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2011 | 6 | 2 | 181-184
Tytuł artykułu

The effect of a leukodepletion model on the activation stage of platelets

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The preparation of thrombocyte concentrates with filtration before storage (in-line) makes it possible to avoid the presence of mononuclear cells in the concentrate and proinflammatory cytokines. Therefore, this filtration may result with decreased activation of trombocyte receptors in vitro, which may improve therapeutic efficiancy. Methods. We compared two groups, each with 30 therapeutic doses of concentrated thrombocytes. We prepared the first group using the classic model from the buffy coat and the other with concentrated thrombocyte samples filtrated during sampling, so-called in-line, with the WBC filter Imuflex (Terumo). Mononuclear cells (MNC), thrombocyte, and erythrocyte counts in the units of concentrated thrombocytes were obtained on an automatic cell counter, and we used flow cytometry to measure the expression of surface thrombocyte receptors. The results demonstrated that the trombocytes prepared with pre-storage filtration contained a very low level of mononuclear cells and markedly reduced trombocyte receptors. Conclusion. The number of MNC and expression of surface thrombocyte receptors were markedly lower in the concentrated thrombocyte units prepared with in-line filtration. The thrombocytes prepared in this way contain fewer mononuclear cells, are of higher quality, are more functional, and may produce a better therapeutic effect in vivo.
Wydawca
Czasopismo
Rocznik
Tom
6
Numer
2
Strony
181-184
Opis fizyczny
Daty
wydano
2011-04-01
online
2011-02-17
Twórcy
  • Clinic of Hematology, Clinical Centre Nis, University in Nis, 18000, Nis, Serbia , buca.v@nadlanu.com
autor
  • Clinic of Hematology, Clinical Centre Nis, University in Nis, 18000, Nis, Serbia
  • Clinic of Hematology, Clinical Centre Nis, University in Nis, 18000, Nis, Serbia
  • Clinic of Hematology, Clinical Centre Nis, University in Nis, 18000, Nis, Serbia
  • Clinic of Hematology, Clinical Centre Nis, University in Nis, 18000, Nis, Serbia
Bibliografia
  • [1] Bubel S., Wilhelm D., Entelmann N., Kirchner H., Kutler., Chemokines in stored platelet concentrates, Transfusion., 1996; 36, 445–449 http://dx.doi.org/10.1046/j.1537-2995.1996.36596282589.x[Crossref]
  • [2] Bordin JO., Hedlle NM., Bjachman MA, Biological effects of leukocytes presented in transfused cellularr blood products, Blood., 1994, 84, 1703–21.
  • [3] Maly MA, Hadacova I, Hajek P, Zemanek D, Veselka J. High-residual platelet activity despite dual antiplatelet treatment associated with subacute stent trombosis. Cent Eur J Med, 2009; 4, 119–124. http://dx.doi.org/10.2478/s11536-009-0007-8[WoS][Crossref]
  • [4] Salehiomran A, Ahmadi H, Karimi A, Tazik M, Dowlatshahi S, Fathollahi S, Abbasi S. Transfusion associated in-hospital mortality and morbidity in isolated coronary artery bypass surgery. Cent Eur J Med, 2009; 4. 286–292. http://dx.doi.org/10.2478/s11536-009-0044-3[WoS][Crossref]
  • [5] Metcalfe P., Williamson LM., Reutelingsperger CP, Activation during preparation of therapeutic platelets afects deterioration during storage, Comparative Flow cytometric study of different product methods, B J Haematology., 1997, 98, 86–95 http://dx.doi.org/10.1046/j.1365-2141.1997.1572983.x[Crossref]
  • [6] British Commitee for Standards in Haematology, Blood Transfusion Taskforce. Guidlines on the clinical use of leukocyte-depleted blood components, Transfusion-Medicine., 1998,8,59–71 http://dx.doi.org/10.1046/j.1365-3148.1998.00129.x[Crossref]
  • [7] Wadhawa M., Kraildashiri P., Dilger P., Gaines R, Cytokine levels as perfomance indicators for white blood cell reduction of platelet concentrates, Vox Sanguinis., 2002,83,125–136 http://dx.doi.org/10.1046/j.1423-0410.2002.00203.x[Crossref]
  • [8] Schuerholz T., Losche W., Keil O., Friedrich L., Simon T., Marx G, Acute short-term hyperglycemia impairs platelet receptor expression even in healty adults in vitro, Med Sci Monit., 2008, 14,12,BR,294–298
  • [9] Murasaki K., Kawana M., Murasaki S., Tsurumi Y., Tonoue K., Hagiwara N., Kasanuki H, High P-selectin expression and low CD36 occupancy on circulating platelets are strong predictors of restenosis after coronary stenting in patients with coronary artery disease, Heart Vessels., 2007,22,229–236 http://dx.doi.org/10.1007/s00380-006-0966-5[WoS][Crossref]
  • [10] Paunovic D., Van Der Meer P., Kjeldsen-Kragh R., Larson S et al, Multicenter evaluation of whole-blood filter that saves platelets, Transfusion.,2004,44,8,1197–203 http://dx.doi.org/10.1111/j.1537-2995.2004.03350.x[Crossref]
  • [11] Lozano M., Perez-Ceballos E., Rivera J., Paunovic D., et al, Evaluation of a new wholw-blood filter that allows preparation of platelet concentrates by platelet -rich plasma method, Transfusion., 2003,43,1723–1728 http://dx.doi.org/10.1046/j.0041-1132.2003.00587.x[Crossref]
  • [12] Marquardt L., Ruf A., Mansmann U., Winter R., Schuler M., Buggle F., Mayer H., Grau AJ, Course of platelet activation markers after ischemic stroke, Stroke., 2002, 33, 2570–2574 http://dx.doi.org/10.1161/01.STR.0000034398.34938.20[Crossref]
  • [13] Harrison P., Ault KA., Chapman S., Charie L., Davis B., Fuj Marquardt L., Ruf A., Mansmann U., Winter R., Schuler M., Buggle F., Mayer H., Grau AJ, Course of platelet activation markers after ischemic stroke, Stroke., 2002, 33, 2570–2574 http://dx.doi.org/10.1161/01.STR.0000034398.34938.20[Crossref]
  • [14] Krailadsiri P., Seghachian J., Lorna M, Platelet storage lesion of WBC-reduced pooled buffy coat-derived platelet concentrates prepared in three in-process filter-storage bag combinations, Transfusion., 2001,41,243–250 http://dx.doi.org/10.1046/j.1537-2995.2001.41020243.x[Crossref]
  • [15] Balint B., Vucetic D., Trajkovic-Lakic Z., Petakov M et al, Quantitative, functional, morfological and ultrastructural recovery of platelets as predictor for criopreservation, Haematolog(Budap)., 2002,32,4,363–75
  • [16] Metzelaar M.J., Wijngaard PL., Peters PJ., Sixma J.J., Niuwenhius HK., & Clevers HC, CD 63 antigen. A novel lysosomal membrane glycoprotein, cloned by a screening procedure for intracellular antigens in in eucaryotic cells, B Biol Chem.,1991, 266, 3239–3245
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.-psjd-doi-10_2478_s11536-010-0062-1
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