PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2007 | 79 | 8 | 540-547
Tytuł artykułu

Effects of Saline and Other Peritoneal Lavage Solutions on the Morphology and Function of in Vitro Mesothelial Cells

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The aim of the study was to evaluate the effects of 0.9% NaCl and other solutions suitable for peritoneal lavage on in vitro viability and fibrinolytic activity of human peritoneal mesothelial cells.Material and methods. Mesothelial cells were isolated from the intra-operatively collected greater omentum specimens and then cultured. Subsequently, eight cultures (n=8) were incubated for six hours with M199 culture medium (control group) or culture medium with the addition of 0.9% NaCl, PD fluid Gambrosol-Trio 10 or Hanks' solution. Immediately after exposure to the studied solutions, the amount of LDH released by the cultured cells was determined. Then, cultured cells were incubated for the next 24 hours; after this period their metabolism and fibrinolytic activity were determined by means of IL-6, t-PA and PAI-1 levels. Levels of these substances were compared to the amount of total cellular protein that was determined simultaneously.Results. Hanks' solution showed no significant influence on cellular culture architecture and mesothelium metabolism. PD fluid Gambrosol-Trio 10 damaged mesothelial cell structure and function as manifested by an increased release of LDH and enhanced t-PA synthesis, as well as decreased production of IL-6 and PAI-1. Exposure to 0.9% NaCl solution was found to cause a disturbance in cellular adhesion without significant structural damage (marked changes in cellular morphology without increased LDH release) and significant metabolism impairment as presented by reduced production of t-PA, IL-6 and PAI-1.Conclusions. Saline solution and PD fluids should not be used for peritoneal lavage. Further investigations are required to identify the least harmful solution for mesothelial cells suitable for application in intra-operative peritoneal lavage.
Wydawca

Rocznik
Tom
79
Numer
8
Strony
540-547
Opis fizyczny
Daty
wydano
2007-08-01
online
2007-11-27
Twórcy
  • Department of General and Vascular Surgery, K. Marcinkowski Medical University in Poznań
  • Department of Pathophysiology, K. Marcinkowski Medical University in Poznań
  • Department of General and Vascular Surgery, K. Marcinkowski Medical University in Poznań
  • Department of Pathophysiology, K. Marcinkowski Medical University in Poznań
Bibliografia
  • Hellebrekers BW, Trimbos-Kemper GC, Bakkum EA et al.: Short-term effect of surgical trauma on rat peritoneal fibrinolytic activity and its role in adhesion formation. Thromb Haemost 2000; 84: 876-81.
  • Holmdahl L, Eriksson E, al-Jabreen M et al.: Fibrinolysis in human peritoneum during operation. Surgery 1996; 119: 701-05.
  • Sulaiman H, Dawson L, Laurent GJ et al.: Role of plasminogen activators in peritoneal adhesion formation. Biochem Soc Trans 2002; 30: 126-31.
  • Ivarsson ML, Holmdahl L, Falk P et al.: Characterization and fibrinolytic properties of mesothelial cells isolated from peritoneal lavage. Scand Clin lab Invest 1998; 58: 195-203.
  • Traczyk Z: Hemostaza. W: Fizjologia człowieka z elementami fizjologii stosowanej i klinicznej. Pod redakcją Z. Traczyka i A. Trzebskiego. PZWL, Warszawa 1995; 439-44.
  • Holmdahl L: The role of fibrinolysis in adhesion formation. Eur J Surg 1997; 577: 24-31.
  • Fukatsu K, Saito H, Han I et al.: The greater omentum is the primary site of neutrophil exudation in peritonitis. J Am Coll Surg 1996; 183: 450-56.
  • van Westreenen M, Mul FJ, Pronk A et al.: Influence of peroperative lavage solutions on peritoneal defence mechanisms in vitro. Eur J Surg 1999; 165: 1066-71.
  • Ambrose NS, Donovan IA, Derges S et al.: The efficacy of peritoneal lavage at elective abdominal operations. Br J Surg 1982; 69: 143-44.
  • Yaacobi Y, Goldberg EP, Habal MB: Effect of Ringer's lactate irrigation on the formation of postoperative abdominal adhesions. J Invest Surg 1991; 4: 31-36.
  • van Westreenen M, van den Tol PM, Pronk A et al.: Perioperative lavage promotes intraperitoneal adhesion in the rat. Eur Surg Res 1999; 31: 196-201.
  • Kappas AM, Fatouros M, Papadimitriou K et al.: Effect of intraperitoneal saline irrigation at different temperatures on adhesion formation. Br J Surg 1988; 75: 854-56.
  • Bręborowicz A, Oreopoulos DG: Is normal saline harmful to the peritoneum? Perit Dial Int 2005; 25 (Suppl. 4): 67-70.
  • Abbasoglu O, Sayek I, Hascelik G: The effect of peritoneal lavage on peritoneal cellular defense mechanisms. Acta Chir Belg 1994; 94: 321-24.
  • Shushan A, Mor-Yosef S, Avgar A et al.: Hyaluronic acid for preventing experimental postoperative intraperitoneal adhesions. J Reprod Med 1994; 39: 398-402.
  • Kutlay J, Ozer Y, Isik B et al.: Comparative effectiveness of several agents for preventing postoperative adhesions. World J Surg 2004; 28: 662-65.
  • Maleckas A, Daubaras V, Vaitkus V et al.: Increased postoperative peritoneal adhesion formation after the treatment of experimental peritonitis with chlorhexidine. Langenbecks Arch Surg 2004; 389: 256-60.
  • Jorres A, Gahl GM, Frei U: Peritoneal dialysis fluid biocompatibility: does it really matter? Kidney Int 1994; 48: 79-86.
  • Ito T, Yorioka N, Yamamoto M et al.: Effect of glucose on intercellular junctions of cultured human peritoneal mesothelial cells. J Am Soc Nephrol 2000; 11: 1969-79.
  • Bird SD, Walker RJ: Effects of bicarbonate buffered dialysate on human peritoneal mesothelial cell intracellular calcium homeostasis. Nephrology 2003; 8: 150-55.
  • Gotloib L, Wajsbrot V, Shostak A: Osmotic agents hamper mesothelial repopulation as seen in the doughnut in vivo model. Perit Dial Int 2005; 25: 26-30.
  • Shostak A, Wajsbrot V, Gotloib L: High glucose accelerates the life cycle of the in vivo exposed mesothelium. Kidney Int 2000; 58: 2044-52.
  • W: Schein M, Saada R: Peritonitis: contamination and Infection, Principles of Treatment 95-101. (W) Schein M, Rogers PN (red): Schein's Common Sense Emergency Abdominal Surgery, Springer 2005.
  • Yao V, Platell C, Hall J C: Lavage enhances the production of proinflammatory mediators by peritoneal mesothelial cells in an experimental model. Dis Colon Rectum 2005; 48: 560.
  • Lin Ch: Peritoneal lavage with distilled water during liver resection in patients with spontaneously ruptured hepatocellular carcinomas. J Surg Oncol 2006; 94: 255-56.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_v10035-007-0084-9
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.