c.1764 G>C, and c.1778 T>A). The mutation allele frequencies and effective allele numbers ranged from 0.10 to 0.30 and from 1.2195 to 1.7240, respectively. Four of the identified SNPs are non-synonymous and involve four amino acid substitutions, namely, p.H409P (c.1226 A>C), p.H427N (c.1279 C>A), p.Q588H (c.1764 G>C), and p.I593K (c.1778 T>A), in the cytoplasmic domain of the PRLR, which plays a fundamental role in signal transduction. Theoretically, the four nucleotide substitutions would result in altered spatial structure and functions of the PRLR molecules. Further study is required, however, to determine whether these mutations affect the milk yield of the Yili horse.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
22
Numer
1
Opis fizyczny
p.70-76,fig.,ref.
Twórcy
autor
  • Xinjiang Key Laboratory of Meat- and Milk-Production, Herbivore Nutrition, College of Animal Science, Xinjiang Agricultural University Urumqi 830052, P. R. China
  • Chemical and Environmental Science Department, Kashgar Teacher’s College Kashgar 844006, P. R. China
autor
  • Xinjiang Key Laboratory of Meat- and Milk-Production, Herbivore Nutrition, College of Animal Science, Xinjiang Agricultural University Urumqi 830052, P. R. China
autor
  • Xinjiang Key Laboratory of Meat- and Milk-Production, Herbivore Nutrition, College of Animal Science, Xinjiang Agricultural University Urumqi 830052, P. R. China
autor
  • Xinjiang Key Laboratory of Meat- and Milk-Production, Herbivore Nutrition, College of Animal Science, Xinjiang Agricultural University Urumqi 830052, P. R. China
Bibliografia
  • Bassam B.J., Caetano-Anolle´s G., Gresshoff P.M., 1991. Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal. Biochem. 196, 80–83
  • Berkowicz E.W., Magee D.A., Sikora K.M., Berry D.P., Howard D.J., Mullen M.P., Evans R.D., Spillane C., MacHugh D.E., 2011. Single nucleotide polymorphisms at the imprinted bovine insulinlike growth factor 2 (IGF2) locus are associated with dairy performance in Irish Holstein-Friesian cattle. J. Dairy Res. 78, 1–8
  • Brym P., Kaminski S., Wojcik E., 2005. Nucleotide sequence polymorphism within exon 4 of the bovine prolactin gene and its associations with milk performance traits. J. Appl. Genet. 45, 179–185
  • Hall T.A., 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids. Symp. Ser. 41, 95-98
  • Han B., Zhang H.L., Zeng L., Yang B., Abula R., Xu X.L. Chen Y., 2011. The Polymorphisms of β2-adrenergic receptor gene on two cattle breeds in China. Asian J. Anim. Vet. Adv. 6, 715–722
  • Lü A., Hu X., Chen H., Jiang J., Zhang C., Xu H., Gao X., 2010. Single nucleotide polymorphisms in bovine PRL gene and their associations with milk production traits in Chinese Holsteins. Mol. Biol. Rep. 37, 547-551
  • Lü A., Hu X., Chen H., Dong Y., Zhang Y., Wang X., 2011. Novel SNPs of the bovine PRLR gene associated with milk production traits. Biochem. Genet. 49, 177-189
  • Maruya E., Saji H., Yokoyama S., 1996. PCR-LIS-SSCP (Low ionic strength single-stranded conformation polymorphism)--a simple method for high-resolution allele typing of HLA-DRB1, -DQB1, and -DPB1. Genome Res. 6, 51–57
  • Mullen M.P., Berry D.P., Howard D.J., Diskin M.G., Lynch C.O., Berkowicz E.W., Magee D.A., MacHugh D.E., Waters S.M., 2010. Associations between novel single nucleotide polymorphisms in the Bos taurus growth hormone gene and performance traits in Holstein-Friesian dairy cattle. J. Dairy Sci. 93, 5959–5969
  • Mullen M.P., Berry D.P., Howard D.J., Diskin M.G., Lynch C.O., Giblin L., Kenny D.A., Magee D.A., Meade K.G., Waters S.M., 2011. Single nucleotide polymorphisms in the insulin-like growth factor 1 (igf-1) gene are associated with performance in HolsteinFriesian dairy cattle. Front. Genet. 2, 3
  • Oto M., Miyake S., Yuasa Y., 1993. Optimization of nonradioisotopic single strand conformation polymorphism analysis with a conventional minislab gel electrophoresis apparatus. Anal. Biochem. 213, 19–22
  • Sambrook J., Russell D.W., 2001. Molecular Cloning:A Laboratory Manual.3rd Edn.,Cold Spring Harbor Laboratory Press, New York, ISBN:978087965774, pp., 1–99
  • Sun R.P, Wang L.X., Zhu G.Q., Wan G.G., Song Y.X., Liang Z.Y., Cao B.Y., 2008. Polymorphism of exon 10 of prolactin receptor gene and its relationship with milk performance of Xinong Saanen dairy goat. Acta Vet. Zootech. Sinica 39, 1654–1660
  • Visiers I., Braunheim B.B., Weinstein H., 2000. Prokink: a protocol for numerical evaluation of helix distortions by proline. Protein Eng. 13, 603–606
  • Yao X.K. 2011. Preliminery Study on chemical-physical indicators of mare`s milk and lactation character of Yili horse, New Kirgiz horse and hybrid horse. Ph.D thesis, Xinjiang Agricultural University (China)
  • Zhang J.L., Zan L.S., Fang P., Zhang F., Shen G. Tian W.Q., 2008. Genetic variation of PRLR geneand association with milk performance traits in dairy cattle. Can. J. Anim. Sci. 88, 33–39
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-06d8045a-f0cd-4a96-916e-bba4ce4eeed1
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