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2015 | 37 | 02 |
Tytuł artykułu

Identification of heat-stable proteins in imbibed Cowpea (Vigna unguiculata) seeds

Autorzy
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Heat-stable protein fraction in seeds is believed to enrich many proteins functioning in the acquisition of stress-tolerance of seeds. In this study, the composition of heat-stable protein fraction in imbibed cowpea (Vigna unguiculata) seeds was analyzed by SDS-PAGE and twodimensional gel electrophoresis coupled with mass spectrometry. The results indicated that approximately 12.4 % of seed soluble proteins were stable after heat treatment at 100 C for 10 min. Twenty-two putative heat-stable proteins were identified using MALDI-TOF/TOF MS. Most of these heat-stable proteins were late embryogenesis abundant proteins, and there were other stress-related proteins including Cu/Zn superoxide dismutase and 17.4 kDa Class I heat-shock protein. A cyclophilin protein, a cleavage and polyadenylation specificity factor and a Pumilio-family RNA binding protein were also present in the heat-stable fraction. The identified heat-stable proteins were more hydrophilic proteins and may accumulate to stabilize cellular components and maintain seed viability during seed development and germination.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
37
Numer
02
Opis fizyczny
Article: 24 [7 p.], fig.,ref.
Twórcy
autor
  • Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, 135 Xin-gang Road, Guangdong 510275, China
autor
  • Guangdong Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-sen University, 135 Xin-gang Road, Guangdong 510275, China
Bibliografia
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Typ dokumentu
Bibliografia
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Identyfikator YADDA
bwmeta1.element.agro-06cc01a6-4078-4e9e-b1d9-36de320f6343
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