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2012 | 33 | 3 | 411-429
Tytuł artykułu

Determination of phase diagrams and thermoseparation behaviour of aqueous two-phase systems composed of ethylene oxide–propylene oxide random copolymer and potassium phosphate

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The application of aqueous two-phase systems (ATPS) is a cost-effective and simple method of protein separation (including enzymes) from complex systems. The first stage of designing the protein purification process in an ATPS involves the identification of the conditions for the formation of a given extraction system. For this purpose, the conditions for the formation of ATPSs in a thermoseparating EO50PO50 polymer/potassium phosphates system have been studied. Factors determining the ATPS formation comprised: separation temperature (4ºC or 20ºC), phosphate solution pH (6, 7.5 or 9) as well as the concentration of NaCl introduced into the systems (0.085 M, 0.475 M and 0.85 M). ATPS without NaCl were prepared as well. The conditions for the formation of the primary EO50PO50/potassium phosphate ATPS were determined with their phase diagrams. It was observed that with an increase of phosphate pH and NaCl concentration in the system, there was a decrease of the EO50PO50 and phosphate concentrations necessary to form a primary ATPS. After the primary two-phase separation, the top phase (rich in the EO50PO50 polymer) was partitioned from the bottom phase (rich in phosphates). Next, by means of polymer phase thermoseparation, a secondary two-phase system was formed. In the secondary EO50PO50/phosphate ATPS, the bottom phase was formed by the concentrated EO50PO50 polymer (30-80% concentration), while the top phase by a solution composed mainly of water, containing phosphate ions and remains of EO50PO50 polymer (3-7%).
Wydawca

Rocznik
Tom
33
Numer
3
Strony
411-429
Opis fizyczny
Daty
wydano
2012-10-01
otrzymano
zaakceptowano
online
2012-10-31
Twórcy
  • Poznan University of Life Sciences, Faculty of Food Science and Nutrition, Department of Biotechnology and Food Microbiology, Wojska Polskiego 48, 60 – 627 Poznań, Poland, rdembcz@up.poznan.pl
  • Poznan University of Life Sciences, Faculty of Food Science and Nutrition, Department of Biotechnology and Food Microbiology, Wojska Polskiego 48, 60 – 627 Poznań, Poland
  • Poznan University of Life Sciences, Faculty of Food Science and Nutrition, Department of Biotechnology and Food Microbiology, Wojska Polskiego 48, 60 – 627 Poznań, Poland
Bibliografia
  • Adler N., 1965. Residual ethylene oxide and ethylene glycol in ethylene oxide sterilized pharmaceuticals. J. Pharm. Sci., 54, 735-742. DOI: 10.1002/jps.2600540516.[Crossref]
  • Albertsson P. A., 1986. Partition of Cell Particles and Macromolecules. Wiley, New York.
  • Albertsson P. A., Tjerneld F., 1994. Phase diagrams, In: Walter H., Johansson G. (Eds.), Methods in enzymology, vol. 228. Academic Press, San Diego, 3-13.
  • Alderton G., Fevold H.L., 1946. Direct crystallization of lysozyme from egg white and some crystalline salts of lysozyme. J. Biol. Chem., 164, 1-5.
  • Bakshi M. S., Sachar S., Yoshimura T., Esumi K., 2004. Association behavior of poly(ethylene oxide)- poly(propylene oxide)-poly(ethylene oxide) block copolymers with cationic surfactants in aqueous solution. J. Colloid Interface Sci., 278, 224-233. DOI: 10.1016/j.jcis.2004.05.025.[Crossref]
  • Banik R. M., Santhiagu A., Kanari B., Sabarinath C. and Upadhyay S. N., 2003. Technological aspects of extractive fermentation using aqueous two-phase systems. World J. Microbiol. Biotechnol., 19, 337-348. DOI: 10.1023/A:1023940809095.[Crossref]
  • Berggren K., Johansson H. O., Tjerneld F., 1995. Effects of salts and the surface hydrophobicity of proteins on partitioning in aqueous two-phase systems containing thermoseparating ethylene oxide-propylene oxide copolymers. J. Chromatogr. A, 718, 67-79. DOI: 10.1016/0021-9673(95)00657-5.[Crossref]
  • Cunha M. T., Cabral J. M. S., Tjerneld F., Aires-Barros M. R., 2000. Effect of salts and surfactants on the partitioning of Fusarium solani pisi cutinase in aqueous two-phase systems of thermoseparating ethylene oxide/propylene oxide random copolymer and hydroxypropyl starch. Bioseparation, 9, 203-209. DOI: 10.1023/A:1008132108117.[Crossref]
  • Cunha M. T., Tjerneld F., Cabral J. M. S, Aires-Barros M. R., 1998. Effect of electrolytes and surfactants on the thermoseparation of an ethylene oxide-propylene oxide random copolymer in aqueous solution. J. Chromatogr. B: Biomed. Sci. Appl., 711, 53-60. DOI: 10.1016/S0378-4347(98)00021-8.[Crossref]
  • Czaczyk K., Cyplik P., Mueller A., 2003. The method of determination of inorganic anions in water by use of HPLC with UV detection. Aparatura Badawcza i Dydaktyczna, 8, 27-32 (in Polish).
  • Dembczyński R., Białas W., Jankowski T., 2010b. Recycling of phase components during lysozyme extraction from hen egg white in the EO50PO50/K2HPO4 aqueous two-phase system. Biochem. Eng. J., 51, 24-31. DOI: 10.1016/j.bej.2010.04.011.[WoS]
  • Dembczyński R., Białas W., Regulski K., Jankowski T., 2010a. Lysozyme extraction from hen egg white in an aqueous two-phase system composed of ethylene oxide-propylene oxide thermoseparating copolymer and potassium phosphate. Process Biochem., 45, 369-374. DOI:10.1016/j.procbio.2009.10.011.[Crossref][WoS]
  • de Oliveira M. C., de Abreu Filho M. A. N., Pessôa Filho P. A., 2007. Phase equilibrium and protein partitioning in aqueous two-phase systems containing ammonium carbamate and block copolymers PEO-PPO-PEO. Biochem. Eng. J., 37, 311-318. DOI: 10.1016/j.bej.2007.05.010.[Crossref][WoS]
  • Dyr J. E. and Suttnar J., 1997. Separation used for purification of recombinant proteins. J. Chromatogr. B: Biomed. Sci. Appl., 699, 383-401. DOI: 10.1016/S0378-4347(97)00201-6.[Crossref]
  • Elversson J., Millqvist-Fureby A., 2005. Aqueous two-phase systems as a formulation concept for spray-dried protein. Int. J. Pharm., 294, 73-87. DOI: 10.1016/j.ijpharm.2005.01.015.[Crossref]
  • Johansson H.-O., Karlstrom G., Tjerneld F., 1997. Temperature-induced phase partitioning of peptides in water solutions of ethylene oxide and propylene oxide random copolymers. Biochim. Biophys. Acta, 1335, 315-325. DOI: 10.1016/S0304-4165(96)00150-X.[Crossref]
  • Kopeć W., Chrzanowska J., Karkoszka K., Lorenc J., Polanowski A., Szałak T., Trziszka T., 2000. A method of obtaining a preparation of lysozyme salt. Bulletin of the Polish Patent Office, 15 (693), 29-30 (in Polish).
  • Kepka C., Collet E.,Roos F., Tjerneld F., Veide A., 2005. Two-step recovery for tryptophan tagged cutinase: Interfacing aqueous two-phase extraction and hydrophobic interaction chromatography. J. Chromatogr. A, 1075, 33-41. DOI: 10.1016/j.chroma.2005.03.054.[Crossref]
  • Leja K., Dembczyński R., Białas W., Jankowski T., 2009. Production of dry Lactobacillus rhamnosus GG preparations by spray drying and lyophilization in aqueous two-phase systems. Acta Sci. Pol., Technol. Aliment., 8, 39-49.
  • Li W., Lin D.-Q., Zhu Z.-Q., 2000. Measurement of water activities and prediction of liquid-liquid equilibria for water + ethylene oxide-propylene oxide random copolymer + ammonium sulfate systems. Fluid Phase Equilib., 175, 7-18. DOI: 10.1016/S0378-3812(00)00452-0.[Crossref]
  • Li W., Zhu Z.-Q., Lin D.-Q., 1999. Liquid−liquid equilibria of aqueous two-phase systems containing ethylene oxide−propylene oxide random copolymer and ammonium sulfate. J. Chem. Eng. Data, 44, 921-925. DOI: 10.1021/je990049o.[Crossref]
  • Lienqueo M.E. and Asenjo J.A., 2000. Use of expert systems for the synthesis of downstream protein processes. Comput. Chem. Engng., 24, 2339-2350. DOI: 10.1016/S0098-1354(00)00590-1.[Crossref]
  • Materna K., Cote G., Szymanowski J., 2004. Cloud point of aqueous solutions containing oxyethylated methyl dodecanoates: effects of surfactant hydrophilicity, nature of added electrolyte and water activity. J. Colloid Interface Sci., 269, 466-471. DOI: 10.1016/j.jcis.2003.08.002.[Crossref]
  • Merchuk J.C., Andrews B. A., Asenjo J.A., 1998. Aqueous two-phase systems for protein separation. Studies on phase inversion. J. Chromatogr. B: Biomed. Sci. Appl., 711, 285-293. DOI: 10.1016/S0378-4347(97)00594-X.[Crossref]
  • Mishima K., Matsuyama K., Ezawa M., Taruta Y., Takarabe S., Nagatani M., 1998. Interfacial tension of aqueous two-phase systems containing poly(ethylene glycol) and dipotassium hydrogenphosphate. J. Chromatogr. B: Biomed. Sci. Appl., 711, 313-318. DOI: 10.1016/S0378-4347(97)00660-9.[Crossref]
  • Mistry S.L., Kaul A., Merchuk J.C., Asenjo J.A., 1996. Mathematical modelling and computer simulation of aqueous two-phase continuous protein extraction. J. Chromatogr. A, 741, 79-84. DOI: 10.1016/0021-9673(96)00179-3.[Crossref]
  • Owen R.O., Chase H.A., 1997. Direct purification of lysozyme using continuous counter-current expanded bed adsorption. J. Chromatogr. A, 757, 41-49. DOI: 10.1016/S0021-9673(96)00680-2.[Crossref]
  • Pereira M., Wu Y.-T., Venancio A., Teixeira J., 2003. Aqueous two-phase extraction using thermoseparating polymer: a new system for the separation of endo-polygalacturonase. Biochem. Eng. J., 15, 131-138. DOI: 10.1016/S1369-703X(02)00190-0.[Crossref]
  • Przybycien T. M., Pujar N. S. and Steele L. M., 2004. Alternative bioseparation operations: life beyond packedbed chromatography. Curr. Opin. Biotech., 15, 469-478. DOI: 10.1016/j.copbio.2004.08.008.[Crossref]
  • Rito-Palomares M., 2004. Practical application of aqueous two-phase partition to process development for the recovery of biological products. J. Chromatogr. B: Biomed. Sci. Appl., 807, 3-11. DOI: 10.1016/j.jchromb.2004.01.008.[Crossref]
  • Rosa P. A. J., Azvedo A. M., Ferreira I. F., Sommerfeld S., Bäcker W., Aires-Barros M. R., 2009. Downstream processing of antibodies: Single-stage versus multi-stage aqueous two-phase extraction. J. Chromatogr. A, 1216, 8741-8749. DOI: 10.1016/j.chroma.2009.02.024.[WoS][Crossref]
  • Salabat A., 2001. The influence of salts on the phase composition in aqueous two-phase systems: experiments and predictions. Fluid Phase Equilib., 187-188, 489-498. DOI: 10.1016/S0378-3812(01)00569-6.[Crossref]
  • Sarubbo L.A., Oliveira L.A., Porto A.L.F., Duarte H.S., Carneiro-Leão A.M.A., Lima-Filho J.L., Campos-Takaki G.M., Tambourgi E.B., 2000. New aqueous two-phase system based on cashew-nut tree gum and poly(ethylene glycol). J. Chromatogr. B: Biomed. Sci. Appl., 743, 79-84. DOI: 10.1016/S0378-4347(99)00516-2.[Crossref]
  • Schügerl K., Hubbuch J., 2005. Integrated bioprocesses. Curr. Opin. Biotech., 8, 294-300. DOI: 10.1016/j.mib.2005.01.002.[Crossref]
  • Sebastiao M. J., Cabral J.M. and Aires-Barros M. R., 1996. Improved purification protocol of a Fusarium solani pisi recombinant cutinase by phase partitioning in aqueous two-phase systems of polyethylene glycol and phosphate. Enzyme Microb. Tech., 18, 251-260. DOI: 10.1016/0141-0229(95)00045-3.[Crossref]
  • Silverio S.C., Rodriguez O., Teixeira J.A., Macedo E.A., 2010. Liquid-liquid equlibria of UCON + (sodium or potassium) phosphate salt aqueous two-phase systems at 23°C. J. Chem. Eng. Data, 55, 1285-1288. DOI: 10.1021/je900628m.[WoS][Crossref]
  • Sinha J., Dey P. K., Panda T., 2000. Aqueous two-phase: the system of choice for extractive fermentation. Appl. Microbiol. Biot., 54, 476-486. DOI: 10.1007/s002530000342.[Crossref]
  • Su C.-K., Chiang B.H., 2006. Partitioning and purification of lysosyme from chicken egg white using aqueous two-phase system. Process Biochem., 41, 257-263. DOI: 10.1016/j.procbio.2005.06.026.[Crossref]
  • Svensson M., Berggren K., Veide A., Tjerneld F., 1999. Aqueous two-phase systems containing self-associating block copolymers. Partitioning of hydrophilic and hydrophobic biomolecules. J. Chromatogr. A, 839, 71-83. DOI: 10.1016/S0021-9673(99)00038-2.[Crossref]
  • Torchilin V.P., 2001. Structure and design of polymeric surfactant-based drug delivery systems. J. Control. Release, 73, 137-172. DOI: 10.1016/0141-0229(95)00045-3.[Crossref]
  • Walter H., Brooks D.B. and Fisher D., 1985. Partitioning in Aqueous Two-Phase Systems: Theory, Methods, Uses, and Applications to Biotechnology. Academic Press, Orlando, FL.
  • Wu Y.-T., Zhu Z.-Q., 1999. Modeling of interfacial tension of aqueous two-phase systems. Chem. Eng. Sci., 54, 433-440. DOI: 10.1016/S0009-2509(98)00255-3.[Crossref]
  • Xu Y., de Souza M. A., Ribeiro-Pontes M., Z., Vitolo M., Pessoa-Jr A., 2001. Liquid-liquid extraction of pharmaceuticals by aqueous two-phase systems. Braz. J. Pharm. Sci., 37, 305-320.
  • Zafarani-Moattar M. T., Hamzehzadeh Sh., 2005. Liquid-liquid equilibria of aqueous two-phase systems containing polyethylene glycol and sodium succinate or sodium formate. Calphad Comput. Coupling Ph. Diagrams Thermochem., 29, 1-6. DOI: 10.1016/j.calphad.2005.01.004.[WoS][Crossref]
  • Zafarani-Moattar M. T., Sadeghi R., 2001. Liquid-liquid equilibria of aqueous two-phase systems containing polyethylene glycol and sodium dihydrogen phosphate or disodium hydrogen phosphate: Experiment and correlation. Fluid Phase Equilib., 181, 95-112. DOI: 10.1016/S0378-3812(01)00373-9.[Crossref]
  • Zafarani-Moattar M. T., Tolouei S., 2008. Liquid-liquid equilibria of aqueous two-phase systems containing polyethylene glycol 4000 and di-potassium tartate, potassium sodium tartrate, or di-potassium oxalate: Experiment and correlation. Calphad Comput. Coupling Ph. Diagrams Thermochem., 32, 655-660. DOI: 10.1016/j.calphad.2008.09.006.[WoS][Crossref]
  • Zaslavsky B. Y., 1995. Aqueous Two-Phase Partitioning - Physical Chemistry and Bioanalytical Applications. Marcel Dekker Inc., New York, 82.
  • Zielińska-Dawidziak M., Jankowski T., 2002a. The separation of biotechnology products in aqueous two-phase systems. Part I - Theoretical background. Biotechnologia, 1 (56), 142-152 (in Polish).
  • Zielińska-Dawidziak M., Jankowski T., 2002b. The separation of biotechnology products in aqueous two-phase systems. Part II - Applications. Biotechnologia, 2 (57), 102-112 (in Polish).
Typ dokumentu
Bibliografia
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Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_v10176-012-0036-8
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