Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2010 | 82 | 4 | 193-198
Tytuł artykułu

C-Reactive Protein within the Wall of Large Abdominal Aortic Aneurysms; Pathophysiological Implications. A Preliminary Study

Treść / Zawartość
Warianty tytułu
Języki publikacji
The aim of the study was to evaluate the presence and distribution of C-reactive protein (CRP) at the site of the abdominal aortic aneurysm (AAA) suck to determine its potential role in AAA ruptureMaterial and methods. Samples of abdominal aortic aneurysms were obtained from 16 patients during elective surgery. These samples were taken from three places at each three levels of the aneurysmal sack: the neck, at the region of maximum diameter and above bifurcation. The cryostat sections were immunostained for CRP visualization using a specific primary mouse monoclonal anti-C-reactive protein antibody. All the specimens were analyzed morphometrically. The data was analized as the ratio of the active surface of the specimen to its total surface in percentage value.Results. No statistically significant differences between CRP concentrations were found that depended on the site of the aneurysmal sack. However, there exists an average linear correlation between CRP concentration, which is defined as the ratio of the active surface of the specimen to its total surface in percentage value, and the thickness of the adjacent thrombus (Pearson's correlation ratio; r=0.42) with statistical significance of (p<0.00001)Conclusions. The detection of C-reactive protein within the aneurismal wall is not solely sufficient to determine its role in the destruction that eventually leads to a large abdominal aortic aneurysm rupture. It is necessary then to prove an association between not only the metabolism of CRP but also the complement system activation and inflammatory cells response.
Opis fizyczny
  • Department of General, and Vascular Surgery and Angiology, Pomeranian Medical University, Szczecin
  • Department of Histology and Embriology, Pomeranian Medical University, Szczecin
  • Clinic of Paediatric Surgery and Oncology, Pomeranian Medical University, Szczecin
  • Department of General, and Vascular Surgery and Angiology, Pomeranian Medical University, Szczecin
  • Department of General, and Vascular Surgery and Angiology, Pomeranian Medical University, Szczecin
  • Michel J-B, Touat Z, Houard X et al.: New concepts in human abdominal aortic aneurysm pathophysiology: comparison with ascending aortic and other locations. W: Sakalihasan N, Kuivaniemi H, Michel J-P (red) Aortic aneurysms. New insights into an old problem. Les Editions de l'Universite de Liege; 2008, s. 165-85.
  • Platsoucas ChD, Lu S, Nwaneshidu I et al.: Abdominal aortic aneurysm is a specific antigen-driven T cell disease. Ann N Y Acad Sci 2006; 1085: 224-35.
  • Shimizu K, Mitchell RN, Libby P Inflamation and cellular responses in abdominal aortic aneurysms. Arterioscler Thromb Vasc Biol 2006; 26: 987-94.
  • Reynolds GD, Vance RP: C-reactive protein immunohistochemical localization in normal and atherosclerotic human aortas. Arch Pathol Lab Med 1987; 111: 265-69.
  • Sun H, Koika T, Ichikawa TJ et al.: C-reactive protein in atherosclerotic lesions. Its orgin and pathophysiological sigificance. Am J Pathol 2005; 167: 1139-48.
  • Torzewski J, Torzewski M, Bowuer DE et al.: C-reactive protein frequently colocalizes with the terminal complement complex in the intima of early atherosclerotic lesions of human coronary arteries. Arterioscler Thromb Vasc Biol 1998; 18: 1386-92.
  • Verma S, Devaraj S, Jialal I: Is C-rective protein an innocent bystender or proatherogenic culprit. C-rective protein promotes atherothrombosis. Circulation 2006; 113: 2135-50.
  • Buckley DJ, Fu R, Freeman M, Rogers K et al.: C-reactive protein as a risk factor for coronary heart disease: a systemic review and meta-analysis for the U. S. Preventive Services Task Forces. Ann Intern Med 2009; 151: 493-95.
  • Abdellaoui A, Al-Khaffaf H: C-reactive protein (CRP) as a marker in peripheral vascular disease. Eur J Vasc Endovasc Surg 2007; 34: 18-22.[WoS]
  • Hogh AL, Joensen J, Lindholt JS et al.: C-reactive protein predicts future arterial and cardiovascular events in patients with symptomatic peripheral arterial disease. Vascular and Endovascular Surgery 2008; 42: 341-47.[WoS]
  • Norman P, Spencer CA, Lawrence-Brown MM et al.: C-reactive protein levels and the expansion of screen-detected abdominal aortic aneurysms in men. Circulation 2004; 110: 862-66.
  • Domanovits H, Schillinger M, Mullner M et al: Acute phase reactants in patients with abdominal aortic aneurysm. Atherosclerosis 2002; 163: 297-302.
  • Wiernicki I, Cnotliwy M, Baranowska-Bosiacka I et al.: Elastin degradation within abdominal aortic aneurysm wall - relationship between intramural pH and adjacent thrombus formation. Eur J Clin Invest 2008; 38: 883-87.[WoS]
  • Otsu N: A threshold selection method from gray-level histograms. IEEE. Trans Sys Man Cyber 1979; 9: 62-66.
  • Volnakis JE: Human C-reactive protein: expresion, structure, and function. Mol Immunol 2001; 38: 189-97.
  • Yasojima K, Schwab C, McGeer EG et al.: Generation of C-reactive protein and complement components in atherosclerotic plaques. Am J Pathol 2001; 158: 1039-11.
  • Jialal I, Devaraj S, Singh U: Sources of CRP in atherosclerotic lesions. Am J Pathol 2006; 168: 1054-56.
  • Skowasch D, Schrempf S, Preusse CJ et al.: Tissue resident C reactive protein in degenerative aortic valves: correlation with serum C reactive protein concentrations and modification by statins. Heart 2006; 92: 495-98.
  • Baidoshvili A, Nijmeijer R, Lagrand W et al.: Localisation of C reactive protein in infracted tissue sites of multiple organs during sepsis. J Clin Pathol 2002; 55: 152-53.
  • Lagrand WK, Niessen HWM, Wolbink G-J et al.: C-reactive protein colocalizes with complement in human hearts during myocardial infarction. Circulation 1997; 95: 97-103.
  • Vainas T, Lubbers T, Stassen FRM et al.: Serum C-rective protein level is associated with abdominal aortic aneurysm size and may be produced by aneurismal tissue. Circulation 2003; 107: 1103-05.
  • Golledge J, Tsao PS, Dalman RL et al.: Circulating markers of abdominal aortic aneurysm. Presence and progression. Circulation 2008; 118: 2382-92.
  • Allaire E, Schneider F, Saucy F et al.: New insight in aetiopathogenesis of aortic diseases. Eur J Vasc Endovasc Surg 2009; 37: 531-37.[WoS]
  • Treska V, Kocova J, Boudova L: Inflammation in the wall of abdominal aortic aneurysm and its role in the symptomatology of aneurysm. Cytokines Cell Mol Ther 2002; 7: 91-97.
  • Choke E, Cockerill GW, Laing K et al.: Whole genome-expression profiling reveals a role for immune and inflamatory response in abdominal aortic aneurysm rupture. Eur J Vasc Endovasc Surg 2009; 37: 305-10.[WoS]
  • Jones K, Brull D, Brown L: Interleukin-6 and the prognosis of abdominal aortic aneurysms. Circulation 2001; 103: 2260-65.
  • Middleton RK, Lloyd GM, Bown MJ et al.: The pro-inflamatory and chemotactic cytokine micro-enviroment of the abdominal aortic aneurysm wall: a protein array study. J Vasc Surg 2007; 45: 574-80.
  • Darling RC: Ruptured arteriosclerotic abdominal aortic aneurysms. Am J Surg 1970; 119: 397-401.
  • Swedenborg J: The role of the intraluminal thrombus in abdominal aortic aneurysms. W: Sakalihasan N, Kuivaniemi H, Michel J-P (red) Aortic aneurysms. New insights into an old problem. Les Editions de l'Universite de Liege; 2008, s. 343-46.
  • Herron GS, Unemori E, Wang M et al.: Connective tissue proteinases and inhibitors in abdominal aortic aneurysms. Involvment of the vasa vasorum in the pathogenesis of aortic aneurysm. Arterioscler Thromb 1991; 11: 1667-77.
  • Venugopal SK, Devaraj S, Jialal I: Macrophage conditioned medium induces the expression of C-reactive protein in human aortic endothelial cells. Am J Pathol 2005; 166: 1265-71.
  • Kakuta Y, Aoshiba K, Nagai A: C-reactive protein products generated by neutrophil elastase promote neutrophil apoptosis. Arch Med Res 2006; 37: 456-60.
  • Pagano MB, Zhou HF, Ennis TL et al.: Complement-dependent neutrophil recruitment is critical for the development of elastase-induced abdominal aortic aneurysm. Circulation 2009; 119: 1805-13.[WoS]
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.