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2010 | 82 | 10 | 547-552
Tytuł artykułu

Influence of Vitamin E Given Intraperitoneally to Prevent Peritoneal Adhesions in Rats

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The aim of the study was to evaluate the influence of vitamin E administered intraperitoneally on the prevention of peritoneal adhesion formation in rats on the basis of macroscopic and microscopic assessment of the adhesions.Material and methods. Experimental studies were performed on 50 Sprague-Dawley male rats, which were randomly divided into 5 groups, 10 rats in a group. Experimental group I (EI) included 10 rats which had peritoneal adhesions provoked by scraping of the wall of cecum and parietal peritoneum followed by intraperitoneal administration of vitamin E in the dose of 100 mg/kg body weight. Experimental group II (EII) included 10 rats which had peritoneal adhesions provoked by surgery, without administration of vitamin E. Control Group I (CI) included 10 rats which had the abdominal cavity opened without provoking peritoneal adhesions, and vitamin E was administered. Control Group II (CII) included 10 rats which had peritoneal adhesions provoked by surgery, and then lipid based solution was administered intraperitoneally. Control Group III (CIII) included 10 rats which had the abdominal cavity only opened and closed.Groups EI, CI and CII were the subject of the drugs intraperitoneal re-injection in first, second and third day after surgery. The animals were killed during the 8th postoperative day. Macroscopic examination of peritoneal adhesions using the classification reported by Nair was performed and samples for microscopic examination were excised.Results. In group EI peritoneal adhesions were formed in 60% rats (40% weak and 20% solid). In group EII peritoneal adhesions were found in all animals (30% weak and 70% solid). Reduction of the inflammatory response and less severe fibrosis were observed in animals with intraperitoneal administration of vitamin E.Conclusion. In the study, vitamin E administered intraperitoneally to rats decreased the intensity and extensiveness of peritoneal adhesions, which was confirmed by macroscopic and microscopic examinations.
Słowa kluczowe
Wydawca
Rocznik
Tom
82
Numer
10
Strony
547-552
Opis fizyczny
Daty
wydano
2010-10-01
online
2010-12-06
Twórcy
  • Surgery and Emergency Medicine Department, School of Public Health in Bytom, Medical University of Silesia in Katowice
  • General Surgery, Bariatric and Emergency Department, School of Medicine and Division of Dentistry in Zabrze, Medical University of Silesia in Katowice
  • Surgery and Emergency Medicine Department, School of Public Health in Bytom, Medical University of Silesia in Katowice
  • Surgery and Emergency Medicine Department, School of Public Health in Bytom, Medical University of Silesia in Katowice
  • Surgery and Emergency Medicine Department, School of Public Health in Bytom, Medical University of Silesia in Katowice
Bibliografia
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  • Portz DM, Elkins TE, White R et al.: Oxygen free radicals and pelvic adhesion formation: I. Blocking oxygen free radical toxicity to prevent adhesion formation in an endometriosis model. Int J Fertil 1991; 36 (1): 39-42.
  • Roy S, Clark CJ, Mohebali K et al.: Reactive oxygen species and EGR-1 gene expression in surgical postoperative peritoneal adhesions. World J Surg 2004; 28 (3): 316-20.
  • Sanfilippo JS, Booth RJ, Burns CD: Effect of vitamin E on adhesion formation. Reprod Med 1995; 40 (4): 278-82.
  • Tsimoyiannis EC, Tsimoyiannis JC, Sarros CJ et al.: The role of oxygen-derived free radicals in peritoneal adhesion formation induced by ileal ischaemia/reperfusion. Acta Chir Scan 1989; 155: 171-74.
  • Rodgers KE, Girgis W, St Amand K et al.: Reduction of adhesion formation in rabbits by intraperitoneal administration of lazaroid formulations. Hum Reprod 1998; 13 (9): 2443-41.[Crossref]
  • Demetriou AA, Seifter E, Levenson SM: Effect of vitamin A and Citral on peritoneal adhesion formation. J Surg Res 1974; 17 (5): 325-29.[PubMed][Crossref]
  • Galili Y, Ben-Abraham R, Rabau M, Klausner J et al.: Reduction of surgery-induced peritoneal adhesions by methylene blue. Am J Surg 1998; 175 (1): 30-32.[Crossref][PubMed]
  • Hemadeh O, Chilukuri S, Bonet V et al.: Prevention of peritoneal adhesions by administration of sodium carboxymethylcellulose and oral vitamin E. Surgery 1993; 114 (5): 907-10.[PubMed]
  • Kagoma P, Burger SN, Seifter E i Wsp: The effect of vitamin E on experimentally induced peritoneal adhesions in mice. Arch Surg 1985; 120 (8): 949-51.[PubMed][Crossref]
  • Kalfarentzos F, Spiliotis J, Kaklamanis L et al.: Prevention of peritoneal adhesion formation in mice by vitamin E. J R Coll Surg Edinb 1987; 32 (5): 288-90.[PubMed]
  • Konukoglu D, Iynem H, Ziylan E: Antioxidant status in experimental peritonitis: effects of alpha tocopherol and taurolin. Pharmacol Res 1999; 39 (3): 247-51.[Crossref][PubMed]
  • Tuncel P, Gur ES, Kaya E et al.: Effects of vitamin E and cimetidine on peritonitis-induced lipid peroxidation. Res Exp Med 1997; 197 (4): 235-41.
  • de la Portilla F, Ynfante I, Bejarano D et al.: Prevention of peritoneal adhesions by intraperitoneal administration of vitamin E: an experimental study in rats. Dis Colon Rectum 2004; 47 (12): 2157-61.[PubMed]
  • Corrales F, Corrales M, Schirmer CC: Preventing intraperitoneal adhesions with vitamin E and sodium hyauluronate/carboxymethylocellulose: a comparative study in rats. Acta Cir Bras 2008; 23 (10): 36-41.[PubMed]
  • Yetkin G, Uludag M, Citgez B et al.: Prevention of peritoneal adhesions by intraperitoneal administration of vitamin E and human amniotic membrane. Int J Surg 2009, 7 (6):561-65.[PubMed][WoS][Crossref]
  • Uzunkoy A, Akinci OF, Coskun A et al.: Effects of antiadhesive agents on the healing of ointestinal anastomosis. Dis Colon Rectum 2000; 43 (3): 370-75.[Crossref][PubMed]
  • Grzegorczyk K, Rutkowski M, Widerska A: Rola witaminy E jako oksydanta. Prob Ter monitor 2002; 13 (3): 142-47.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_v10035-010-0081-2
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