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2010 | 82 | 1 | 47-52
Tytuł artykułu

Expression of Genes Encoding Aldosterone Synthesis Pathway Enzymatic Proteins in Adrenocortical Adenomas in Patients With Conn Syndrome

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Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
One of the possibilities of explanation of persistent hypertension after adrenocortical adenoma excision in patients with Conn syndrome could be analysis of transcriptional activity of genes encoding aldosterone synthesis pathway enzymatic proteins within these tumours.The aim of the study was to analyze the expression of mentioned genes in adrenocortical adenomas in patients with primary hiperaldosteronism and to analyse correlations between their expression and results of hormonal tests of blood serum before surgery as well as maximal values of blood pressure before surgery and their decrease after operation.Material and methods. Thirteen patients with Conn syndrome subjected to surgical excision of adrenocortical tumour were analyzed. Expression of CYP 11A1, CYP 21A2, CYP 11B2 and HSD 3B2 genes in tissue of removed tumours was analyzed using RT real-time PCR method. GAPDH was used as reference gene. The results analysis was based on amplification threshold values CT. Analysis of clinical consequences of adrenalectomy was performed after a period of minimum 12 months after surgery.Results. Expression of mRNA, presented as 2-ΔΔCT, of CYP 11A1 gene was 1.58 (1.01-2.48), CYP 21A2-2.74 (1.78-4.24), CYP 11B2- 19.15 (11.28-32.50) and HSD 3B2-2.57 (1.47-4.50). Correlation analysis revealed only negative correlation between CYP 11A1 gene expression and plasma renin activity in upright position (r=-0.604; p=0.049) and positive correlation between HSD 3B2 gene expression and decrease of maximal values of systolic blood pressure in long term follow up (r=0.763; p=0.010).Conclusions. High expression of genes encoding aldosterone synthesis pathway enzymatic proteins is observed in adrenocortical adenomas in patients with Conn syndrome. The transcriptional activity of the genes does not correlate with results of preoperative hormonal tests and is not a prognostic factor of persistent hypertension after adrenal tumour excision.
Wydawca
Rocznik
Tom
82
Numer
1
Strony
47-52
Opis fizyczny
Daty
wydano
2010-01-01
online
2010-02-10
Twórcy
autor
  • Department of General, Vascular and Transplant Surgery, Silesian Medical University, Katowice
  • Department of Biochemistry, Silesian Medical University, Katowice
autor
  • Department of Pathophysiology, Silesian Medical University, Katowice
autor
  • Department of General, Vascular and Transplant Surgery, Silesian Medical University, Katowice
autor
  • Department of Morphology, Silesian Medical University, Katowice
  • Department of Nephrology, Endocrinology and Metabolic Diseases, Silesian Medical University, Katowice
  • Department of Molecular Biology, Silesian Medical University, Katowice
autor
  • Department of General, Vascular and Transplant Surgery, Silesian Medical University, Katowice
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_v10035-010-0008-y
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