PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2010 | 82 | 9 | 504-509
Tytuł artykułu

Inhibition of Selected Enzymes with Specific Inhibitors in the Tissue Homogenates from Patients with Colorectal Cancer

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Large intestine malignancy is the second most common malignancy and second leading cause of cancer mortality in Poland. This is related to late detection of these lesions, e.g. due to lack of effective screening tests. Lesions found by a surgeon are clinically advanced, making the treatment often ineffective and sometimes even completely impossible. Discovery of a substance that would be able to stop key processes for the development of malignancy could change such situation. Activity of certain enzymes was found to increase in malignant cells and invasion of malignancy could be triggered by inadequate amount of endogenous inhibitors of these enzymes in the surrounding healthy tissues. Inhibitors identical with that produced in human cells were found in egg whites.The aim of the study was to determine ability of cystatin isolated from egg whites to inhibit activity of cathepsin B and L.Material and methods. Immunohistochemistry and histology of tissue specimen collected from malignant lesions resected from 60 patients diagnosed with large intestine adenocarcinoma, who underwent surgical treatment in 2nd Department of General and Oncological Surgery, Medical University of Wrocław between 2007 and 2009.Results. Differences were fund between health tissues, margins and center of the malignant lesions with regard to amount and distribution of stained cathepsin B - cystatin complexes. The above mentioned inhibitors were able to inhibit 90% of primary activity of cathepsin B and L in malignant tissues.Conclusions. Cystatins obtained from egg whites could be used as substances supporting anti-cancer therapy in the future.
Wydawca
Rocznik
Tom
82
Numer
9
Strony
504-509
Opis fizyczny
Daty
wydano
2010-09-01
online
2010-10-22
Twórcy
autor
  • Department of Surgery, Regional Hospital in Legnica
  • 2 Department of General and Oncological Surgery, Medical University in Wrocław
  • Department of Basic Science Faculty, Public Health Medical University in Wrocław
  • 2 Department of General and Oncological Surgery, Medical University in Wrocław
autor
  • 2 Department of General and Oncological Surgery, Medical University in Wrocław
  • 2 Department of General and Oncological Surgery, Medical University in Wrocław
autor
  • Student Research Group - 2 Department of General and Oncological Surgery, Medical University in Wrocław
  • 2 Department of General and Oncological Surgery, Medical University in Wrocław
Bibliografia
  • Jędrychowski W, Popiela T, Steindorf K: A topographic analysis of athrophic gastritis and stomach cancer risk. A clinicoepidemiological study in Poland. Centr Eur J Publ Hlth 1997; 3; 117-27.
  • Steindorf K, Tobiasz-Adamczyk B, Popiela T: Combined risk assessment of physical activity and dietary habits on the development of colorectal cancer. A hospital-based case-control study in Poland. Eur J Cancer Prev 2000; 9: 309-16.[Crossref][PubMed]
  • Andreyev HJ, Norman R, Cuningham D et al.: Kirsten ras mutations in patients with colorectal cancer: the "RASCAL II" study. Br J of Cancer 2001; 85: 692-96.[Crossref]
  • Costa JC, Cordon-Cardo C: Cancer Diagnosis: Molecular Pathology in Cancer Principles & Practice on Oncology. 6th Ed, DeVita VT, Hellman S, Rosenberg SA. 2002; 617-58.
  • Dillon D, Johnson CC, Topazian MD et al.: The Utility of Ki-ras mutation analysis in the cytologic diagnosis of pancratobiliary neoplasms. Cancer Journal 2000; 6: 294-301.
  • Schmitt M, Janicke F, Graeff H: Tumor-associated proteases. Fibrynolysis 1992; 6: 3-18.
  • Theodoropoulos GE, Panoussopoulos D, Lazaris AC et al.: Evaluation of cathepsin D immunostaining in colorectal adenocarcinoma. J Surg Oncol 1997; 65: 242-48.[Crossref]
  • Curran S, Dundas SR, Buxton J et al.: Matrix metalloproteinase/tissue inhibitors of matrix metalloproteinase phenotype identifies poor prognosis colorectal cancers. Clin Cancer Res 2004; 10; 8229-34.[Crossref]
  • Skrzydlewska E, Sulkowska M, Koda M et al.: Proteolytic-antiproteolytic balance and its regulation in carcinogenesis. World J Gastroenterol 2005; 11: 1251-66.
  • Mikulewicz W, Berdowska I, Jarmułowicz J et al.: Decrease in vivo of cysteine endopeptidases in blood of patients with tumor of larynx. Anti-Cancer Drugs 1993; 4: 341-44.
  • Tamai M, Matsumoto K, Omura S et al.: In vitro and in vivo inhibition of cysteine proteinases by EST, a new analog of E-64. J Pharmacobiodyn 1986; 9: 672-77.[Crossref]
  • Malicka-Błaszkiewicz M, Roth JS: Some factors effecting the ineraction between actin leukaemia L1210 cells and DNase 1. Biochem Biophys Res Commun 1981; 102: 594-601.[Crossref]
  • Kuester D, Lippert H, Roessner A et al.: The cathepsin family and their role in colorectal cancer. Pathol Res Pract 2008; 204: 491-500.[WoS]
  • Kielan W, Saleh Y, Łazarkiewicz B et al.: Cysteine endopeptidases and their autogenous inhibitors in advanced gastric cancer. Bologna (Italy) 3nd International Gastric Cancer Congress Seoul Copyright by Monduzzi Editore SpA 1999; 277-79.
  • Saleh Y, Siewiński M, Kielan W et al.: Regulation of cathepsin B, L expression in vitro in gastric cancer tissues by egg white cystatin. J Exp Ther Oncol 2003; 319-24.
  • Brink M, Weijenberg MP, de Goeij AF et al.: Meat consumption and K-ras mutations in sporadic colon and rectal cancer in The Netherlands Cohort Study. Br J Cancer 2005; 11(92): 1310-20.[Crossref]
  • Schotte P, Van Crekinge W, Van de Craen M et al.: Cathepsin B-mediated activation of proinflammatory caspese-11. Biochem Biophys Res Commun 1998; 251: 379-87.
  • Davis C, Reeves R: High value opportunities from the chicken egg. A report for the Rural Industries Research and Development Corporation. RIRDC Publication No 02/094; RIRDC Project No DAQ - 275A. 2002
  • Siewiński M, Berdowska I, Jarmułowicz J: Sposób otrzymywania inhibitorów cysteinowych peptydaz z roślin i białka jaj. Patent Akademii Medycznej 1998; PL. 174636 B1.
  • Mikulewicz W, Berdowska I, Jarmułowicz J et al.: Decrease in vivo of cysteine endopeptidases in blood of patients with tumor of larynx. Anti-Cancer Drugs 1993; 4: 341-44.
  • Saleh Y, Wnukiewicz J, Trziszka T et al.: Cathepsin B and cysteine protease inhibitors in human tongue cancer: Correlation with tumor staging and in vitro inhibition of cathepsin B by chicken cystatin. J Cancer Molecules 2006; 2: 67-72.
  • Saleh Y, Ziółkowski P, Siewiński M et al.: The combined treatment of transplantable solid mammary carcinona in wistar rats by use photodynamic therapy and cysteine proteinase inhibbitors. In Vivo 2001; 15: 351-57.
  • Sebzda T, Saleh Y, Siewiński M et al.: The influence of vitamin E and human placenta cysteine peptidase inhibitor on the expression of cathepsin B and L implanted hepatoma Morris 5123 tumor model in the Wistar rats. World J Gastroenterol 2005; 11: 587-92.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_v10035-010-0073-2
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.