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The Electron Paramagnetic Resonance (EPR) was used to investigate free radicals in the peripheral blood of patients subjected to angioplasty treatment. To detect these free radicals, a nitrosobenzene spin trap was used in this experiment. The EPR spectra of the blood with a spin trap conglomerate was measured at room temperature and at 170 K. To confirm the kind of free radicals in the conglomerate blood-spin trap, simulation and quantum-chemical calculations were made, and the conglomerate spin trap with ascorbic acid was measured. Two different types of radicals, one at room temperature and the other in a frozen sample of blood, were found.
Słowa kluczowe
Rocznik
Tom
Numer
Strony
1-12
Opis fizyczny
Daty
wydano
2008-01-01
online
2008-12-29
Twórcy
autor
- Faculty of Physics, Medical Physics Division, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland
autor
- Faculty of Physics, Medical Physics Division, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland
autor
- Regional Hospital, Kielce, Poland
autor
- Faculty of Physics, Medical Physics Division, Adam Mickiewicz University, Umultowska 85, 61-614 Poznan, Poland
Bibliografia
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- Owenius R, Engstrom M, Lindgren M, Huber M. Influence of Solvent Polarity and Hydrogen Bonding on the EPR Parameters of a Nitroxide Spin Label Studied by 9-GHz EPR Spectroscopy and DFT Calculations. J Phys Chem A. 2001; 105: 10967-10977.
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- Zweier JL, Flaherty JT, Weisfeldt ML. Direct measurement of free radical generation following reperfusion of ischemic myocardium. Proc Natl Acad Sci USA. 1987; 84: 1404-1407.[Crossref]
- Zweier JL, Kuppusamy P, Williams R, Rayburn BK, Smith D, Weisfeldt ML, Flaherty JT. Measurement and Characterization of Postischemic Free Radical Generation in the Isolated Perfused Heart. J Biol Chem. 1989; 264(32): 18890-18895.
Typ dokumentu
Bibliografia
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bwmeta1.element.-psjd-doi-10_2478_v10013-008-0001-5